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Rabbit Polyclonal DGKK antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-DGKK antibody (AB111042), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKK antibody (AB111042), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5KSL6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Expected
African green monkey
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

African green monkey

Dilution info

1/100 - 1/500

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:16210324, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).

Alternative names

Recommended products

Rabbit Polyclonal DGKK antibody. Suitable for IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Diacylglycerol kinase kappa aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5KSL6
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab111042 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein diacylglycerol kinase kappa (DGKK) plays a role in the phosphorylation of diacylglycerol to produce phosphatidic acid. This reaction is important in lipid signaling pathways. DGKK has a molecular mass of roughly 135 kDa. It is expressed in various tissues including the brain heart and placenta suggesting that it has functions in diverse physiological processes.

Biological function summary

DGKK functions in the regulation of lipid second messengers which are critical for cellular signaling. It is not known to be part of a specific protein complex but it interacts with other signaling molecules to modulate cellular responses. These interactions suggest DGKK has roles in signaling cascades that control a number of cellular mechanisms.

Pathways

DGKK participates in the glycerolipid metabolism pathway and the signaling events controlled by protein kinase C (PKC). These pathways involve intricate networks of interactions that influence broader cellular functions like cell growth differentiation and apoptosis. DGKK's relationship with PKC is important as both proteins coordinate to regulate the levels of diacylglycerol an important signaling lipid.

Associated diseases and disorders

DGKK has been implicated in the development of X-linked chondrodysplasia punctata (CDPX2). This disorder which affects bone growth may arise due to disruptions in lipid signaling and metabolism. Additionally research suggests possible associations with neurological disorders though specific connections and mechanisms remain under investigation. Mahajan et al. studied the potential links between DGKK and these conditions providing insights into its roles.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-DGKK antibody (ab111042), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DGKK antibody (ab111042)

    ab111042 at 1/100 dilution staining DGKK in COS7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKK antibody (ab111042), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKK antibody (ab111042)

    ab111042 at 1/50 dilution staining DGKK in paraffin-embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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