AB59034

Anti-DGAT1 antibody

Name: Anti-DGAT1 antibody (ab59034) | Abcam
Brand: Abcam Limited
SKU: AB59034
Price: 430 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Goat Polyclonal DGAT1 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DGAT1 aa 300-350.

View Alternative Names

AGRP1, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase, ARAT, Retinol O-fatty-acyltransferase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody (AB59034)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody (AB59034)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells labelling DGAT1 with ab59034 for 1 hour at 10 µg/mL. Cells were permeabilized with 0.15% Triton. Anti-Goat IgG H&L (Alexa Fluor^® 488) at 2 µg/mL was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).

Negative control : Unimmunized goat IgG 10 µg/mL followed by Alexa Fluor^® 488 secondary antibody (2ug/ml).

Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm is seen.

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Western blot - Anti-DGAT1 antibody (AB59034)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-DGAT1 antibody (ab59034) at 0.5 µg/mL

All lanes:

Human Duodenum lysate (35µg protein in RIPA buffer)

Predicted band size: 55 kDa

Observed band size: 50 kDa

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Supplier Data

Western blot - Anti-DGAT1 antibody (AB59034)

Primary incubation : 1 hour at room temperature.

All lanes:

Western blot - Anti-DGAT1 antibody (ab59034) at 0.03 µg/mL

All lanes:

Mouse liver lysate in RIPA buffer at 35 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

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CiteAb

Western blot - Anti-DGAT1 antibody (AB59034)

DGAT1 western blot using anti-DGAT1 antibody ab59034. Publication image and figure legend from Kaylor, J. J., Radu, R. A., et al., 2015, PLoS One, PubMed 25974161.

ab59034 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59034 please see the product overview.

DGAT1 expression in retina and RPE.(A) Immunofluorescent analysis of DGAT1 (green) and CRALBP (red) in distal ocular sections from six-month-old BALB/c mice. Nuclei were counter-stained with DAPI (blue). The merged image shows overlapping expression of DGAT1 and CRALBP (yellow). Labels identifying retinal layers are shown to the right of the image. RPE, retinal pigment epithelium; IPM, interphotoreceptor matrix; OLM, outer limiting membrane; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Müller-cell endfeet contact the vitreous within the GCL. Note expression of both DGAT1 and CRALBP in the apical microvilli of Müller cells, extending beyond the OLM into the IPM. (B) Light microscopy of twenty day old primary cultured bovine Müller-cells (scale bar = 100μm, 10X). (C) Expression of MFAT, DGAT1, CRALBP, GFAP, and DES1 mRNA's by qRT-PCR on cDNA from primary-cultured bovine Müller-cell RNA. Levels were normalized to the actin mRNA. CRALBP, GFAP, MFAT and DES1 are positive controls for Müller-cell expression. Error bars show standard deviation of the mean for four (n = 4) independent experiments (p = <0.001, 1-way ANOVA). (D) Immunoblot of dgat1-/- and wild-type (129S2/Sv) mouse eyecup, as well as, HEK-293T cell homogenates transfected with mouse DGAT1 or non-recombinant pcDNA using antisera against mouse DGAT1.

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DGAT1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

O75907

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGAT1 also known as acyl CoA:diacylglycerol acyltransferase 1 is an enzyme with a molecular mass of approximately 55 kDa. DGAT1 is expressed in various tissues with high levels noted in adipose tissue and the intestines. The enzyme catalyzes the final step in triglyceride synthesis by transferring acyl-CoA to diacylglycerol to form triglycerides. This process is essential in lipid metabolism impacting both energy storage and membrane synthesis.

Biological function summary

DGAT1 plays a significant role in lipid homeostasis and energy balance across multiple biological systems. This enzyme is key in the conversion of dietary fats into stored energy and it does not exist as part of a complex. DGAT1's activity contributes to the maintenance of cellular lipid droplets and it has implications therefore in conditions where lipid metabolism is disrupted.

Pathways

DGAT1 is a part of the triglyceride biosynthesis pathway an important route within the lipid metabolism framework. In this context the enzyme works alongside other lipogenic proteins such as acetyl-CoA carboxylase and fatty acid synthase to synthesize triglycerides. DGAT1's role in this pathway has implications for how the body manages fat storage and utilization linking it to the regulation of systemic energy levels.

Aberrant DGAT1 activity links to metabolic conditions such as obesity and type 2 diabetes. Alterations in DGAT1 expression can affect lipid storage and mobilization contributing to these disorders. Within this pathogenic context DGAT1 also interacts with proteins like adipose triglyceride lipase which further influences the breakdown of stored triglycerides impacting adipose tissue accumulation and insulin sensitivity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed : 16214399, PubMed : 18768481, PubMed : 28420705, PubMed : 32433610, PubMed : 32433611, PubMed : 9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed : 18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed : 16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed : 18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed : 16214399). Exhibits additional acyltransferase activities, includin acyl CoA : monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).

See full target information DGAT1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed33525494

2021

Metabolic Transition of Milk Triacylglycerol Synthesis in Response to Varying Levels of Three 18-Carbon Fatty Acids in Porcine Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yantao Lv,Fang Chen,Shihai Zhang,Jun Chen,Yinzhi Zhang,Min Tian,Wutai Guan

Frontiers in immunology 10:1860 PubMed31456800

2019

A Novel Role for Triglyceride Metabolism in Foxp3 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Duncan Howie,Annemieke Ten Bokum,Stephen Paul Cobbold,Zhanru Yu,Benedikt M Kessler,Herman Waldmann

Nutrition & metabolism 13:48 PubMed27478484

2016

Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Zeina Soayfane,François Tercé,Michela Cantiello,Horst Robenek,Michel Nauze,Valérie Bézirard,Sophie Allart,Bruno Payré,Florence Capilla,Christel Cartier,Christine Peres,Talal Al Saati,Vassilia Théodorou,David W Nelson,Chi-Liang Eric Yen,Xavier Collet,Christine Coméra

PloS one 10:e0125921 PubMed25974161

2015

Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium.

Applications

Species

Unspecified reactive species

Joanna J Kaylor,Roxana A Radu,Nicholas Bischoff,Jacob Makshanoff,Jane Hu,Marcia Lloyd,Shannan Eddington,Tran Bianconi,Dean Bok,Gabriel H Travis

Arteriosclerosis, thrombosis, and vascular biology 29:767-73 PubMed19286631

2009

TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Insa Buers,Horst Robenek,Stefan Lorkowski,Yvonne Nitschke,Nicholas J Severs,Oliver Hofnagel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DGAT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody (AB59034)

Western blot - Anti-DGAT1 antibody (AB59034)

Western blot - Anti-DGAT1 antibody (AB59034)

Western blot - Anti-DGAT1 antibody (AB59034)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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