Rabbit Recombinant Monoclonal Desmoglein 3/PVA antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [EPR14101] - BSA and Azide free (AB250705), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-epr14101-bsa-and-azide-free-ab250705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87487.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [EPR14101] - BSA and Azide free (AB250705)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed:31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed:9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed:22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed:31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed:31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed:22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed:22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed:26763450).
Alternative names
CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA
Recommended products
Rabbit Recombinant Monoclonal Desmoglein 3/PVA antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14101
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250705 is the carrier-free version of Anti-Desmoglein 3/PVA antibody [EPR14101] ab183743.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.
- Biological function summary
This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.
- Pathways
Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.
- Associated diseases and disorders
Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [EPR14101] - BSA and Azide free (ab250705), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-epr14101-bsa-and-azide-free-ab250705--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87487.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [EPR14101] - BSA and Azide free (ab250705)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [EPR14101] - BSA and Azide free (ab250705)
This data was developed using Anti-Desmoglein 3/PVA antibody [EPR14101] ab183743, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human esophagus, staining Desmoglein 3/PVA with Anti-Desmoglein 3/PVA antibody [EPR14101] ab183743 at 1/250 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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