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Rabbit Recombinant Monoclonal DGAT1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse samples.

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Images

Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306), expandable thumbnail
  • Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:15834126, PubMed:19028692, PubMed:20876538, PubMed:22493088, PubMed:28420705). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (By similarity). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:15834126). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (By similarity). In contrast to DGAT2 it is not essential for survival (PubMed:11959864). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:19028692). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (PubMed:15834126). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).

Alternative names

Diacylglycerol O-acyltransferase 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase, ARAT, Retinol O-fatty-acyltransferase

Recommended products

Rabbit Recombinant Monoclonal DGAT1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse samples.

Alternative names

Diacylglycerol O-acyltransferase 1, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase, ARAT, Retinol O-fatty-acyltransferase

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28418-75

Purification technique

Affinity purification Protein A

Specificity

A band at ~25 KDa has been observed in Mouse brown fat tissue stored at -80? prior to Western Blotting. The identify of this band is unknown.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317306 is the carrirer-free version of Anti-DGAT1 antibody [EPR28418-75] ab317305.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DGAT1 also known as acyl CoA:diacylglycerol acyltransferase 1 is an enzyme with a molecular mass of approximately 55 kDa. DGAT1 is expressed in various tissues with high levels noted in adipose tissue and the intestines. The enzyme catalyzes the final step in triglyceride synthesis by transferring acyl-CoA to diacylglycerol to form triglycerides. This process is essential in lipid metabolism impacting both energy storage and membrane synthesis.

Biological function summary

DGAT1 plays a significant role in lipid homeostasis and energy balance across multiple biological systems. This enzyme is key in the conversion of dietary fats into stored energy and it does not exist as part of a complex. DGAT1's activity contributes to the maintenance of cellular lipid droplets and it has implications therefore in conditions where lipid metabolism is disrupted.

Pathways

DGAT1 is a part of the triglyceride biosynthesis pathway an important route within the lipid metabolism framework. In this context the enzyme works alongside other lipogenic proteins such as acetyl-CoA carboxylase and fatty acid synthase to synthesize triglycerides. DGAT1's role in this pathway has implications for how the body manages fat storage and utilization linking it to the regulation of systemic energy levels.

Associated diseases and disorders

Aberrant DGAT1 activity links to metabolic conditions such as obesity and type 2 diabetes. Alterations in DGAT1 expression can affect lipid storage and mobilization contributing to these disorders. Within this pathogenic context DGAT1 also interacts with proteins like adipose triglyceride lipase which further influences the breakdown of stored triglycerides impacting adipose tissue accumulation and insulin sensitivity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306), expandable thumbnail

    Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306)

    This data was developed using Anti-DGAT1 antibody [EPR28418-75] ab317305, the same antibody clone in a different buffer formulation.

    The identity of the lower and higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-DGAT1 antibody [EPR28418-75] (Anti-DGAT1 antibody [EPR28418-75] ab317305) at 1/1000 dilution

    Lane 1: Undifferentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: 3T3-L1 differentiated for 6 days whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 103s

    The identity of the lower and higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306), expandable thumbnail

    Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306)

    This data was developed using Anti-DGAT1 antibody [EPR28418-75] ab317305, the same antibody clone in a different buffer formulation.

    Low expression: skeletal muscle, heart (PMID: 10802663).

    The identity of the lower and higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-DGAT1 antibody [EPR28418-75] (Anti-DGAT1 antibody [EPR28418-75] ab317305) at 1/1000 dilution

    Lane 1: Mouse brown fat tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 125s

    Low expression: skeletal muscle, heart (PMID: 10802663).

    The identity of the lower and higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (ab317306)

    This data was developed using Anti-DGAT1 antibody [EPR28418-75] ab317305, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling DGAT1 with Anti-DGAT1 antibody [EPR28418-75] ab317305 at 1/100 (5.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing endoplasmic reticulum staining in differentiated 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-KDEL mouse monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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