AB250080

Anti-DGAT1 antibody [EPR13430] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DGAT1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

AGRP1, DGAT, DGAT1, Diacylglycerol O-acyltransferase 1, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase, ARAT, Retinol O-fatty-acyltransferase

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DGAT1 with ab178711 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab178711 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DGAT1 with ab178711 at 1/250 dilution (red) compared with with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling DGAT1 with ab178711 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr13430-ab178711'>ab178711</a>) at 1/1000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr13430-ab178711'>ab178711</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa,55 kDa

Observed band size: 18 kDa,55 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

This data was developed using ab178711, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR13430] (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr13430-ab178711'>ab178711</a>) at 1/5000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Exposure time: 1min

Unconjugated

Anti-DGAT1 antibody [EPR13430]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13430

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250080 is the carrier-free version of ab178711.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGAT1 also known as acyl CoA:diacylglycerol acyltransferase 1 is an enzyme with a molecular mass of approximately 55 kDa. DGAT1 is expressed in various tissues with high levels noted in adipose tissue and the intestines. The enzyme catalyzes the final step in triglyceride synthesis by transferring acyl-CoA to diacylglycerol to form triglycerides. This process is essential in lipid metabolism impacting both energy storage and membrane synthesis.

Biological function summary

DGAT1 plays a significant role in lipid homeostasis and energy balance across multiple biological systems. This enzyme is key in the conversion of dietary fats into stored energy and it does not exist as part of a complex. DGAT1's activity contributes to the maintenance of cellular lipid droplets and it has implications therefore in conditions where lipid metabolism is disrupted.

Pathways

DGAT1 is a part of the triglyceride biosynthesis pathway an important route within the lipid metabolism framework. In this context the enzyme works alongside other lipogenic proteins such as acetyl-CoA carboxylase and fatty acid synthase to synthesize triglycerides. DGAT1's role in this pathway has implications for how the body manages fat storage and utilization linking it to the regulation of systemic energy levels.

Aberrant DGAT1 activity links to metabolic conditions such as obesity and type 2 diabetes. Alterations in DGAT1 expression can affect lipid storage and mobilization contributing to these disorders. Within this pathogenic context DGAT1 also interacts with proteins like adipose triglyceride lipase which further influences the breakdown of stored triglycerides impacting adipose tissue accumulation and insulin sensitivity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed : 16214399, PubMed : 18768481, PubMed : 28420705, PubMed : 32433610, PubMed : 32433611, PubMed : 9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed : 18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed : 16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed : 18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed : 16214399). Exhibits additional acyltransferase activities, includin acyl CoA : monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).

See full target information DGAT1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DGAT1 antibody [EPR13430] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Flow Cytometry (Intracellular) - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Western blot - Anti-DGAT1 antibody [EPR13430] - BSA and Azide free (AB250080)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise