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AB8401

Anti-DFFB/CAD antibody

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(1 Publication)

Rabbit Polyclonal DFFB/CAD antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Cad, Dffb, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, DNA fragmentation factor 40 kDa subunit, CAD, Caspase-activated DNase, DFF-40

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFFB/CAD antibody (AB8401)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFFB/CAD antibody (AB8401)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling DFFB/CAD with Anti-DFFB/CAD antibody (ab8401) at 5μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-DFFB/CAD antibody (AB8401)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DFFB/CAD antibody (AB8401)

Immunofluorescence of DFFB/CAD in Mouse Kidney cells using ab8401 at 10 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFFB/CAD antibody (AB8401)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFFB/CAD antibody (AB8401)

ab8401 at 2μg/ml staining DFFB/CAD in mouse kidney tissue by IHC

Western blot - Anti-DFFB/CAD antibody (AB8401)
  • WB

Unknown

Western blot - Anti-DFFB/CAD antibody (AB8401)

All lanes:

Western blot - Anti-DFFB/CAD antibody (ab8401) at 2 µg/mL

All lanes:

murine kidney tissue lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DFFB also known as DNA fragmentation factor subunit beta or CAD is a protein with a molecular mass of approximately 40 kDa. It plays an important role in the apoptotic process by acting as a nuclease responsible for cleaving DNA into oligonucleosomal fragments. DFFB is found in various tissues expressing mainly in cells undergoing apoptosis. The interaction with its inhibitor DFFA prevents its activation until the apoptotic signal triggers the pathway for its release and activation resulting in DNA fragmentation.
Biological function summary

DFFB is critical in the execution phase of apoptosis which is the programmed cell death essential to maintaining cellular homeostasis and development. It forms a complex with DFFA its chaperone and inhibitor which regulates its nuclease activity. Apoptosis requires controlled DNA degradation activity which DFFB provides ensuring that cells dismantle DNA in a process safe for the organism. The role of DFFB in apoptosis highlights its importance in normal cell turnover and immune system regulation.

Pathways

DFFB is an essential component of the apoptotic signaling pathway. It aligns with the intrinsic mitochondrial pathway which involves cytochrome c release and subsequent apoptosome formation. Proteins such as caspase-3 activate DFFB by cleaving DFFA facilitating its nuclease function. The careful balance of these pathways and proteins ensures orderly cell death preventing unwanted inflammatory responses or pathological conditions.

DFFB has connections to cancer and neurodegenerative disorders. Dysregulation of its apoptotic function can lead to unchecked cell proliferation contributing to tumorigenesis. Furthermore abnormal DFFB activity is linked to conditions such as Alzheimer's disease where excessive or inappropriate apoptosis occurs. The interplay between DFFB and proteins such as caspase-3 often becomes critical in these disorders highlighting the complex role of apoptosis in both development and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
See full target information Dffb
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14186 PubMed38761001

2024

Elimination of damaged mitochondria during UVB-induced senescence is orchestrated by NIX-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Cavinato,Ines Martic,Sophia Wedel,Annabella Pittl,Rafal Koziel,Regina Weinmmüllner,Markus Schosserer,Brigitte Jenewein,Madhusudhan Reddy Bobbili,Elsa Arcalis,Johannes Haybaeck,Gerhard Pierer,Christian Ploner,Martin Hermann,Nikolaus Romani,Matthias Schmuth,Johannes Grillari,Pidder Jansen-Dürr
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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