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AB234805

Anti-DGKZ/DGK-zeta antibody

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(1 Publication)

Rabbit Polyclonal DGKZ/DGK-zeta antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DGKZ aa 1 to C-terminus.

View Alternative Names

DAGK6, DGKZ, Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-DGKZ/DGK-zeta antibody (AB234805)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DGKZ/DGK-zeta antibody (AB234805)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DGKZ/DGK-zeta (green) using ab234805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488 conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DGKZ aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13574

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Alternative Products

Primary Antibodies

AB105195

Anti-DGKZ/DGK-zeta antibody

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Primary Antibodies

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Anti-DGKZ/DGK-zeta antibody [EPR22040-72]

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Primary Antibodies

AB239792

Anti-DGKZ/DGK-zeta antibody [EPR22040-72] - BSA and Azide free

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Primary Antibodies

AB239081

Anti-DGKZ/DGK-zeta antibody [EPR22040-80]

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Primary Antibodies

AB239790

Anti-DGKZ/DGK-zeta antibody [EPR22040-80] - BSA and Azide free

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Secondary Antibodies

AB150077

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AB77799

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKZ also known as DGK-zeta or DGKζ is an enzyme that belongs to the diacylglycerol kinase family and plays an important role in lipid signaling pathways. The 'zeta' isoform of DGK sometimes noted in scientific literature as DGK ART and DGK MEXICO catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by ATP-dependent phosphorylation impacting signal transduction. DGKZ has a molecular mass of approximately 115 kDa. It shows expression in various tissues including brain heart and skeletal muscle with higher expression levels typically seen in the nervous system where it affects neurotransmitter signaling.
Biological function summary

DGKZ influences cellular processes such as growth differentiation and apoptosis by regulating DAG and PA levels. It forms part of a complex network of proteins involved in intracellular signaling cascades exerting effects on protein kinase C (PKC) activity. The regulatory function of DGKZ within cellular activity highlights its role in maintaining normal cellular homeostasis and responding to extracellular signals. Its enzymatic activity influences DAG-mediated pathways which are important for the activation and deactivation of downstream signaling proteins.

Pathways

DGKZ plays a critical role within the signaling pathways such as the phosphoinositide signaling and mTOR pathways. It serves as an important modulator of lipid signaling within the diacylglycerol- and phosphatidic acid-mediated signaling pathways impacting protein interactions and signaling mechanisms. Other proteins related through these pathways include PKC and mTOR with which DGKZ interacts to modulate functions such as cell proliferation and survival. The enzymatic conversion of DAG to PA by DGKZ actively regulates the availability of these lipid signaling molecules and therefore modulates their downstream effects.

DGKZ has associations with neurological disorders and cancer. Abnormal regulation or expression of DGKZ contributes to pathologies such as Alzheimer's disease where impaired lipid signaling affects neuronal function and survival. Additionally its dysregulation may promote cancer progression through aberrant cell signaling pathways. DGKZ interacts with proteins like PKC which play a role in these diseases making it a potential therapeutic target. Understanding its function and interactions helps offer insights into the development of targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed : 19744926, PubMed : 22108654, PubMed : 9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed : 15544348, PubMed : 19744926, PubMed : 22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed : 15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).. Isoform 1. Regulates RASGRP1 activity.. Isoform 2. Does not regulate RASGRP1 activity.
See full target information DGKZ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 19:75 PubMed33715625

2021

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Dong,Lei Zhao,Shiyang Zhang,Shijie Zhang,Hongyun Si
View all publications
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