AB178711

Anti-DGAT1 antibody [EPR13430]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal DGAT1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AGRP1, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase, ARAT, Retinol O-fatty-acyltransferase

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (AB178711)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (AB178711)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DGAT1 with ab178711 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab178711 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] (AB178711)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] (AB178711)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cellslabeling DGAT1 with ab178711 at 1/250 dilution (red) compared withwith a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (AB178711)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling DGAT1 with ab178711 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/1000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa,55 kDa

Observed band size: 18 kDa,55 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (ab178711) at 1/5000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Exposure time: 1min

Carrier free

Anti-DGAT1 antibody [EPR13430] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13430

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGAT1 also known as acyl CoA:diacylglycerol acyltransferase 1 is an enzyme with a molecular mass of approximately 55 kDa. DGAT1 is expressed in various tissues with high levels noted in adipose tissue and the intestines. The enzyme catalyzes the final step in triglyceride synthesis by transferring acyl-CoA to diacylglycerol to form triglycerides. This process is essential in lipid metabolism impacting both energy storage and membrane synthesis.

Biological function summary

DGAT1 plays a significant role in lipid homeostasis and energy balance across multiple biological systems. This enzyme is key in the conversion of dietary fats into stored energy and it does not exist as part of a complex. DGAT1's activity contributes to the maintenance of cellular lipid droplets and it has implications therefore in conditions where lipid metabolism is disrupted.

Pathways

DGAT1 is a part of the triglyceride biosynthesis pathway an important route within the lipid metabolism framework. In this context the enzyme works alongside other lipogenic proteins such as acetyl-CoA carboxylase and fatty acid synthase to synthesize triglycerides. DGAT1's role in this pathway has implications for how the body manages fat storage and utilization linking it to the regulation of systemic energy levels.

Aberrant DGAT1 activity links to metabolic conditions such as obesity and type 2 diabetes. Alterations in DGAT1 expression can affect lipid storage and mobilization contributing to these disorders. Within this pathogenic context DGAT1 also interacts with proteins like adipose triglyceride lipase which further influences the breakdown of stored triglycerides impacting adipose tissue accumulation and insulin sensitivity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed : 16214399, PubMed : 18768481, PubMed : 28420705, PubMed : 32433610, PubMed : 32433611, PubMed : 9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed : 18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed : 16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed : 18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed : 16214399). Exhibits additional acyltransferase activities, includin acyl CoA : monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).

See full target information DGAT1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26035 PubMed40676134

2025

Shift in the urinary metabolome associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin activation of the hepatic aryl hydrocarbon receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Warren J Sink,Russell Fling,Ali Yilmaz,Rance Nault,Delanie Goniwiecha,Jack R Harkema,Stewart F Graham,Tim Zacharewski

Communications biology 6:300 PubMed36944894

2023

SB2301-mediated perturbation of membrane composition in lipid droplets induces lipophagy and lipid droplets ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjoo Jung,Jongbeom Park,Mingi Kim,Jaeyoung Ha,Hana Cho,Seung Bum Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DGAT1 antibody [EPR13430]

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (AB178711)

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] (AB178711)

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] (AB178711)

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Western blot - Anti-DGAT1 antibody [EPR13430] (AB178711)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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