AB8408

Anti-DFFB/CAD antibody

Name: Anti-DFFB/CAD antibody (ab8408) | Abcam
Brand: Abcam Limited
SKU: AB8408

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(1 Publication)

Rabbit Polyclonal DFFB/CAD antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DFFB aa 1-50.

View Alternative Names

CAD, DFF2, DFF40, DFFB, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DNA fragmentation factor 40 kDa subunit, Caspase-activated DNase, CPAN, DFF-40

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DFFB/CAD antibody (AB8408)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DFFB/CAD antibody (AB8408)

ab8408 at 5μg/ml staining DFFB/CAD in Jurkat cells by ICC/IF.

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Western blot - Anti-DFFB/CAD antibody (AB8408)

All lanes:

Western blot - Anti-DFFB/CAD antibody (ab8408) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate

Lane 2:

K562 whole cell lysate

Predicted band size: 39 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human DFFB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O76075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DFFB also known as DNA fragmentation factor subunit beta or CAD is a protein with a molecular mass of approximately 40 kDa. It plays an important role in the apoptotic process by acting as a nuclease responsible for cleaving DNA into oligonucleosomal fragments. DFFB is found in various tissues expressing mainly in cells undergoing apoptosis. The interaction with its inhibitor DFFA prevents its activation until the apoptotic signal triggers the pathway for its release and activation resulting in DNA fragmentation.

Biological function summary

DFFB is critical in the execution phase of apoptosis which is the programmed cell death essential to maintaining cellular homeostasis and development. It forms a complex with DFFA its chaperone and inhibitor which regulates its nuclease activity. Apoptosis requires controlled DNA degradation activity which DFFB provides ensuring that cells dismantle DNA in a process safe for the organism. The role of DFFB in apoptosis highlights its importance in normal cell turnover and immune system regulation.

Pathways

DFFB is an essential component of the apoptotic signaling pathway. It aligns with the intrinsic mitochondrial pathway which involves cytochrome c release and subsequent apoptosome formation. Proteins such as caspase-3 activate DFFB by cleaving DFFA facilitating its nuclease function. The careful balance of these pathways and proteins ensures orderly cell death preventing unwanted inflammatory responses or pathological conditions.

DFFB has connections to cancer and neurodegenerative disorders. Dysregulation of its apoptotic function can lead to unchecked cell proliferation contributing to tumorigenesis. Furthermore abnormal DFFB activity is linked to conditions such as Alzheimer's disease where excessive or inappropriate apoptosis occurs. The interplay between DFFB and proteins such as caspase-3 often becomes critical in these disorders highlighting the complex role of apoptosis in both development and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

See full target information DFFB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 477:2213-2233 PubMed35460011

2022

Energetic metabolic reprogramming in Jurkat DFF40-deficient cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Merve Kulbay,Bruno Johnson,Guillaume Ricaud,Marie-Noëlle Séguin-Grignon,Jacques Bernier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DFFB/CAD antibody

Immunocytochemistry/ Immunofluorescence - Anti-DFFB/CAD antibody (AB8408)

Western blot - Anti-DFFB/CAD antibody (AB8408)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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