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AB151967

Anti-DGKG antibody

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(3 Publications)

Rabbit Polyclonal DGKG antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DGKG aa 450-700.

View Alternative Names

DAGK3, DGKG, Diacylglycerol kinase gamma, DAG kinase gamma, Diglyceride kinase gamma, DGK-gamma

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKG antibody (AB151967)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKG antibody (AB151967)

Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling DGKG with ab151967 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DGKG antibody (AB151967)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DGKG antibody (AB151967)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling DGKG with ab151967 a 1/200 dilution.

Western blot - Anti-DGKG antibody (AB151967)
  • WB

Unknown

Western blot - Anti-DGKG antibody (AB151967)

7.5% SDS PAGE

All lanes:

Western blot - Anti-DGKG antibody (ab151967) at 1/1000 dilution

All lanes:

H1299 lysate at 30 µg

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DGKG aa 450-700. The exact immunogen used to generate this antibody is proprietary information.

P49619

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKG also known as diacylglycerol kinase gamma is an important enzyme that phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA). This enzyme possesses a molecular mass of approximately 90 kDa. DGKG is expressed in many tissues with particularly high levels in brain and muscle. Its expression in these areas indicates a significant role in neuronal signaling and muscle function.
Biological function summary

DGKG influences various cellular processes by transforming diacylglycerol into phosphatidic acid. This enzymatic activity regulates the levels of DAG a critical second messenger involved in signal transduction. DGKG does not solely function on its own but is part of larger signaling complexes that mediate signal cascades. Through this involvement DGKG impacts cell growth differentiation and metabolism.

Pathways

DGKG plays a significant role in the phosphatidylinositol signaling pathway. This pathway is essential for transmitting extracellular signals to intracellular responses primarily involving protein kinase C (PKC). DGKG also interacts with proteins such as RAS and RAF kinases indicating its participation in the MAPK/ERK pathway. These interactions highlight its contribution to transmitting signals that regulate cellular proliferation and differentiation.

DGKG shows associations with cancer and neurological disorders. The altered expression or function of DGKG may contribute to oncogenesis by affecting cell signaling pathways that control cell growth. Additionally its role in neuronal signaling links it to neurological conditions possibly through interactions with proteins such as PKC. DGKG's dysregulation in these contexts suggests its potential as a therapeutic target in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 8034597). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (By similarity). Has no apparent specificity with regard to the acyl compositions of diacylglycerol (PubMed : 8034597). Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination (By similarity).
See full target information DGKG
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 12:142 PubMed39217365

2024

Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Taniguchi-Ponciano,Silvia Hinojosa-Alvarez,Jesus Hernandez-Perez,Rocio A Chavez-Santoscoy,Ilan Remba-Shapiro,Gerardo Guinto,Erika Magallon-Gayon,Benjamin Telles-Ramirez,Rodrigo Ponce de Leon-Conconi,Sandra Vela-Patiño,Sergio Andonegui-Elguera,Amayrani Cano-Zaragoza,Florencia Martinez-Mendoza,Jacobo Kerbel,Marco Loza-Mejia,Juan Rodrigo-Salazar,Alonso Mendez-Perez,Cristina Aguilar-Flores,Antonieta Chavez-Gonzalez,Elenka Ortiz-Reyes,Erick Gomez-Apo,Laura C Bonifaz,Daniel Marrero-Rodriguez,Moises Mercado

Cancer cell 42:1352-1369.e13 PubMed39029464

2024

Mammalian SWI/SNF complex activity regulates POU2F3 and constitutes a targetable dependency in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Duplaquet,Kevin So,Alexander W Ying,Shreoshi Pal Choudhuri,Xinyue Li,Grace D Xu,Yixiang Li,Xintao Qiu,Rong Li,Shilpa Singh,Xiaoli S Wu,Seth Hamilton,Victor D Chien,Qi Liu,Jun Qi,Tim D D Somerville,Hillary M Heiling,Emanuele Mazzola,Yenarae Lee,Thomas Zoller,Christopher R Vakoc,John G Doench,William C Forrester,Tinya Abrams,Henry W Long,Matthew J Niederst,Benjamin J Drapkin,Cigall Kadoch,Matthew G Oser

British journal of pharmacology 176:2736-2749 PubMed31032885

2019

Inhibitors of DAG metabolism suppress CCR2 signalling in human monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Priscilla Day,Lisa Burrows,David Richards,Samuel J Fountain
View all publications
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