Goat Polyclonal DGAT2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human DGAT2 aa 300 to C-terminus.
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- Animals : an open access journal from MDPI 12:2022Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Fang Zhang,Seong-Ho Choi,Qiang Li,Ying Wang,Bin Sun,Lin Tang,En-Ze Wang,Huan Hua,Xiang-Zi LiPubMed 35883393
- Disease models & mechanisms 13:2020Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhillipe D O'Brien et. al.PubMed 31822493
- American journal of physiology. Regulatory, integr 317:R552-R5622019Impact of leptin deficiency compared with neuronal-specific leptin receptor deletion on cardiometabolic regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJussara M do Carmo et. al.PubMed 31411897
- Frontiers in immunology 10:18602019A Novel Role for Triglyceride Metabolism in Foxp3 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuncan Howie et. al.PubMed 31456800
- American journal of physiology. Endocrinology and 316:E578-E5892019Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShilpa R Nagarajan et. al.PubMed 30694691
- Molecular cancer research : MCR 17:949-9622019Extracellular Fatty Acids Are the Major Contributor to Lipid Synthesis in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeher Balaban et. al.PubMed 30647103
- Journal of dairy science 102:833-8452018Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongdou Jia,Xiaobing Li,Guowen Liu,Juan J Loor,Ryan Bucktrout,Xudong Sun,Guojin Li,Xin Shu,Jihong Dong,Yazhe Wang,Rankun Zuo,Zhe Wang,Xinwei LiPubMed 30415861
- American journal of physiology. Endocrinology and 315:E949-E9602018Transgenic overexpression of CTRP3 prevents alcohol-induced hepatic triglyceride accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Trogen et. al.PubMed 29763374
- Gastroenterology 152:1449-1461.e72017Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Alonso et. al.PubMed 28132890
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DGAT2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PD7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg/mL | Notes Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed:27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
Alternative names
HMFN1045, UNQ738/PRO1433, DGAT2, Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, ARAT, Retinol O-fatty-acyltransferase
Recommended products
Goat Polyclonal DGAT2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human DGAT2 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DGAT2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PD7
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is not expected to cross-react with other DGAT members.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The diacylglycerol O-acyltransferase 2 commonly referred to as DGAT2 plays a mechanical role in triglyceride biosynthesis by catalyzing the final step of triacylglycerol formation from diacylglycerol and acyl-CoA. This protein also functions under the name acyl-CoA:diacylglycerol acyltransferase 2. DGAT2 has a molecular weight of approximately 40-45 kDa. The enzyme is mainly expressed in tissues like the liver adipose tissue and intestines where lipid metabolism is an important function.
- Biological function summary
DGAT2 significantly contributes to energy storage and lipid homeostasis in cells. The protein is not usually known to be part of a larger macromolecular complex but it works in association with lipid droplets where the fats are stored. The enzyme's activity significantly impacts the regulation of intracellular triglyceride levels making it important for maintaining energy balance within the cell and the entire organism.
- Pathways
The role of DGAT2 is mainly tied to lipid metabolism particularly the triglyceride synthesis pathway. It directly assists in converting excess carbohydrates and other substrates into triglycerides for energy storage. DGAT2 works closely with proteins like DGAT1 another isoform involved in triacylglycerol synthesis and both contribute to maintaining adequate lipid storage and energy regulation within the body.
- Associated diseases and disorders
DGAT2 has implications in metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). An overexpression or increased activity of DGAT2 can lead to excessive lipid formation and storage contributing to these conditions. It shows interaction with proteins like perilipin which are involved in lipid droplet formation influencing pathological fat accumulation in tissues. Low levels or activity of DGAT2 have also been investigated concerning insulin resistance further linking it to metabolic syndrome-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)
ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
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