Anti-DGAT1 antibody

• WB

Unknown

Western blot - Anti-DGAT1 antibody (AB54037)

All lanes:

Western blot - Anti-DGAT1 antibody (ab54037) at 2 µg/mL

All lanes:

HepG2 lysate

Predicted band size: 55 kDa

Observed band size: 55 kDa

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• WB

CiteAb

Western blot - Anti-DGAT1 antibody (AB54037)

Western Blotting using Anti-DGAT1 antibody, ab54037. Publication image from Corbet, C. et al., 2020, Nat Commun, 31974393. Legend direct from paper.

TGF-β2 promotes FA uptake and TG accumulation into LD.a–c Abundance of neutral lipids (NL), phospholipids (PL) and free fatty acids (FFA) (a), abundance of saturated and monounsaturated fatty acids (SFA and MUFA, respectively) in the neutral lipid fraction (b), and LD content (c) in native SiHa cells after treatment with 4 ng/ml TGF-β2 for 6 h. d, e14C-palmitate uptake for 10 min in SiHa cells after treatment with 4 ng/ml TGF-β2 for 6 h (d) and in acidosis-adapted SiHa cells following treatment with 10 µM Trabedersen for 7 days in absence or presence of 4 ng/ml TGF-β2 for 24 h (e). f–h Representative immunoblotting for cell surface-localized CD36 and total biotinylated proteins in native and acidosis-adapted SiHa cells following treatment with 4 ng/ml TGF-β2 for 6 h and 24 h (f), following treatment with 10 µM Trabedersen for 7 days or 2 µM SB431542 for 24 h (g) or with 4 ng/ml TGF-β2 and 10 µM PKC-ζ pseudo-substrate inhibitor for 24 h (h). i, j Quantification of surface-localized CD36 in native and acidosis-adapted SiHa cells treated as indicated in (h). k, l mRNA (k) and protein expression of DGAT1 (l) in native and acidosis-adapted tumor cells. m–o Representative immunoblotting for DGAT1 in native and acidosis-adapted SiHa cells following treatment with 10 µM Trabedersen for 7 days or 2 µM SB431542 for 24 h (m), with 10 µM PKC-ζ pseudo-substrate inhibitor for 24 h (n) or with 10 µM GW6471 for 48 h (o). p mRNA expression of PLIN2 in native and acidosis-adapted SiHa cells following treatment with 10 µM TGFβ2-specific antisense oligonucleotide Trabedersen for 7 days. q Co-expression analysis of TGFB2, PLIN1, PLIN2, and PLIN3 genes in human healthy volunteers and colorectal cancer patient samples. Data are represented as mean ± SEM of three independent experiments (with ≥6 technical replicates). Significance was determined by Student’s t-test (c, d, j), by one-way ANOVA (e–i) or two-way ANOVA (a, k, p) with Bonferroni multiple-comparison analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. Source data are provided as a Source Data file.

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