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AB262847

Anti-dGK antibody

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Rabbit Polyclonal dGK antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DGUOK aa 50-150.

View Alternative Names

DGK, DGUOK

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)

Paraffin-embedded Human testis tissue stained for dGK using ab262847 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)

Paraffin-embedded Human smooth muscle tissue stained for dGK using ab262847 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-dGK antibody (AB262847)

Paraffin-embedded Human lymph node tissue stained for dGK using ab262847 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-dGK antibody (AB262847)
  • WB

Supplier Data

Western blot - Anti-dGK antibody (AB262847)

All lanes:

Western blot - Anti-dGK antibody (ab262847) at 0.04 µg/mL

All lanes:

human cell line SCLC-21H

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DGUOK aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q16854

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'dGK' also known as deoxyguanosine kinase is an enzyme that plays an important role in the salvage pathway of nucleotide synthesis. It is a mitochondrial kinase with a known mass of approximately 29 kDa. The enzyme catalyzes the phosphorylation of purine deoxyribonucleosides such as deoxyguanosine to their monophosphate forms. dGK is expressed mainly in mitochondria-rich tissues like the liver muscle and brain indicating its importance in nucleoside metabolism in these areas.
Biological function summary

Deoxyguanosine kinase contributes significantly to the maintenance of mitochondrial DNA by ensuring the supply of deoxyribonucleotide triphosphates (dNTPs). It does not function as part of a larger protein complex working independently to convert purine deoxynucleosides in a rate-limiting step. The enzyme ensures that cellular processes requiring DNA replication and repair can proceed highlighting its importance in cellular functionality and homeostasis.

Pathways

Deoxyguanosine kinase participates in the nucleotide salvage pathway which is pivotal for recycling nucleosides into nucleotide pools. This pathway complements the de novo synthesis pathway providing a rapid response mechanism for nucleotide demand. dGK operates alongside other kinases like thymidine kinase which similarly act to phosphorylate nucleosides. This interconnection facilitates the balance of nucleotide pools within the cell important for effective DNA synthesis and repair.

Mutations or deficiencies in deoxyguanosine kinase can lead to mitochondrial DNA depletion syndromes. Such conditions result in a variety of clinical manifestations including muscle weakness and neurological issues. dGK is linked to disorders like mitochondrial neurogastrointestinal encephalopathy where faulty dNTP pools cause progressive multisystemic symptoms. The disturbance in deoxyguanosine kinase’s function emphasizes its role in maintaining cellular energy homeostasis and genome integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine (PubMed : 11687801, PubMed : 17073823, PubMed : 23043144, PubMed : 8692979, PubMed : 8706825). In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2. Phosphorylates certain nucleoside analogs (By similarity). Widely used as target of antiviral and chemotherapeutic agents.
See full target information DGUOK
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Product promise

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