Rabbit Polyclonal DGKK antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride
Liquid
Polyclonal
IHC-P | ICC/IF | |
---|---|---|
Human | Tested | Expected |
African green monkey | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/100 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:16210324, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).
Diacylglycerol kinase kappa, DAG kinase kappa, DGK-kappa, 142 kDa diacylglycerol kinase, Diglyceride kinase kappa, DGKK
Rabbit Polyclonal DGKK antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
ab111042 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Blue Ice
+4°C
-20°C
Stable for 12 months at -20°C
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This supplementary information is collated from multiple sources and compiled automatically.
The protein diacylglycerol kinase kappa (DGKK) plays a role in the phosphorylation of diacylglycerol to produce phosphatidic acid. This reaction is important in lipid signaling pathways. DGKK has a molecular mass of roughly 135 kDa. It is expressed in various tissues including the brain heart and placenta suggesting that it has functions in diverse physiological processes.
DGKK functions in the regulation of lipid second messengers which are critical for cellular signaling. It is not known to be part of a specific protein complex but it interacts with other signaling molecules to modulate cellular responses. These interactions suggest DGKK has roles in signaling cascades that control a number of cellular mechanisms.
DGKK participates in the glycerolipid metabolism pathway and the signaling events controlled by protein kinase C (PKC). These pathways involve intricate networks of interactions that influence broader cellular functions like cell growth differentiation and apoptosis. DGKK's relationship with PKC is important as both proteins coordinate to regulate the levels of diacylglycerol an important signaling lipid.
DGKK has been implicated in the development of X-linked chondrodysplasia punctata (CDPX2). This disorder which affects bone growth may arise due to disruptions in lipid signaling and metabolism. Additionally research suggests possible associations with neurological disorders though specific connections and mechanisms remain under investigation. Mahajan et al. studied the potential links between DGKK and these conditions providing insights into its roles.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ab111042 at 1/100 dilution staining DGKK in COS7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
ab111042 at 1/50 dilution staining DGKK in paraffin-embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.
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