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Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse monoclonal antibody that is used to detect Detyrosinated alpha Tubulin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154), expandable thumbnail
  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (AB254154), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (AB254154), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrIHC-P
Human
Tested
Not recommended
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Rat
Tested
Expected
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/250
Notes

Not recommended for human ICC/IF.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Not recommended for human ICC/IF.

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBA3, TUBA1A, Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain

Recommended products

Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse monoclonal antibody that is used to detect Detyrosinated alpha Tubulin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
AA12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Detyrosinated alpha Tubulin?


Anti-Detyrosinated alpha Tubulin [AA12] - Microtubule Marker (ab254154) specifically detects a band for Detyrosinated alpha Tubulin (UniProt: Q71U36) at a molecular weight of 50kDa.

Recommended positive controls


WB: Human, rat and mouse brain tissue lysate. Human and rat hippocampus tissue lysate.IHC-P: Human, mouse and rat cerebrum tissue.IHC-Fr: Mouse and rat cerebrum tissue.ICC/IF: Mouse primary neuron cells.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Detyrosinated alpha Tubulin also known as detyrosinated tubulin or alpha tubulin is a modified form of the alpha subunit of tubulin. This protein undergoes detyrosination which entails removing the terminal tyrosine residue. Detyrosinated alpha Tubulin has an approximate molecular mass of 50 kDa. It localizes mainly in the cytoplasm and plays an important role in the stability and function of microtubules which are key components of the cytoskeleton. These modifications regulate microtubules' dynamic properties impacting cellular processes such as intracellular transport and cell division.

Biological function summary

Detyrosinated alpha Tubulin impacts important cellular functions. It is an integral part of the microtubule network. Microtubules provide structural support to the cell aiding in intracellular transport and cell shape maintenance. The detyrosination of alpha tubulin contributes to the regulation of microtubule stability which is essential for accurate chromosome segregation during mitosis. Furthermore the detyrosination influences interactions with microtubule-associated proteins and motor proteins affecting their binding affinity and activity.

Pathways

Detyrosinated alpha Tubulin is significant in cellular signaling and transport. It is an important part of the microtubule-dependent transport pathways that facilitate the movement of cellular components including vesicles and organelles. The detyrosination of alpha tubulin influences its interaction with motor proteins such as kinesins and dyneins which are responsible for directional transport along microtubules. Additionally alterations in tubulin detyrosination affect the Wnt signaling pathway impacting cellular processes such as proliferation and differentiation.

Associated diseases and disorders

Alterations in the detyrosination of alpha Tubulin have links to neurodegenerative diseases and cancer. In neurodegenerative disorders abnormal detyrosination can disrupt axonal transport contributing to diseases like Alzheimer's. In cancer increased levels of detyrosinated alpha Tubulin can enhance cell invasiveness and metastasis. The dysregulation of tubulin detyrosination affects cellular interactions with proteins such as tau in neurodegenerative disorders and beta-catenin in cancer implicating these proteins in disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154), expandable thumbnail

    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154)

    Detyrosinated alpha Tubulin Western blot staining using mouse Anti-Detyrosinated alpha Tubulin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse heart (PMID:15899979).

    All lanes: Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa

    Exposure time: 7s

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154), expandable thumbnail

    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154)

    Detyrosinated alpha Tubulin Western blot staining using mouse Anti-Detyrosinated alpha Tubulin antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 3 seconds; Lane 3, 4: 7 seconds.

    All lanes: Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Lane 3: Rat hippocampus tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2μg/ml) dilution (Green). Cytoplasmic staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2μg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2μg/ml) dilution (Green). Cytoplasmic staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2μg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154)

    Detyrosinated alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-Detyrosinated alpha Tubulin antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Detyrosinated A Tubulin with ab254154 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3μm followed by image processing with maximum Z projection. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (ab254154)

    Detyrosinated alpha Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Detyrosinated alpha Tubulin antibody

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

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