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AB325121

Anti-DGAT2 antibody [EPR27385-45]

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Rabbit Recombinant Monoclonal DGAT2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

HMFN1045, UNQ738/PRO1433, DGAT2, Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, ARAT, Retinol O-fatty-acyltransferase

4 Images
Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)
  • WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 180 seconds; Lanes 4-5 : 70 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting DGAT2 whole cell lysate at 20 µg

Lane 3:

PAZ6 (human adipose epithelial cell) whole cell lysate at 20 µg

Lane 4:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

3T3-L1 differentiated for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)
  • WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 48 seconds; Lane 4 : 180 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human fat tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 37 kDa

false

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)
  • WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 92 seconds; Lanes 2-5 : 26 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brown fat tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse lung tissue lysate at 20 µg

Lane 6:

Mouse white fat tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)
  • WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 125 seconds; Lanes 2-5 : 59 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Rat skin tissue lysate at 20 µg

Lane 2:

Rat white fat tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27385-45

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed : 27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Also functions as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed : 28420705).
See full target information DGAT2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com