JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB16434

Anti-Desmoplakin I+II antibody [DP2.15]

4

(3 Reviews)

|

(33 Publications)

Mouse Monoclonal DESP antibody. Suitable for ICC, WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Chicken, Human, Cow samples. Cited in 33 publications.

View Alternative Names

Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DP2.15

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Chicken, Cow, Human

Applications

ICC/IF, IHC-Fr, ICC, WB

applications

Specificity

The clone number has been updated from (2Q400) to (DP2.15) both clone numbers name the same antibody clone.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

Product details

Suitable for use in the detection of primary and metastatic carcinomas.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoplakin is a critical component of the cell structure acting as a link between desmosomes and the intermediate filament network. It exists in two isoforms Desmoplakin I and Desmoplakin II with molecular masses of approximately 332 kDa and 260 kDa respectively. Desmoplakin is mainly expressed in tissues with high mechanical stress such as the heart and skin where strong cell adhesion is necessary. Alternate names like Desmoplakine may also be used in some scientific literature.
Biological function summary

Desmoplakin is essential for maintaining cell adhesion and structural integrity within tissues. It serves as part of the desmosomal complex where it anchors intermediate filaments to the desmosomal plaque ensuring the mechanical stability required by tissues under mechanical stress. This function is particularly critical in epithelial cells and cardiac tissue where desmoplakin interacts closely with other desmosomal components to maintain robust cell-to-cell connections.

Pathways

Desmoplakin plays a significant role in the regulation of adhesive structures through its involvement in the intercellular junctional complex pathways. The protein collaborates with other key components of these pathways such as plakoglobin and plakophilins. Its role in these pathways ensures that cell adhesion and stability are maintained across different tissue types which is vital for normal cellular function and organization.

Mutations or dysfunctional interactions of desmoplakin can lead to conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC) and skin disorders such as epidermolysis bullosa simplex. In ARVC desmoplakin-related defects may disrupt interactions with other desmosomal proteins like plakoglobin impacting heart muscle integrity and function. Similarly in epidermolysis bullosa simplex altered desmoplakin function compromises skin cell adhesion leading to increased susceptibility to injury.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity).
See full target information DSP

Additional targets

DSP
style
left-column

Publications (33)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37895093

2023

PTPN13 Participates in the Regulation of Epithelial-Mesenchymal Transition and Platinum Sensitivity in High-Grade Serous Ovarian Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leticia Aptecar,Carole Puech,Evelyne Lopez-Crapez,Marion Peter,Peter Coopman,Véronique D'Hondt,Gilles Freiss

eLife 11: PubMed35256050

2022

Placental uptake and metabolism of 25(OH)vitamin D determine its activity within the fetoplacental unit.

Applications

Unspecified application

Species

Unspecified reactive species

Brogan Ashley,Claire Simner,Antigoni Manousopoulou,Carl Jenkinson,Felicity Hey,Jennifer M Frost,Faisal I Rezwan,Cory H White,Emma M Lofthouse,Emily Hyde,Laura D F Cooke,Sheila Barton,Pamela Mahon,Elizabeth M Curtis,Rebecca J Moon,Sarah R Crozier,Hazel M Inskip,Keith M Godfrey,John W Holloway,Cyrus Cooper,Kerry S Jones,Rohan M Lewis,Martin Hewison,Spiros D D Garbis,Miguel R Branco,Nicholas C Harvey,Jane K Cleal

Journal of cell science 134: PubMed34633031

2021

The long non-coding RNA ET-20 mediates EMT by impairing desmosomes in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meera Saxena,Mizue Hisano,Melanie Neutzner,Maren Diepenbruck,Robert Ivanek,Kirti Sharma,Ravi K R Kalathur,Thomas R Bürglin,Salvatore Risoli,Gerhard Christofori

Immunity 54:2072-2088.e7 PubMed34320366

2021

Resident cardiac macrophages mediate adaptive myocardial remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole R Wong,Jay Mohan,Benjamin J Kopecky,Shuchi Guo,Lixia Du,Jamison Leid,Guoshuai Feng,Inessa Lokshina,Oleksandr Dmytrenko,Hannah Luehmann,Geetika Bajpai,Laura Ewald,Lauren Bell,Nikhil Patel,Andrea Bredemeyer,Carla J Weinheimer,Jessica M Nigro,Attila Kovacs,Sachio Morimoto,Peter O Bayguinov,Max R Fisher,W Tom Stump,Michael Greenberg,James A J Fitzpatrick,Slava Epelman,Daniel Kreisel,Rajan Sah,Yongjian Liu,Hongzhen Hu,Kory J Lavine

Environmental health perspectives 129:27005 PubMed33605785

2021

Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Elvis Ticiani,Jeremy Gingrich,Yong Pu,Mathew Vettathu,Jacquelyn Davis,Denny Martin,Margaret G Petroff,Almudena Veiga-Lopez

International journal of molecular sciences 22: PubMed33445576

2021

Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Fraichard,Fidéline Bonnet-Serrano,Christelle Laguillier-Morizot,Marylise Hebert-Schuster,René Lai-Kuen,Jeanne Sibiude,Thierry Fournier,Marie Cohen,Jean Guibourdenche

Theranostics 10:1016-1032 PubMed31938048

2020

PTPN13 induces cell junction stabilization and inhibits mammary tumor invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Hamyeh,Florence Bernex,Romain M Larive,Aurélien Naldi,Serge Urbach,Joelle Simony-Lafontaine,Carole Puech,William Bakhache,Jérome Solassol,Peter J Coopman,Wiljan J A J Hendriks,Gilles Freiss

Molecular and cellular endocrinology 499:110586 PubMed31539598

2019

Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation.

Applications

Unspecified application

Species

Unspecified reactive species

C Fraichard,F Bonnet,A Garnier,M Hébert-Schuster,A Bouzerara,P Gerbaud,I Ferecatu,T Fournier,I Hernandez,S Trabado,J Guibourdenche

FASEB journal : official publication of the Federa 33:12873-12887 PubMed31499012

2019

Galectin-1 interacts with the human endogenous retroviral envelope protein syncytin-2 and potentiates trophoblast fusion in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Toudic,Amandine Vargas,Yong Xiao,Guillaume St-Pierre,Norbert Bannert,Julie Lafond,Éric Rassart,Sachiko Sato,Benoit Barbeau

Nature genetics 51:1389-1398 PubMed31477929

2019

A pooled single-cell genetic screen identifies regulatory checkpoints in the continuum of the epithelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

José L McFaline-Figueroa,Andrew J Hill,Xiaojie Qiu,Dana Jackson,Jay Shendure,Cole Trapnell
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB109445

Anti-Desmoplakin antibody [EPR4383(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (AB109445)

  • 4
  • 2
Primary Antibodies

AB118804

Anti-Desmoplakin I+II antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody (AB118804)

  • 0
  • 0
Primary Antibodies

AB71690

Anti-Desmoplakin I+II antibody

Western blot - Anti-Desmoplakin I+II antibody (AB71690)

  • 4
  • 2

Complementary Products

Primary Antibodies

AB151402

Anti-Plakophilin 2/PKP2 antibody [8H6]

Flow Cytometry - Anti-Plakophilin 2/PKP2 antibody [8H6] (AB151402)

  • 4
  • 2
Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

  • 5
  • 27
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB184919

Anti-gamma Catenin antibody [EPR17310]

RabMAb|Recombinant|KO Validated

Western blot - Anti-gamma Catenin antibody [EPR17310] (AB184919)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com