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AB118804

Anti-Desmoplakin I+II antibody

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(2 Publications )

Rabbit Polyclonal DESP antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody (AB118804)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody (AB118804)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Demoplakin I+II with ab118804 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Desmoplakin I+II antibody (AB118804)
  • IP

Supplier Data

Immunoprecipitation - Anti-Desmoplakin I+II antibody (AB118804)

Desmoplakin I+II was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab118804 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using a rabbit anti-Desmoplakin antibody which recognizes an upstream epitope at 1 μg/mL.

Lane 1 : ab118804 IP in HeLa whole cell lysate.

Lane 2 : rabbit anti-Desmoplakin antibody recognizing upstream epitope IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-Desmoplakin I+II antibody (ab118804)

Predicted band size: 331 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab118804 was affinity purified using an epitope specific to Desmoplakin I+II immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoplakin is a critical component of the cell structure acting as a link between desmosomes and the intermediate filament network. It exists in two isoforms Desmoplakin I and Desmoplakin II with molecular masses of approximately 332 kDa and 260 kDa respectively. Desmoplakin is mainly expressed in tissues with high mechanical stress such as the heart and skin where strong cell adhesion is necessary. Alternate names like Desmoplakine may also be used in some scientific literature.
Biological function summary

Desmoplakin is essential for maintaining cell adhesion and structural integrity within tissues. It serves as part of the desmosomal complex where it anchors intermediate filaments to the desmosomal plaque ensuring the mechanical stability required by tissues under mechanical stress. This function is particularly critical in epithelial cells and cardiac tissue where desmoplakin interacts closely with other desmosomal components to maintain robust cell-to-cell connections.

Pathways

Desmoplakin plays a significant role in the regulation of adhesive structures through its involvement in the intercellular junctional complex pathways. The protein collaborates with other key components of these pathways such as plakoglobin and plakophilins. Its role in these pathways ensures that cell adhesion and stability are maintained across different tissue types which is vital for normal cellular function and organization.

Mutations or dysfunctional interactions of desmoplakin can lead to conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC) and skin disorders such as epidermolysis bullosa simplex. In ARVC desmoplakin-related defects may disrupt interactions with other desmosomal proteins like plakoglobin impacting heart muscle integrity and function. Similarly in epidermolysis bullosa simplex altered desmoplakin function compromises skin cell adhesion leading to increased susceptibility to injury.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 25733715). Critical for cell-cell adhesion in early stage blastocysts and progression through proamniotic cavity formation (By similarity). Not required for preimplantation morphogenic process in blastocysts (By similarity). Required for keratin filament anchoring at the desmosome junction and subsequent organization of the keratin intermediate filament network within the cytoplasm (By similarity). Required for anchoring of desmosomes to the microtubule architecture, via its interaction with NIN (By similarity). Plays a key role in adhesion and organization of the dermal epithelial barrier (By similarity). Critical for the maintenance of the neural tube structure following formation and organization of the neuroepithelium (By similarity). Facilitates outgrowth and repair of motor neuron fibers in regenerating axons following injury, probably by promoting recruitment of a complex containing DSP, CDH2, VIM and JUP to the outgrowth tips (By similarity). Required for the normal formation of the heart, also required for development of vascular capillary structures and intact endothelial cell barriers (By similarity). Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity). Maintains cardiac rhythmicity by ensuring correct cell-cell adhesion within the sinoatrial node, via stabilization of protein components of both desmosome and Gap junctions (By similarity). Involved in maintaining the protein stability and recruitment of GJA1 to functional gap junctions, via inhibition of KRAS-mediated MAPK1/MAPK3 phosphorylation of GJA1 (By similarity). Required for the survival and maintenance of germ cells in the gonads during embryonic development (By similarity). Binds to telomere DNA (via C-terminus) and acts to prevent telomere damage and maintain telomere length via its interaction with TRF2 (PubMed : 31595153).
See full target information DSP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 15:2350-2362 PubMed31595153

2019

Nuclear localization of Desmoplakin and its involvement in telomere maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Li,Yuan Meng,Yuan Wang,Jingjing Li,Manting Lam,Li Wang,Li-Jun Di

EMBO molecular medicine 6:1593-609 PubMed25319547

2014

5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Madhuri Bhuvanagiri,Joe Lewis,Kerstin Putzker,Jonas P Becker,Stefan Leicht,Jeroen Krijgsveld,Richa Batra,Brad Turnwald,Bogdan Jovanovic,Christian Hauer,Jana Sieber,Matthias W Hentze,Andreas E Kulozik
View all publications
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Product promise

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