Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

Intracellular Flow Cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1 : 100 dilution (4.3 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1/400 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

• IP

Supplier Data

Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

All lanes:

Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr13430-4-n-terminal-ab181180'>ab181180</a>)

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (AB240250)

ab181180 (purified) at 1 : 30 dilution (2μg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
Lane 1 (input) : Human fetal kidney lysate 10μg
Lane 2 (+) : ab181180 & Human fetal kidney lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

All lanes:

Immunoprecipitation - Anti-DGAT1 antibody [EPR13430-4] - N-terminal (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr13430-4-n-terminal-ab181180'>ab181180</a>)

Predicted band size: 55 kDa

false