Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) is a rabbit polyclonal antibody that is used to detect Detyrosinated alpha Tubulin in Western Blot. Suitable for Human samples.
- Over 80 publications
- Trusted since 2007
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- The Journal of cell biology 223:2023A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingchao Wu,Maximilian W D Raas,Paula Sobrevals Alcaraz,Harmjan R Vos,Eelco C Tromer,Berend Snel,Geert J P L KopsPubMed 37934467
- Nature 622:619-6262023Inhibition of fatty acid oxidation enables heart regeneration in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas BraunPubMed 37758950
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 193:581-5952023The microtubule signature in cardiac disease: etiology, disease stage, and age dependency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSıla Algül,Larissa M Dorsch,Oana Sorop,Aryan Vink,Michelle Michels,Cristobal G Dos Remedios,Michiel Dalinghaus,Daphne Merkus,Dirk J Duncker,Diederik W D Kuster,Jolanda van der VeldenPubMed 37644284
- eLife 12:2023Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas R Knudsen,Kaspar W Persson,Carlos Henriquez-Olguin,Zhencheng Li,Nicolas Di Leo,Sofie A Hesselager,Steffen H Raun,Janne R Hingst,Raphaël Trouillon,Martin Wohlwend,Jørgen F P Wojtaszewski,Martin A M Gijs,Thomas Elbenhardt JensenPubMed 37073948
- iScience 26:1061072023Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArlinda Dullovi,Meryem Ozgencil,Vinothini Rajvee,Wai Yiu Tse,Pedro R Cutillas,Sarah A Martin,Zuzana HořejšíPubMed 36852271
- Cancers 15:2023Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarina T Chang,Keyata N Thompson,Stephen J P Pratt,Julia A Ju,Rachel M Lee,Trevor J Mathias,Makenzy L Mull,David A Annis,Eleanor C Ory,Megan B Stemberger,Michele I Vitolo,Stuart S MartinPubMed 36765843
- The Journal of cell biology 222:2022α-Tubulin detyrosination links the suppression of MCAK activity with taxol cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanilo Lopes,Alexandre L Seabra,Bernardo Orr,Helder MaiatoPubMed 36459065
- Basic research in cardiology 117:532022Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyte.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander K Salomon,Sai Aung Phyo,Naima Okami,Julie Heffler,Patrick Robison,Alexey I Bogush,Benjamin L ProsserPubMed 36326891
- Cell death & disease 13:8562022Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfeng Hu,Qiu Xie,Xiang Wu,Weizhen Liu,DongFang Li,Chen Li,WangXing Zhao,LinLin Chen,Zihui Zheng,GuangMing Li,Jun GuoPubMed 36209218
- Communications biology 5:8752022Human alveolar progenitors generate dual lineage bronchioalveolar organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Hoffmann,Benedikt Obermayer,Katja Hönzke,Diana Fatykhova,Zeynep Demir,Anna Löwa,Luiz Gustavo Teixeira Alves,Emanuel Wyler,Elena Lopez-Rodriguez,Maren Mieth,Morris Baumgardt,Judith Hoppe,Theresa C Firsching,Mario Tönnies,Torsten T Bauer,Stephan Eggeling,Hong-Linh Tran,Paul Schneider,Jens Neudecker,Jens C Rückert,Achim D Gruber,Matthias Ochs,Markus Landthaler,Dieter Beule,Norbert Suttorp,Stefan Hippenstiel,Andreas C Hocke,Mirjana KesslerPubMed 36008580
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information. Database link P68366
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain
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Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) is a rabbit polyclonal antibody that is used to detect Detyrosinated alpha Tubulin in Western Blot. Suitable for Human samples.
- Over 80 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information. Database link P68366
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Detyrosinated alpha Tubulin also known as detyrosinated tubulin or alpha tubulin is a modified form of the alpha subunit of tubulin. This protein undergoes detyrosination which entails removing the terminal tyrosine residue. Detyrosinated alpha Tubulin has an approximate molecular mass of 50 kDa. It localizes mainly in the cytoplasm and plays an important role in the stability and function of microtubules which are key components of the cytoskeleton. These modifications regulate microtubules' dynamic properties impacting cellular processes such as intracellular transport and cell division.
- Biological function summary
Detyrosinated alpha Tubulin impacts important cellular functions. It is an integral part of the microtubule network. Microtubules provide structural support to the cell aiding in intracellular transport and cell shape maintenance. The detyrosination of alpha tubulin contributes to the regulation of microtubule stability which is essential for accurate chromosome segregation during mitosis. Furthermore the detyrosination influences interactions with microtubule-associated proteins and motor proteins affecting their binding affinity and activity.
- Pathways
Detyrosinated alpha Tubulin is significant in cellular signaling and transport. It is an important part of the microtubule-dependent transport pathways that facilitate the movement of cellular components including vesicles and organelles. The detyrosination of alpha tubulin influences its interaction with motor proteins such as kinesins and dyneins which are responsible for directional transport along microtubules. Additionally alterations in tubulin detyrosination affect the Wnt signaling pathway impacting cellular processes such as proliferation and differentiation.
- Associated diseases and disorders
Alterations in the detyrosination of alpha Tubulin have links to neurodegenerative diseases and cancer. In neurodegenerative disorders abnormal detyrosination can disrupt axonal transport contributing to diseases like Alzheimer's. In cancer increased levels of detyrosinated alpha Tubulin can enhance cell invasiveness and metastasis. The dysregulation of tubulin detyrosination affects cellular interactions with proteins such as tau in neurodegenerative disorders and beta-catenin in cancer implicating these proteins in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Western blot - Anti-Detyrosinated alpha Tubulin antibody - Loading Control (ab48389)
Detyrosinated alpha Tubulin Western blot staining using rabbit Anti-Detyrosinated alpha Tubulin antibody
Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
All lanes: Western blot - Anti-Detyrosinated alpha Tubulin antibody - Loading Control (ab48389) at 1/500 dilution
Lane 1: Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 2: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Human Brain Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 28 kDa, 44 kDa, 51 kDa
Exposure time: 1min
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