Anti-DFNA5/GSDME antibody [EPR19859] ab215191 is a rabbit monoclonal antibody that is used in DFNA5/GSDME western blotting and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR19859 is the most widely used clone for DFNA5/GSDME on the market and is cited in >210 publications
- Specificity confirmed with GSDME knockout cell line validation
Images
![Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--immunoprecipitation-img650.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img64453.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img15783.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)")
![Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--flow-cytometry-intracellular-img63952.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img175176.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)")
Publications
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- Communications biology 6:12712023The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClotilde C N Renaud,Kilian Trillet,Jane Jardine,Laura Merlet,Ophélie Renoult,Mélanie Laurent-Blond,Zoé Catinaud,Claire Pecqueur,Julie Gavard,Nicolas BidèrePubMed 38102401
- Nature communications 14:79232023Pyroptosis inhibiting nanobodies block Gasdermin D pore formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnja Kopp,Gregor Hagelueken,Isabell Jamitzky,Jonas Moecking,Lisa D J Schiffelers,Florian I Schmidt,Matthias GeyerPubMed 38040708
- PloS one 18:e02941422023TLR2 mediates renal apoptosis in neonatal mice subjected experimentally to obstructive nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaja Wyczanska,Jana Rohling,Ursula Keller,Marcus R Benz,Carsten Kirschning,Bärbel Lange-SperandioPubMed 38015955
- Cell death & disease 14:7732023Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Cristaldi,Marco Buscetta,Maura Cimino,Agnese La Mensa,Maria Rita Giuffrè,Luigi Fiore,Claudia Carcione,Fabio Bucchieri,Francesca Rappa,Claudia Coronnello,Nicolina Sciaraffa,Santina Amato,Tommaso Silvano Aronica,Giovanna Lo Iacono,Alessandro Bertani,Elisabetta Pace,Chiara CipollinaPubMed 38007509
- Cell death & disease 14:7552023NINJ1 is activated by cell swelling to regulate plasma membrane permeabilization during regulated necrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYves Dondelinger,Dario Priem,Jon Huyghe,Tom Delanghe,Peter Vandenabeele,Mathieu J M BertrandPubMed 37980412
- Pharmaceutics 15:2023Targeting 7KCh-Induced Cell Death Response Mediated by p38, P2X7 and GSDME in Retinal Pigment Epithelium Cells with Sterculic Acid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Pariente,Rafael Peláez,Rodrigo Ochoa,Álvaro Pérez-Sala,Ángela Villanueva-Martínez,Miriam Bobadilla,Ignacio M LarráyozPubMed 38004569
- The Journal of cell biology 222:2023Inhibition of lysosome-tethered Ragulator-Rag-3D complex restricts the replication of Enterovirus 71 and Coxsackie A16.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinhui Wang,Zhilin Hu,Wei Zhang,Shuwei Wu,Yongjin Hao,Xia Xiao,Jingjing Li,Xiaoliang Yu,Chengkui Yang,Jingfeng Wang,Huiying Zhang,Feng Ma,Weifeng Shi,Jianwei Wang,Xiaobo Lei,Xiaohu Zhang,Sudan HePubMed 37906052
- Communications biology 6:10712023Inflammatory cell death, PANoptosis, screen identifies host factors in coronavirus innate immune response as therapeutic targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR K Subbarao Malireddi,Ratnakar R Bynigeri,Raghvendra Mall,Jon P Connelly,Shondra M Pruett-Miller,Thirumala-Devi KannegantiPubMed 37864059
- Environmental toxicology 39:289-29820232,2',4,4'-Tetrabromodiphenyl ether and cadmium co-exposure activates aryl hydrocarbon receptor pathway to induce ROS and GSDME-dependent pyroptosis in renal tubular epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYating Sheng,Chengpeng Zhang,Dandan Cai,Guangtao Xu,Shipiao Chen,Weijian Li,Jingjian Dong,Bin Shen,Jie Tang,Long XuPubMed 37705237
- Nature communications 14:53552023Integration of AIEgens into covalent organic frameworks for pyroptosis and ferroptosis primed cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhang,An Song,Qi-Chao Yang,Shu-Jin Li,Shuo Wang,Shu-Cheng Wan,Jianwei Sun,Ryan T K Kwok,Jacky W Y Lam,Hexiang Deng,Ben Zhong Tang,Zhi-Jun SunPubMed 37660063
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
| Transfected cell lysate | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples. |
Species Human | Dilution info 1/1000 | Notes Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples. |
Species Transfected cell lysate | Dilution info 1/1000 | Notes Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples. |
Species Rat | Dilution info 1/1000 | Notes Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/60 | Notes - |
Species Human | Dilution info 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Gasdermin-EPrecursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed:27281216, PubMed:28459430, PubMed:33852854, PubMed:35594856, PubMed:36607699). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:28459430).Gasdermin-E, N-terminalPore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed:27281216, PubMed:28459430, PubMed:32188940, PubMed:33852854, PubMed:35594856). After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis (PubMed:28459430, PubMed:32188940, PubMed:33852854, PubMed:35594856). Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed:28459430). Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed:27281216, PubMed:28459430, PubMed:32188940). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed:28045099). Exhibits bactericidal activity (PubMed:27281216). Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed:21522185, PubMed:32188940). Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells: cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (PubMed:31953257, PubMed:32188940). May play a role in the p53/TP53-regulated cellular response to DNA damage (PubMed:16897187).Gasdermin-E, N-terminal(Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes.
Alternative names
DFNA5, ICERE1, GSDME, ICERE1, DFNA5, Gasdermin-E, Inversely correlated with estrogen receptor expression 1, Non-syndromic hearing impairment protein 5, ICERE-1
Recommended products
Anti-DFNA5/GSDME antibody [EPR19859] ab215191 is a rabbit monoclonal antibody that is used in DFNA5/GSDME western blotting and flow cytometry. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR19859 is the most widely used clone for DFNA5/GSDME on the market and is cited in >210 publications
- Specificity confirmed with GSDME knockout cell line validation
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR19859
- Purification technique
Affinity purification Protein A
- Specificity
Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DFNA5 also known as GSDME (Gasdermin E) is a protein encoded by the GSDME gene. It has a molecular mass of approximately 59 kDa. The protein is expressed in various tissues including the cochlea in the inner ear and some epithelial tissues. Mechanically DFNA5/GSDME plays a role in inducing cell membrane pore formation leading to cell lysis. This activity relates to its involvement in processes like cell death specifically pyroptosis where the cell undergoes a form of programmed necrosis.
- Biological function summary
DFNA5/GSDME serves as a pore-forming protein that facilitates pyroptotic cell death usually upon cleavage by caspase-3. This activity is important for innate immune response and the maintenance of cellular homeostasis. DFNA5/GSDME operates independently and does not form part of large protein complexes. The cleaved form inserts into the lipid bilayer of cell membranes contributing to the execution of cell death particularly under stress conditions or cellular insult.
- Pathways
DFNA5/GSDME is involved in the pyroptosis and apoptosis pathways. It plays an essential role in the cellular response to inflammation and stress signals by interacting with caspase-3 a critical protease in the apoptosis pathway. The balance between apoptosis and pyroptosis decides cell fate with DFNA5/GSDME activation tipping towards pyroptotic cell death. The interconnection with other gasdermin family proteins such as GSDMD is notable as they share functional and structural similarities in promoting necrosis-like cell death processes.
- Associated diseases and disorders
Mutations in the DFNA5 gene relate to progressive hearing loss (nonsyndromic sensorineural deafness). Dysregulation of GSDME has also been implicated in cancer where altered expression promotes tumorigenesis or tumor suppression depending on the context. In hearing loss GSDME's interaction with pathways influencing apoptosis highlights its pathogenic role while in cancer connections to caspase-3 show a potential dichotomy in cell survival and cell death regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--immunoprecipitation-img650.jpg "Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
ab215191 at 1/30 immunoprecipitating DFNA5/GSDME in EMT6 (mouse mammary carcinoma cell line) whole cell lysate.
Lane 1 (input): EMT6 whole cell lysate (10μg)
Lane 2 (+): ab215191 + EMT6 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab215191 in EMT6 whole cell lysate
For western blotting, ab215191 at 1/500. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1,000 dilution
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
EMT6 cells were kindly provided by our collaborator Dr. Feng Shao, NIBS
All lanes: Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Predicted band size: 54 kDa
Exposure time: 55s
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img64453.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Exposure times:
Lane 1: 15s
Lane 2: 3 min
The band around 35kDa is GSDME-NT which might be cleaved by activated caspase‐3 (PMID: 33589596, PMID: 31347748,PMID: 35623158).
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 2: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img15783.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 0.7 µg/mL
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse thymus tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 3min
![Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--flow-cytometry-intracellular-img63952.jpg "Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling DFNA5/GSDME with ab215191 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img175176.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Different batches of ab215191 were tested on SH-SY5Y (Human neuroblastoma epithelial cell) lysate at 0.6 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 55 kDa.
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Predicted band size: 54 kDa
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img162383.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/5000 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
Lane 2: DFNA5/GSDME knockout SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 15s
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img39192.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody is specific to the N-terminus of DFNA5/GSDME.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/10000 dilution
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate at 20 µg
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged DFNA5/GSDME (N-terminal) expression vector, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 37 kDa
Exposure time: 1s
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img152561.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution
All lanes: Human fetal brain lysate at 10 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 3min
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img30342.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).
The EMT6 cell lysate was kindly provided by our collaborator Dr. Feng Shao, NIBS.
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution
Lane 1: EMT6 (mouse mammary carcinoma cell line) whole cell lysate at 10 µg
Lane 2: Mouse brain lysate at 10 µg
Lane 3: Rat brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 3min
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img431692.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time: Lanes 1-3: 10 seconds , Lanes 4-6: 40 seconds.
Both ab215191 and Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal ab221843 show negative result in MCF7, which is also consistent with the predicted expression pattern.
Lanes 1 and 4: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution
Lanes 2 and 5: Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal ab221843) at 1/1000 dilution
Lanes 1 - 3: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lanes 4 - 6: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img415910.jpg "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Image from Wang Y et al., Biochem Biophys Res Commu. 2018;495(1):1418-1425. Fig 4(A).; 10.1016/j.bbrc.2017.11.156 with permission from Elsevier.Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
https://www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications
All lanes: Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution
Lane 1: Wild Type SGC-7901 (human papillomavirus-related endocervical adenocarcinoma cell line) cell lysates
Lane 2: DFNA5/GSDME Knockout SGC-7901 cell lysates
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000 dilution
Predicted band size: 54 kDa
![Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--flow-cytometry-intracellular-img414010.jpg "Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized EMT6 (mouse mammary carcinoma cell line) cell line labeling DFNA5/GSDMEwith ab215191 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
EMT6 cells were kindly provided by our collaborator Dr. Feng Shao, NIBS
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img162383.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img39192.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img152561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img30342.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img431692.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--western-blot-img415910.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
![Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191), expandable thumbnail](https://content.abcam.com/products/images/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191--flow-cytometry-intracellular-img414010.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)")
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