AB201968

Anti-Desmoglein 3/PVA antibody [6G2C11]

Name: Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) | Abcam
Brand: Abcam Limited
SKU: AB201968
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Desmoglein 3/PVA antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DSG3 aa 50-200.

View Alternative Names

CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA

6 Images

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Flow cytometric analysis of A431 cells labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution (green). Negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemical analysis of Paraffin-embedded Human esophageal cancer tissue labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution followed by DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemical analysis of Paraffin-embedded Human esophageal tissue labeling Desmoglein 3/PVA with ab201968 at 1/200 dilution followed by DAB staining.

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Human Desmoglein 3/PVA recombinant (aa55-159)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 108 kDa

false

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

All lanes:

A431 cell lysate

Predicted band size: 108 kDa

false

Supplier Data

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

(Expected MW is 37.5 kDa)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (ab201968) at 1/500 dilution

All lanes:

Human Desmoglein 3/PVA recombinant (aa55-159) protein

Predicted band size: 108 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6G2C11

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human DSG3 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P32926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.

Biological function summary

This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.

Pathways

Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.

Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed : 31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed : 9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed : 22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed : 31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed : 31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed : 22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed : 22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed : 26763450).

See full target information DSG3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1871 PubMed39984440

2025

Activation of CAMK2 by pseudokinase PEAK1 represents a targetable pathway in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Xiuquan Ma,Tianyue Zhao,David R Croucher,Elizabeth V Nguyen,Kimberley C Clark,Changyuan Hu,Sharissa L Latham,Charles Bayly-Jones,Bao V Nguyen,Srikanth Budnar,Sung-Young Shin,Lan K Nguyen,Thomas R Cotton,Anderly C Chüeh,Terry C C Lim Kam Sian,Margaret M Stratton,Andrew M Ellisdon,Roger J Daly

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 3/PVA antibody [6G2C11]

Flow Cytometry - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Western blot - Anti-Desmoglein 3/PVA antibody [6G2C11] (AB201968)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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