Rabbit Recombinant Monoclonal DESP antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427849.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427848.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427847.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427846.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427845.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (AB316761)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | WB | IP | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity).
Alternative names
Desmoplakin, DP
Recommended products
Rabbit Recombinant Monoclonal DESP antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28426-66
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316761 is the carrirer-free version of Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoplakin is a critical component of the cell structure acting as a link between desmosomes and the intermediate filament network. It exists in two isoforms Desmoplakin I and Desmoplakin II with molecular masses of approximately 332 kDa and 260 kDa respectively. Desmoplakin is mainly expressed in tissues with high mechanical stress such as the heart and skin where strong cell adhesion is necessary. Alternate names like Desmoplakine may also be used in some scientific literature.
- Biological function summary
Desmoplakin is essential for maintaining cell adhesion and structural integrity within tissues. It serves as part of the desmosomal complex where it anchors intermediate filaments to the desmosomal plaque ensuring the mechanical stability required by tissues under mechanical stress. This function is particularly critical in epithelial cells and cardiac tissue where desmoplakin interacts closely with other desmosomal components to maintain robust cell-to-cell connections.
- Pathways
Desmoplakin plays a significant role in the regulation of adhesive structures through its involvement in the intercellular junctional complex pathways. The protein collaborates with other key components of these pathways such as plakoglobin and plakophilins. Its role in these pathways ensures that cell adhesion and stability are maintained across different tissue types which is vital for normal cellular function and organization.
- Associated diseases and disorders
Mutations or dysfunctional interactions of desmoplakin can lead to conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC) and skin disorders such as epidermolysis bullosa simplex. In ARVC desmoplakin-related defects may disrupt interactions with other desmosomal proteins like plakoglobin impacting heart muscle integrity and function. Similarly in epidermolysis bullosa simplex altered desmoplakin function compromises skin cell adhesion leading to increased susceptibility to injury.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427849.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat spleen. The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427848.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum. The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427847.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse spleen (PMID: 28123876). The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427846.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum. The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427845.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on intercalated disc of rat cardiac muscle. The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427844.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse stomach. The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427843.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)
This data was developed using Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Desmoplakin I+II with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on intercalated disc of mouse cardiac muscle (PMID: 18519735; PMID: 22152112). The section was incubated with Anti-Desmoplakin I+II antibody [EPR28426-66] ab316760 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427844.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761), expandable thumbnail](https://content.abcam.com/products/images/desmoplakin-iii-antibody-epr28426-66-bsa-and-azide-free-ab316761--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427843.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoplakin I+II antibody [EPR28426-66] - BSA and Azide free (ab316761)")
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