AB237613

Anti-DGAT2 antibody

Name: Anti-DGAT2 antibody. Rabbit polyclonal (ab237613) | Abcam
Brand: Abcam Limited
SKU: AB237613
Price: 410 USD
Availability: InStock

Be the first to review this product! Submit a review

(12 Publications)

Rabbit Polyclonal DGAT2 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DGAT2 aa 100 to C-terminus.

View Alternative Names

HMFN1045, UNQ738/PRO1433, DGAT2, Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, ARAT, Retinol O-fatty-acyltransferase

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (AB237613)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (AB237613)

Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling DHHC-12 with ab237613 at a 1 : 50 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab237613 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Image inset shows absence of staining in secondary antibody only control – uses 1% BSA instead of primary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Human DGAT2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96PD7

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The diacylglycerol O-acyltransferase 2 commonly referred to as DGAT2 plays a mechanical role in triglyceride biosynthesis by catalyzing the final step of triacylglycerol formation from diacylglycerol and acyl-CoA. This protein also functions under the name acyl-CoA:diacylglycerol acyltransferase 2. DGAT2 has a molecular weight of approximately 40-45 kDa. The enzyme is mainly expressed in tissues like the liver adipose tissue and intestines where lipid metabolism is an important function.

Biological function summary

DGAT2 significantly contributes to energy storage and lipid homeostasis in cells. The protein is not usually known to be part of a larger macromolecular complex but it works in association with lipid droplets where the fats are stored. The enzyme's activity significantly impacts the regulation of intracellular triglyceride levels making it important for maintaining energy balance within the cell and the entire organism.

Pathways

The role of DGAT2 is mainly tied to lipid metabolism particularly the triglyceride synthesis pathway. It directly assists in converting excess carbohydrates and other substrates into triglycerides for energy storage. DGAT2 works closely with proteins like DGAT1 another isoform involved in triacylglycerol synthesis and both contribute to maintaining adequate lipid storage and energy regulation within the body.

DGAT2 has implications in metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). An overexpression or increased activity of DGAT2 can lead to excessive lipid formation and storage contributing to these conditions. It shows interaction with proteins like perilipin which are involved in lipid droplet formation influencing pathological fat accumulation in tissues. Low levels or activity of DGAT2 have also been investigated concerning insulin resistance further linking it to metabolic syndrome-related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed : 27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Also functions as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).

See full target information DGAT2

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:405 PubMed38858390

2024

Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Morcillo,Khushbu Kabra,Kevin Velasco,Hector Cordero,Sarah Jennings,Taekyung D Yun,Delfina Larrea,H Orhan Akman,Eric A Schon

Animals : an open access journal from MDPI 13: PubMed37048451

2023

Overexpression of Regulates the Differentiation of Bovine Preadipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pan-Pan Guo,Xin Jin,Jun-Fang Zhang,Qiang Li,Chang-Guo Yan,Xiang-Zi Li

Frontiers in veterinary science 9:847363 PubMed35754541

2022

Expression of Gene and Its Associations With Intramuscular Fat Content and Breast Muscle Fiber Characteristics in Domestic Pigeons ().

Applications

Unspecified application

Species

Unspecified reactive species

Haiguang Mao,Zhaozheng Yin,Mengting Wang,Wenwen Zhang,Sayed Haidar Abbas Raza,Fayez Althobaiti,Lili Qi,Jinbo Wang

International journal of molecular sciences 23: PubMed35409324

2022

Meta-Inflammation and De Novo Lipogenesis Markers Are Involved in Metabolic Associated Fatty Liver Disease Progression in BTBR ob/ob Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Opazo-Ríos,Manuel Soto-Catalán,Iolanda Lázaro,Aleix Sala-Vila,Luna Jiménez-Castilla,Macarena Orejudo,Juan Antonio Moreno,Jesús Egido,Sebastián Mas-Fontao

Nutrients 14: PubMed35334934

2022

Hydrolyzed Bound Phenolics from Rice Bran Alleviate Hyperlipidemia and Improve Gut Microbiota Dysbiosis in High-Fat-Diet Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghe Zhao,Ruifen Zhang,Fei Huang,Lihong Dong,Lei Liu,Xuchao Jia,Jianwei Chi,Yongxuan Ma,Mei Deng,Yanxia Chen,Qin Ma,Mingwei Zhang

Biochemistry. Biokhimiia 86:1407-1417 PubMed34906049

2021

AMPK/PGC-1α/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lin,Yin Jin,Xiang Hu,Erjiong Huang,Qihan Zhu

International journal of molecular sciences 22: PubMed34638947

2021

Leptin Reduces Plin5 mA Methylation through FTO to Regulate Lipolysis in Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Dongqin Wei,Qian Sun,Yizhou Li,Chaowei Li,Xinjian Li,Chao Sun

BMC cancer 21:732 PubMed34174847

2021

Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuojun Liu,Jia Liu,Tianming Zhang,Lin Li,Shuo Zhang,Hao Jia,Yuanshi Xia,Mingxia Shi,Jing Zhang,Shuhua Yue,Xiaofang Chen,Jian Yu

Journal of food and drug analysis 29:375-388 PubMed35696203

2021

Pepsin-digested chicken-liver hydrolysate attenuates hepatosteatosis by relieving hepatic and peripheral insulin resistance in long-term high-fat dietary habit.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Hsieng Samuel Wu,Yi-Ling Lin,Wen-Yuan Yang,Sheng-Yao Wang,Yi-Chen Chen

Journal of neurochemistry 158:482-499 PubMed33905537

2021

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Omar Ramírez-Nuñez,Mariona Jové,Pascual Torres,Joaquim Sol,Laia Fontdevila,Ricardo Romero-Guevara,Pol Andrés-Benito,Victòria Ayala,Chiara Rossi,Jordi Boada,Mònica Povedano,Isidro Ferrer,Reinald Pamplona,Manuel Portero-Otin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DGAT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (AB237613)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise