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AB239080

Anti-DGKZ/DGK-zeta antibody [EPR22040-72]

  • 20ul selling size
  • Recombinant
  • Advanced Validation
  • RabMAb
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(5 Publications)

Rabbit Recombinant Monoclonal DGKZ/DGK-zeta antibody. Suitable for IP, mIHC, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

DAGK6, DGKZ, Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta

8 Images
Flow Cytometry (Intracellular) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilzedJurkat (human T cell leukemia cell line from peripheral blood) cell line labeling DGKZ/DGK-zeta with ab239080 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control, and an unlabeled control (Cell without incubation with primary antibody and secondary antibody, Blue).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 wasused as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on Purkinje cells of human cerebellum (PMID : 14511325) (PMID : 24119575) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on subset of immune cells in human tonsil (PMID : 24573202) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • IP

Unknown

Immunoprecipitation - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

DGKZ/DGK-zeta was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab239080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab239080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : Jurkat whole cell lysat 10 μg (input).
Lane 2 : ab239080 IP in Jurkat whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab239080 in Jurkat whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (ab239080)

Predicted band size: 124 kDa

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Multiplex immunohistochemistry - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Fluorescence multiplex immunohistochemical analysis of mouse cerebellum (formalin-fixed paraffin-embedded section).

Panel A : merged staining of anti-GABRA6 (magenta; Opal™690), anti-KCNA (green; Opal™520) and anti-DGKZ (gray; Opal™570) on mouse cerebellum.
Panel B : anti-GABRA6 staining granule cells in mouse cerebellum.
Panel C : anti-KCNA staining basket cells in mouse cerebellum.
Panel D : anti-DGKZ staining Purkinje cells in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300069 at a 1/500 dilution, ab313624 at a 1/5000 and ab239080 at a 1/5000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on neurons of rat brain (PMID : 14511325) (PMID : 24119575) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on neurons of mouse brain (PMID : 14511325) (PMID : 24119575) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)
  • WB

Supplier Data

Western blot - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (AB239080)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :
Lane 1-2 : 15 seconds.
Lane 3-8 : 3 seconds.

The lysates were not boiled to prevent the target protein from aggregation. Multiple bands are likely due to isoforms and phosphorylation. The molecular mass of human DGKZ / DGK-zeta is also predicated to be larger than that of rodents. The molecular profile/weight observed is consistent with what has been described in the literature (PMID : 12070163, PMID : 14511325, PMID : 9657393).

All lanes:

Western blot - Anti-DGKZ/DGK-zeta antibody [EPR22040-72] (ab239080) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg

Lane 3:

Human cerebellum tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse cerebellum tissue lysate at 20 µg

Lane 6:

Mouse thymus tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 124 kDa

Observed band size: 90-130 kDa

false

  • Carrier free

    Anti-DGKZ/DGK-zeta antibody [EPR22040-72] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22040-72

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, Flow Cyt (Intra), WB, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/5000", "mIHC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKZ also known as DGK-zeta or DGKζ is an enzyme that belongs to the diacylglycerol kinase family and plays an important role in lipid signaling pathways. The 'zeta' isoform of DGK sometimes noted in scientific literature as DGK ART and DGK MEXICO catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by ATP-dependent phosphorylation impacting signal transduction. DGKZ has a molecular mass of approximately 115 kDa. It shows expression in various tissues including brain heart and skeletal muscle with higher expression levels typically seen in the nervous system where it affects neurotransmitter signaling.
Biological function summary

DGKZ influences cellular processes such as growth differentiation and apoptosis by regulating DAG and PA levels. It forms part of a complex network of proteins involved in intracellular signaling cascades exerting effects on protein kinase C (PKC) activity. The regulatory function of DGKZ within cellular activity highlights its role in maintaining normal cellular homeostasis and responding to extracellular signals. Its enzymatic activity influences DAG-mediated pathways which are important for the activation and deactivation of downstream signaling proteins.

Pathways

DGKZ plays a critical role within the signaling pathways such as the phosphoinositide signaling and mTOR pathways. It serves as an important modulator of lipid signaling within the diacylglycerol- and phosphatidic acid-mediated signaling pathways impacting protein interactions and signaling mechanisms. Other proteins related through these pathways include PKC and mTOR with which DGKZ interacts to modulate functions such as cell proliferation and survival. The enzymatic conversion of DAG to PA by DGKZ actively regulates the availability of these lipid signaling molecules and therefore modulates their downstream effects.

DGKZ has associations with neurological disorders and cancer. Abnormal regulation or expression of DGKZ contributes to pathologies such as Alzheimer's disease where impaired lipid signaling affects neuronal function and survival. Additionally its dysregulation may promote cancer progression through aberrant cell signaling pathways. DGKZ interacts with proteins like PKC which play a role in these diseases making it a potential therapeutic target. Understanding its function and interactions helps offer insights into the development of targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed : 19744926, PubMed : 22108654, PubMed : 9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed : 15544348, PubMed : 19744926, PubMed : 22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed : 15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).. Isoform 1. Regulates RASGRP1 activity.. Isoform 2. Does not regulate RASGRP1 activity.
See full target information DGKZ
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 15:e70761 PubMed40792381

2025

DGKζ in Glycerophospholipid Metabolism Regulates the DAG and PA Balance and Interacts With PTEN to Alleviate Brain Damage in Septic Mice With Hydrogen Inhalation: A Comparative Metabolomic and Phosphoproteomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bai,Zeyu Li,Donglai Yan,Yi Jiang,Beibei Dong,Yonghao Yu

The Journal of experimental medicine 222: PubMed40227192

2025

Enhanced TLR7-dependent production of type I interferon by pDCs underlies pandemic chilblains.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Saidoune,Danyel Lee,Jeremy Di Domizio,Corentin Le Floc'h,Raphael Jenelten,Jérémie Le Pen,Vincent Bondet,Ana Joncic,Marie-Anne Morren,Vivien Béziat,Shen-Ying Zhang,Emmanuelle Jouanguy,Darragh Duffy,Charles M Rice,Curdin Conrad,Jacques Fellay,Jean-Laurent Casanova,Michel Gilliet,Ahmad Yatim

Nature immunology 24:676-689 PubMed36914891

2023

A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Lin Lo,Miriam Kuhlmann,Gabrielle Rizzuto,H Atakan Ekiz,Elizabeth M Kolawole,Monica P Revelo,Rakieb Andargachew,Zhongmei Li,Yuan-Li Tsai,Alexander Marson,Brian D Evavold,Dietmar Zehn,Arthur Weiss

Bioengineered 12:1517-1529 PubMed33926342

2021

Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Liu,Biyun Xue,Guiqin Bai,Wentao Zhang

European journal of immunology 50:1729-1745 PubMed32525220

2020

Negative control of diacylglycerol kinase ζ-mediated inhibition of T cell receptor signaling by nuclear sequestration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Danli Xie,Shimeng Zhang,Pengcheng Chen,Wenhai Deng,Yun Pan,Jinhai Xie,Jinli Wang,Bryce Liao,John W Sleasman,Xiao-Ping Zhong
View all publications
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