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Rabbit Recombinant Monoclonal DGKE antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:19744926, PubMed:22108654, PubMed:21477596, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:8626589, PubMed:15544348). Also plays an important role in the biosynthesis of complex lipids (PubMed:8626589). Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed:19744926, PubMed:22108654, PubMed:23274426). Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed:22108654).

Alternative names

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Rabbit Recombinant Monoclonal DGKE antibody. Carrier free. Suitable for WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR7847

Purification technique

Affinity purification Protein A

Dissociation constant

1.09 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248242 is the carrier-free version of ab128949.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

DGKE plays an important role in cellular signaling processes that involve DAG and phosphatidic acid. It is not known to be a component of a larger complex but acts independently to mediate its functions. By regulating DAG levels DGKE impacts several cellular processes such as membrane trafficking cytoskeletal reorganization and activation of protein kinase C. The enzyme also contributes to controlling cell growth and apoptosis by modulating downstream signaling pathways.

Activity summary

DGKE also known as diacylglycerol kinase epsilon is an enzyme that catalyzes the phosphorylation of diacylglycerol (DAG) to produce phosphatidic acid. DGKE has a molecular mass of approximately 63 kDa. It shows expression in multiple tissues with notable presence in kidney placenta and lung. In particular DGKE is found in endothelial cells and certain immune cells. DGKE functions as a significant regulator of lipid signaling influencing the balance of diacylglycerol and phosphatidic acid within cells.

Pathways

DGKE is significantly involved in the phosphoinositide signaling pathway and the glycerophospholipid metabolism pathway. In these pathways DGKE interacts with other DAG kinases and phospholipase C. Through these interactions DGKE influences the generation of inositol trisphosphate and diacylglycerol which are important second messengers. Its role supports cellular responses to external stimuli thereby affecting cell proliferation differentiation and survival.

Associated diseases and disorders

DGKE mutations have been linked to nephrotic syndrome particularly a type associated with early-onset disease called DGKE-associated nephrotic syndrome. Moreover DGKE is connected to thrombosis because its deficiency can lead to abnormal blood clotting. CD2AP another protein involved in the actin cytoskeleton and important for kidney function interacts with DGKE and is often studied together in the context of glomerular diseases. DGKE's relationship with these conditions highlights its importance in vascular and kidney health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242), expandable thumbnail

    Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242)

    This data was developed using ab128949, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    We recommend to use 1% SDS Hot lysis prepare method to get desired western blot results.

    All lanes: Western blot - Anti-DGKE antibody [EPR7847] (AB128949) at 1/1000 dilution

    All lanes: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 64 kDa

    Observed band size: 64 kDa

    This data was developed using ab128949, the same antibody clone in a different buffer formulation.
    ** Blocking and dilution buffer:** 5% NFDM/TBST.
    We recommend to use 1% SDS Hot lysis prepare method to get desired western blot results.

  • Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242), expandable thumbnail

    Western blot - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242)

    This data was developed using ab128949, the same antibody clone in a different buffer formulation.

    The cell lysates were prepared in 1%SDS Hot lysis method.

    All lanes: Western blot - Anti-DGKE antibody [EPR7847] (AB128949) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 64 kDa

    Observed band size: 64 kDa

  • OI-RD Scanning - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242), expandable thumbnail

    OI-RD Scanning - Anti-DGKE antibody [EPR7847] - BSA and Azide free (ab248242)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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