AB230482

Anti-DFNA5/GSDME antibody

Name: Anti-DFNA5/GSDME antibody. Rabbit polyclonal (ab230482) | Abcam
Brand: Abcam Limited
SKU: AB230482

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(8 Publications)

Rabbit Polyclonal DFNA5/GSDME antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human GSDME conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DFNA5, ICERE1, GSDME, Gasdermin-E, Inversely correlated with estrogen receptor expression 1, Non-syndromic hearing impairment protein 5, ICERE-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFNA5/GSDME antibody (AB230482)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFNA5/GSDME antibody (AB230482)

Formalin-fixed, paraffin-embedded human colon tissue stained for DFNA5/GSDME with ab230482 at a 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DFNA5/GSDME antibody (AB230482)

All lanes:

Western blot - Anti-DFNA5/GSDME antibody (ab230482) at 1/500 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 54 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GSDME conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60443

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DFNA5 also known as GSDME (Gasdermin E) is a protein encoded by the GSDME gene. It has a molecular mass of approximately 59 kDa. The protein is expressed in various tissues including the cochlea in the inner ear and some epithelial tissues. Mechanically DFNA5/GSDME plays a role in inducing cell membrane pore formation leading to cell lysis. This activity relates to its involvement in processes like cell death specifically pyroptosis where the cell undergoes a form of programmed necrosis.

Biological function summary

DFNA5/GSDME serves as a pore-forming protein that facilitates pyroptotic cell death usually upon cleavage by caspase-3. This activity is important for innate immune response and the maintenance of cellular homeostasis. DFNA5/GSDME operates independently and does not form part of large protein complexes. The cleaved form inserts into the lipid bilayer of cell membranes contributing to the execution of cell death particularly under stress conditions or cellular insult.

Pathways

DFNA5/GSDME is involved in the pyroptosis and apoptosis pathways. It plays an essential role in the cellular response to inflammation and stress signals by interacting with caspase-3 a critical protease in the apoptosis pathway. The balance between apoptosis and pyroptosis decides cell fate with DFNA5/GSDME activation tipping towards pyroptotic cell death. The interconnection with other gasdermin family proteins such as GSDMD is notable as they share functional and structural similarities in promoting necrosis-like cell death processes.

Mutations in the DFNA5 gene relate to progressive hearing loss (nonsyndromic sensorineural deafness). Dysregulation of GSDME has also been implicated in cancer where altered expression promotes tumorigenesis or tumor suppression depending on the context. In hearing loss GSDME's interaction with pathways influencing apoptosis highlights its pathogenic role while in cancer connections to caspase-3 show a potential dichotomy in cell survival and cell death regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-E. Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 33852854, PubMed : 35594856, PubMed : 36607699). This form constitutes the precursor of the pore-forming protein : upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed : 28459430).. Gasdermin-E, N-terminal. Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis (PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed : 28459430). Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed : 28045099). Exhibits bactericidal activity (PubMed : 27281216). Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed : 21522185, PubMed : 32188940). Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells : cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (PubMed : 31953257, PubMed : 32188940). May play a role in the p53/TP53-regulated cellular response to DNA damage (PubMed : 16897187).. Gasdermin-E, N-terminal. (Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes.

See full target information GSDME

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 54: PubMed39219277

2024

TREM2, a critical activator of pyroptosis, mediates the anti‑tumor effects of piceatannol in uveal melanoma cells via caspase 3/GSDME pathway

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Jiu,Wenjie Li,Yang Liu,Lin Liu,Hong Lu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404693 PubMed39119834

2024

Methuosis Inducer SGI-1027 Cooperates with Everolimus to Promote Apoptosis and Pyroptosis by Triggering Lysosomal Membrane Permeability in Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Luo,Bing Guan,Xiaoqi Deng,Peide Bai,Haichao Huang,Chaohao Miao,Anran Sun,Zhipeng Li,Dianqiang Yang,Xuegang Wang,Zhiqiang Shao,Yulong Wu,Jinchun Xing,Bin Chen,Tao Wang

Cell reports. Medicine 5:101476 PubMed38508138

2024

Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghe Zhang,Weidong Zhao,Yonggang Zhou,Shengdi Xi,Xiuxiu Xu,Xianghui Du,Xiaohu Zheng,Weiping Hu,Rui Sun,Zhigang Tian,Binqing Fu,Haiming Wei

Nature communications 14:6190 PubMed37794006

2023

MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

He Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai Li

Nature communications 14:929 PubMed36807553

2023

GSDME-mediated pyroptosis promotes the progression and associated inflammation of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wei,Beidi Lan,Tao Zheng,Lin Yang,Xiaoxia Zhang,Lele Cheng,Gulinigaer Tuerhongjiang,Zuyi Yuan,Yue Wu

International journal of biological sciences 18:2949-2961 PubMed35541900

2022

Mitochondrial Protein UCP1 Inhibits the Malignant Behaviors of Triple-negative Breast Cancer through Activation of Mitophagy and Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xia,Changbin Chu,Wanqing Li,Hong Chen,Wenhua Xie,Rui Cheng,Kai Hu,Xi Li

Journal of translational medicine 20:128 PubMed35287671

2022

The monomer TEC of blueberry improves NASH by augmenting tRF-47-mediated autophagy/pyroptosis signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Zhu,Yuan Wen,Qiuling Zhang,Fei Nie,Mingliang Cheng,Xueke Zhao

Journal of hematology & oncology 13:149 PubMed33160389

2020

HMGB1 released from GSDME-mediated pyroptotic epithelial cells participates in the tumorigenesis of colitis-associated colorectal cancer through the ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gao Tan,Chongyang Huang,Jiaye Chen,Fachao Zhi

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Product promise

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Anti-DFNA5/GSDME antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DFNA5/GSDME antibody (AB230482)

Western blot - Anti-DFNA5/GSDME antibody (AB230482)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise