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AB218039

Anti-DGKE antibody

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(2 Publications)

Rabbit Polyclonal DGKE antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DGKE aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DAGK5, DGKE, Diacylglycerol kinase epsilon, DAG kinase epsilon, Diglyceride kinase epsilon, DGK-epsilon

1 Images
Western blot - Anti-DGKE antibody (AB218039)
  • WB

Supplier Data

Western blot - Anti-DGKE antibody (AB218039)

All lanes:

Western blot - Anti-DGKE antibody (ab218039) at 1/300 dilution

All lanes:

mouse testis lysates

Predicted band size: 64 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DGKE aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P52429

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKE also known as diacylglycerol kinase epsilon is an enzyme that catalyzes the phosphorylation of diacylglycerol (DAG) to produce phosphatidic acid. DGKE has a molecular mass of approximately 63 kDa. It shows expression in multiple tissues with notable presence in kidney placenta and lung. In particular DGKE is found in endothelial cells and certain immune cells. DGKE functions as a significant regulator of lipid signaling influencing the balance of diacylglycerol and phosphatidic acid within cells.
Biological function summary

DGKE plays an important role in cellular signaling processes that involve DAG and phosphatidic acid. It is not known to be a component of a larger complex but acts independently to mediate its functions. By regulating DAG levels DGKE impacts several cellular processes such as membrane trafficking cytoskeletal reorganization and activation of protein kinase C. The enzyme also contributes to controlling cell growth and apoptosis by modulating downstream signaling pathways.

Pathways

DGKE is significantly involved in the phosphoinositide signaling pathway and the glycerophospholipid metabolism pathway. In these pathways DGKE interacts with other DAG kinases and phospholipase C. Through these interactions DGKE influences the generation of inositol trisphosphate and diacylglycerol which are important second messengers. Its role supports cellular responses to external stimuli thereby affecting cell proliferation differentiation and survival.

DGKE mutations have been linked to nephrotic syndrome particularly a type associated with early-onset disease called DGKE-associated nephrotic syndrome. Moreover DGKE is connected to thrombosis because its deficiency can lead to abnormal blood clotting. CD2AP another protein involved in the actin cytoskeleton and important for kidney function interacts with DGKE and is often studied together in the context of glomerular diseases. DGKE's relationship with these conditions highlights its importance in vascular and kidney health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 19744926, PubMed : 21477596, PubMed : 22108654, PubMed : 23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 8626589). Also plays an important role in the biosynthesis of complex lipids (PubMed : 8626589). Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed : 19744926, PubMed : 22108654, PubMed : 23274426). Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed : 22108654).
See full target information DGKE
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 81:101887 PubMed38280449

2024

Renal tubule-specific Atgl deletion links kidney lipid metabolism to glucagon-like peptide 1 and insulin secretion independent of renal inflammation or lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Maria F Fernandes,Juan J Aristizabal-Henao,Phillip M Marvyn,Iman M'Hiri,Meghan A Wiens,Monica Hoang,Manuel Sebastian,Renato Nachbar,Philippe St-Pierre,Kalsha Diaguarachchige De Silva,Geoffrey A Wood,Jamie W Joseph,Christine A Doucette,André Marette,Ken D Stark,Robin E Duncan

International journal of clinical and experimental 11:99-109 PubMed31938091

2018

polysaccharide extracts alleviate MPP-induced PC-12 cell injury through inhibition of Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiting Liu,Xiaowan Li
View all publications
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Product promise

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