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AB59493

Anti-DGAT2 antibody

3

(3 Reviews)

|

(10 Publications)

Goat Polyclonal DGAT2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DGAT2 aa 300 to C-terminus.

View Alternative Names

HMFN1045, UNQ738/PRO1433, DGAT2, Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, ARAT, Retinol O-fatty-acyltransferase

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (AB59493)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (AB59493)

ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human DGAT2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96PD7

Specificity

This antibody is not expected to cross-react with other DGAT members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The diacylglycerol O-acyltransferase 2 commonly referred to as DGAT2 plays a mechanical role in triglyceride biosynthesis by catalyzing the final step of triacylglycerol formation from diacylglycerol and acyl-CoA. This protein also functions under the name acyl-CoA:diacylglycerol acyltransferase 2. DGAT2 has a molecular weight of approximately 40-45 kDa. The enzyme is mainly expressed in tissues like the liver adipose tissue and intestines where lipid metabolism is an important function.
Biological function summary

DGAT2 significantly contributes to energy storage and lipid homeostasis in cells. The protein is not usually known to be part of a larger macromolecular complex but it works in association with lipid droplets where the fats are stored. The enzyme's activity significantly impacts the regulation of intracellular triglyceride levels making it important for maintaining energy balance within the cell and the entire organism.

Pathways

The role of DGAT2 is mainly tied to lipid metabolism particularly the triglyceride synthesis pathway. It directly assists in converting excess carbohydrates and other substrates into triglycerides for energy storage. DGAT2 works closely with proteins like DGAT1 another isoform involved in triacylglycerol synthesis and both contribute to maintaining adequate lipid storage and energy regulation within the body.

DGAT2 has implications in metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). An overexpression or increased activity of DGAT2 can lead to excessive lipid formation and storage contributing to these conditions. It shows interaction with proteins like perilipin which are involved in lipid droplet formation influencing pathological fat accumulation in tissues. Low levels or activity of DGAT2 have also been investigated concerning insulin resistance further linking it to metabolic syndrome-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed : 27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).
See full target information DGAT2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 13: PubMed38539902

2024

Effect of Macroalga on Non-Alcoholic Fatty Liver Disease in Obese Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Maitane González-Arceo,Leixuri Aguirre,María Teresa Macarulla,Clàudia Gil-Pitarch,María Luz Martínez-Chantar,María P Portillo,Saioa Gómez-Zorita

Animals : an open access journal from MDPI 12: PubMed35883393

2022

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Fang Zhang,Seong-Ho Choi,Qiang Li,Ying Wang,Bin Sun,Lin Tang,En-Ze Wang,Huan Hua,Xiang-Zi Li

Disease models & mechanisms 13: PubMed31822493

2020

Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Phillipe D O'Brien,Kai Guo,Stephanie A Eid,Amy E Rumora,Lucy M Hinder,John M Hayes,Faye E Mendelson,Junguk Hur,Eva L Feldman

American journal of physiology. Regulatory, integr 317:R552-R562 PubMed31411897

2019

Impact of leptin deficiency compared with neuronal-specific leptin receptor deletion on cardiometabolic regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jussara M do Carmo,Alexandre A da Silva,Fabio N Gava,Sydney P Moak,Xuemei Dai,John E Hall

Frontiers in immunology 10:1860 PubMed31456800

2019

A Novel Role for Triglyceride Metabolism in Foxp3 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Duncan Howie,Annemieke Ten Bokum,Stephen Paul Cobbold,Zhanru Yu,Benedikt M Kessler,Herman Waldmann

American journal of physiology. Endocrinology and 316:E578-E589 PubMed30694691

2019

Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shilpa R Nagarajan,Moumita Paul-Heng,James R Krycer,Daniel J Fazakerley,Alexandra F Sharland,Andrew J Hoy

Molecular cancer research : MCR 17:949-962 PubMed30647103

2019

Extracellular Fatty Acids Are the Major Contributor to Lipid Synthesis in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Seher Balaban,Zeyad D Nassar,Alison Y Zhang,Elham Hosseini-Beheshti,Margaret M Centenera,Mark Schreuder,Hui-Ming Lin,Atqiya Aishah,Bianca Varney,Frank Liu-Fu,Lisa S Lee,Shilpa R Nagarajan,Robert F Shearer,Rae-Anne Hardie,Nikki L Raftopulos,Meghna S Kakani,Darren N Saunders,Jeff Holst,Lisa G Horvath,Lisa M Butler,Andrew J Hoy

Journal of dairy science 102:833-845 PubMed30415861

2018

Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Hongdou Jia,Xiaobing Li,Guowen Liu,Juan J Loor,Ryan Bucktrout,Xudong Sun,Guojin Li,Xin Shu,Jihong Dong,Yazhe Wang,Rankun Zuo,Zhe Wang,Xinwei Li

American journal of physiology. Endocrinology and 315:E949-E960 PubMed29763374

2018

Transgenic overexpression of CTRP3 prevents alcohol-induced hepatic triglyceride accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Trogen,Joshua Bacon,Ying Li,Gary L Wright,Ashley Degroat,Kendra L Hagood,Zachary Warren,Allan Forsman,Aruna Kilaru,W Andrew Clark,Jonathan M Peterson

Gastroenterology 152:1449-1461.e7 PubMed28132890

2017

Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Alonso,David Fernández-Ramos,Marta Varela-Rey,Ibon Martínez-Arranz,Nicolás Navasa,Sebastiaan M Van Liempd,José L Lavín Trueba,Rebeca Mayo,Concetta P Ilisso,Virginia G de Juan,Marta Iruarrizaga-Lejarreta,Laura delaCruz-Villar,Itziar Mincholé,Aaron Robinson,Javier Crespo,Antonio Martín-Duce,Manuel Romero-Gómez,Holger Sann,Julian Platon,Jennifer Van Eyk,Patricia Aspichueta,Mazen Noureddin,Juan M Falcón-Pérez,Juan Anguita,Ana M Aransay,María Luz Martínez-Chantar,Shelly C Lu,José M Mato
View all publications
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