Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306)

This data was developed using ab317305, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling DGAT1 with ab317305 at 1/100 (5.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing endoplasmic reticulum staining in differentiated 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Anti-KDEL mouse monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• WB

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306)

This data was developed using ab317305, the same antibody clone in a different buffer formulation.

The identity of the lower and higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR28418-75] (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr28418-75-ab317305'>ab317305</a>) at 1/1000 dilution

Lane 1:

Undifferentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

3T3-L1 differentiated for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-DGAT1 antibody [EPR28418-75] - BSA and Azide free (AB317306)

This data was developed using ab317305, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle, heart (PMID : 10802663).

The identity of the lower and higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DGAT1 antibody [EPR28418-75] (<a href='/en-us/products/primary-antibodies/dgat1-antibody-epr28418-75-ab317305'>ab317305</a>) at 1/1000 dilution

Lane 1:

Mouse brown fat tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 125s