AB128927

Anti-Desmoglein 3/PVA antibody [EPR7420]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Desmoglein 3/PVA antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA

1 Images

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Western blot - Anti-Desmoglein 3/PVA antibody [EPR7420] (AB128927)

All lanes:

Western blot - Anti-Desmoglein 3/PVA antibody [EPR7420] (ab128927) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 10 µg

Lane 2:

A431 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7420

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.

Biological function summary

This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.

Pathways

Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.

Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed : 31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed : 9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed : 22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed : 31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed : 31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed : 22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed : 22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed : 26763450).

See full target information DSG3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:886226 PubMed35911677

2022

Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis and .

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Rui Wang,Dingfang Bu,Leyi Wang,Yuexin Zhang,Yuan Chang,Chenyang Zhang,Xixue Chen,Xuejun Zhu,Zhi Liu,Mingyue Wang

The Journal of dermatology 44:822-825 PubMed28342216

2017

Case of bullous pemphigoid coexisting with anti-desmoglein autoantibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Duerna Tie,Tokiko Yoshida,Yuko Chinuki,Xia Da,Noriyoshi Ishikawa,Eishin Morita

Scientific reports 6:27114 PubMed27255161

2016

A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Leon Gallegos,Mei Rosa Ng,Mathew E Sowa,Laura M Selfors,Anne White,Ioannis K Zervantonakis,Pragya Singh,Sabin Dhakal,J Wade Harper,Joan S Brugge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 3/PVA antibody [EPR7420]

Western blot - Anti-Desmoglein 3/PVA antibody [EPR7420] (AB128927)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise