AB88672

Anti-DGKA antibody

Name: Anti-DGKA antibody (ab88672) | Abcam
Brand: Abcam Limited
SKU: AB88672
Price: 545 USD
Availability: InStock

(1 Review)

(1 Publication)

Mouse Polyclonal DGKA antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DGKA.

View Alternative Names

DAGK, DAGK1, DGKA, Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-DGKA antibody (AB88672)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DGKA antibody (AB88672)

Immunofluorescence staining of DGKA in HeLa cells using ab88672 at 10 μg/ml.

Unknown

Western blot - Anti-DGKA antibody (AB88672)

All lanes:

Western blot - Anti-DGKA antibody (ab88672) at 1 µg/mL

Lane 1:

DGKA transfected 293T cell lysate at 25 µg

Lane 2:

non transfected lysate at 25 µg

Predicted band size: 83 kDa

Observed band size: 81 kDa

false

Unknown

Western blot - Anti-DGKA antibody (AB88672)

All lanes:

Western blot - Anti-DGKA antibody (ab88672) at 1 µg/mL

All lanes:

Human spleen lysate at 50 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DGKA.

P23743

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKA also known as diacylglycerol kinase alpha plays an important role in the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by transferring a phosphate group from ATP. This enzymatic process regulates levels of DAG and PA which are critical molecules for signaling processes. DGKA has a molecular weight of approximately 80 kDa. Expression of DGKA occurs in various tissues with notable presence in the brain and immune system highlighting its significance in neurological and immunological functions.

Biological function summary

DGKA modulates multiple signaling pathways by controlling the availability of DAG and PA essential intermediates in intracellular signaling. It operates as part of a complex that interacts with various phosphorylated proteins to affect downstream signaling. The enzyme influences cellular processes such as proliferation differentiation and apoptosis by modulating DAG-dependent signaling pathways.

Pathways

DGKA integrates into significant signaling cascades including the PI3K/Akt and MAPK pathways. In the PI3K/Akt pathway DGKA modulates the activity of proteins like PKC through its DAG-regulating function. Alternatively in the MAPK pathway it influences protein phosphorylation through effects on upstream kinases. This regulation highlights the enzyme's role in critical cell fate decisions.

DGKA has associations with certain cancers and immune-related diseases. Aberrant DGKA activity links to oncogenic processes influencing tumor growth and progression via its impact on the PI3K/Akt pathway. In immune disorders dysregulated DGKA activity connects to excessive inflammation affecting proteins such as PKC that are involved in immune responses. Understanding DGKA's role in these diseases could drive new therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed : 15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed : 22627129).

See full target information DGKA

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 188:2545-55 PubMed22331067

2012

IκB kinase β is required for activation of NF-κB and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells.

Applications

Unspecified application

Species

Human

Elisa Lupino,Cristina Ramondetti,Marco Piccinini

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DGKA antibody

Immunocytochemistry/ Immunofluorescence - Anti-DGKA antibody (AB88672)

Western blot - Anti-DGKA antibody (AB88672)

Western blot - Anti-DGKA antibody (AB88672)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise