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AB111042

Anti-DGKK antibody

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(2 Publications)

Rabbit Polyclonal DGKK antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DGKK aa 1200 to C-terminus.

View Alternative Names

Diacylglycerol kinase kappa, DAG kinase kappa, DGK-kappa, 142 kDa diacylglycerol kinase, Diglyceride kinase kappa, DGKK

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKK antibody (AB111042)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKK antibody (AB111042)

ab111042 at 1/50 dilution staining DGKK in paraffin-embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-DGKK antibody (AB111042)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DGKK antibody (AB111042)

ab111042 at 1/100 dilution staining DGKK in COS7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human DGKK aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5KSL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111042 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein diacylglycerol kinase kappa (DGKK) plays a role in the phosphorylation of diacylglycerol to produce phosphatidic acid. This reaction is important in lipid signaling pathways. DGKK has a molecular mass of roughly 135 kDa. It is expressed in various tissues including the brain heart and placenta suggesting that it has functions in diverse physiological processes.
Biological function summary

DGKK functions in the regulation of lipid second messengers which are critical for cellular signaling. It is not known to be part of a specific protein complex but it interacts with other signaling molecules to modulate cellular responses. These interactions suggest DGKK has roles in signaling cascades that control a number of cellular mechanisms.

Pathways

DGKK participates in the glycerolipid metabolism pathway and the signaling events controlled by protein kinase C (PKC). These pathways involve intricate networks of interactions that influence broader cellular functions like cell growth differentiation and apoptosis. DGKK's relationship with PKC is important as both proteins coordinate to regulate the levels of diacylglycerol an important signaling lipid.

DGKK has been implicated in the development of X-linked chondrodysplasia punctata (CDPX2). This disorder which affects bone growth may arise due to disruptions in lipid signaling and metabolism. Additionally research suggests possible associations with neurological disorders though specific connections and mechanisms remain under investigation. Mahajan et al. studied the potential links between DGKK and these conditions providing insights into its roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 16210324, PubMed : 23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).
See full target information DGKK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 18:1348478 PubMed38449737

2024

EZH2 inhibition reactivates epigenetically silenced and normalizes molecular and electrophysiological abnormalities in fragile X syndrome neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Minggang Fang,Sara K Deibler,Pranathi Meda Krishnamurthy,Feng Wang,Paola Rodriguez,Shahid Banday,Ching-Man Virbasius,Miguel Sena-Esteves,Jonathan K Watts,Michael R Green

Viruses 15: PubMed36851534

2023

Obesity Induces an Impaired Placental Antiviral Immune Response in Pregnant Women Infected with Zika Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Cláudia Calvielli Castelo Branco,Emily Araujo De Oliveira,Nátalli Zanete Pereira,Ricardo Wesley Alberca,Amaro Nunes Duarte-Neto,Luiz Fernando Ferraz Da Silva,Fernanda Guedes Luiz,Naiura Vieira Pereira,Mirian Nacagami Sotto,Naiara Naiana Dejani,Patrícia Helen Carvalho Rondó,Elyzabeth Avvad-Portari,Zilton Farias Meira De Vasconcelos,Alberto José da Silva Duarte,Tamiris Azamor,Maria Notomi Sato
View all publications
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Product promise

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