AB103296

Anti-DHCR7 antibody

Name: Anti-DHCR7 antibody (ab103296) | Abcam
Brand: Abcam Limited
SKU: AB103296

Be the first to review this product! Submit a review

(10 Publications)

Rabbit Polyclonal DHCR7 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHCR7 aa 1-300.

View Alternative Names

D7SR, DHCR7, 7-dehydrocholesterol reductase, 7-DHC reductase, Delta7-sterol reductase, Sterol Delta(7)-reductase, Sterol reductase SR-2

3 Images

Unknown

Western blot - Anti-DHCR7 antibody (AB103296)

All lanes:

Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution

All lanes:

Rat brain tissue lysate at 50 µg

Predicted band size: 131 kDa,44 kDa,46 kDa,51 kDa,54 kDa,56 kDa,57 kDa,60 kDa,61 kDa,65 kDa,67 kDa,70 kDa,74 kDa

Observed band size: 58 kDa,65 kDa,74 kDa,75 kDa

false

Unknown

Western blot - Anti-DHCR7 antibody (AB103296)

All lanes:

Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution

All lanes:

HepG2 cell lysate at 50 µg

Predicted band size: 54 kDa

false

Unknown

Western blot - Anti-DHCR7 antibody (AB103296)

All lanes:

Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution

All lanes:

Mouse testis tissue lysate at 50 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human DHCR7 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UBM7

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

7-dehydrocholesterol reductase (DHCR7) is an enzyme that plays an important role in cholesterol biosynthesis. It catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol in the final step of the process. DHCR7 is also known by the name sterol Δ7-reductase. The enzyme has a molecular mass of approximately 54 kDa. Expression of DHCR7 is prominent in tissues that require active cholesterol synthesis such as the liver adrenal glands and brain.

Biological function summary

DHCR7 activity ensures the maintenance of cholesterol homeostasis in cells. The enzyme operates independently and does not form part of a larger complex focusing directly on the reduction of the double bond in the C7-8 position of 7-DHC to yield cholesterol. By regulating cholesterol levels DHCR7 supports membrane fluidity and integrity contributing to the proper function of cellular structures.

Pathways

DHCR7 is an important component of the cholesterol biosynthesis pathway which is part of the larger sterol metabolic pathway. Its function is linked with other enzymes such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) highlighting their roles in sequential steps that convert squalene into cholesterol. These interactions highlight the interdependence of DHCR7 with other sterol-processing proteins influencing both cholesterol production and regulation within the body.

DHCR7 mutations can cause conditions such as Smith-Lemli-Opitz syndrome a congenital disorder characterized by multiple physical and developmental abnormalities. This condition connects DHCR7 to defective cholesterol metabolism and accumulation of toxic 7-DHC levels. Additionally the involvement of DHCR7 in cholesterol homeostasis ties it with potential dyslipidemias where abnormal lipid levels may influence cardiovascular health. Changes in DHCR7 function can lead to disruptions in normal cholesterol synthesis impacting related proteins and enzymatic pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Oxidoreductase that catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core (PubMed : 25637936, PubMed : 38297129, PubMed : 38297130, PubMed : 9465114, PubMed : 9634533). Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol (By similarity). Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation (PubMed : 38297129, PubMed : 38297130).. Component of the microsomal antiestrogen binding site (AEBS), a multiproteic complex at the ER membrane that consists of an association between cholestenol Delta-isomerase/EBP and DHCR7 (PubMed : 15175332, PubMed : 20615952). This complex is responsible for cholesterol-5,6-epoxide hydrolase (ChEH) activity, which consists in the hydration of cholesterol-5,6-epoxides (5,6-EC) into cholestane-3beta,5alpha,6beta-triol (CT) (PubMed : 20615952). The precise role of each component of this complex has not been described yet (PubMed : 20615952).

See full target information DHCR7

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1585727 PubMed40735323

2025

Lactylation prognostic signature identifies DHCR7 as a modulator of chemoresistance and immunotherapy efficacy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanqiao Zhao,Zhuo Xing,Yongqi Zhao,Haozhe Xu,Ruilin Liu,Tiejun Yang,Yinhuai Wang,Xuan Zhu

Cell communication and signaling : CCS 23:315 PubMed40598214

2025

Lycorine hydrochloride inhibits cholangiocarcinoma through cholesterol biosynthesis and PTPN11 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyun Zhao,Shuxian Peng,Liming Zou,Mingtian Zhong,Yanni Huang,Ping Wang,Mingfang Ji,Xiaodong Ma,Fugui Li

eLife 12: PubMed37490042

2023

Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Keshav Thapa,James J Kadiri,Karla Saukkonen,Iida Pennanen,Bishwa Ghimire,Minying Cai,Eriika Savontaus,Petteri Rinne

Physiological reports 9:e14671 PubMed33403800

2021

RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Azar,Mark C Valentine,Julie Trausch-Azar,Lisa Rois,Moe Mahjoub,D Michael Nelson,Alan L Schwartz

The Journal of cell biology 220: PubMed33284321

2020

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Findakly,Vikas Daggubati,Galo Garcia,Sydney A LaStella,Abrar Choudhury,Cecilia Tran,Amy Li,Pakteema Tong,Jason Q Garcia,Natasha Puri,Jeremy F Reiter,Libin Xu,David R Raleigh

Nature medicine 23:1055-1062 PubMed28805822

2017

Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pingzhao Zhang,Dejie Wang,Yu Zhao,Shancheng Ren,Kun Gao,Zhenqing Ye,Shangqian Wang,Chun-Wu Pan,Yasheng Zhu,Yuqian Yan,Yinhui Yang,Di Wu,Yundong He,Jun Zhang,Daru Lu,Xiuping Liu,Long Yu,Shimin Zhao,Yao Li,Dong Lin,Yuzhuo Wang,Liguo Wang,Yu Chen,Yinghao Sun,Chenji Wang,Haojie Huang

Nature methods 13:770-6 PubMed27479327

2016

Revealing disease-associated pathways by network integration of untargeted metabolomics.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Pirhaji,Pamela Milani,Mathias Leidl,Timothy Curran,Julian Avila-Pacheco,Clary B Clish,Forest M White,Alan Saghatelian,Ernest Fraenkel

PloS one 9:e94460 PubMed24718268

2014

Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.

Applications

IF, WB

Species

Human, Human

Kazuo Sugiyama,Hirotoshi Ebinuma,Nobuhiro Nakamoto,Noriko Sakasegawa,Yuko Murakami,Po-Sung Chu,Shingo Usui,Yuka Ishibashi,Yuko Wakayama,Nobuhito Taniki,Hiroko Murata,Yoshimasa Saito,Masayoshi Fukasawa,Kyoko Saito,Yoshiyuki Yamagishi,Takaji Wakita,Hiroshi Takaku,Toshifumi Hibi,Hidetsugu Saito,Takanori Kanai

The Journal of biological chemistry 289:4244-61 PubMed24338480

2013

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

Applications

Species

Human

Lee G D Fryer,Bethan Jones,Emma J Duncan,Claire E Hutchison,Tozen Ozkan,Paul A Williams,Olivia Alder,Max Nieuwdorp,Anna K Townley,Arjen R Mensenkamp,David J Stephens,Geesje M Dallinga-Thie,Carol C Shoulders

PloS one 8:e68011 PubMed23844146

2013

The role of oxidative stress in nervous system aging.

Applications

Species

Unspecified reactive species

Catrina Sims-Robinson,Junguk Hur,John M Hayes,Jacqueline R Dauch,Peter J Keller,Susan V Brooks,Eva L Feldman

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHCR7 antibody

Western blot - Anti-DHCR7 antibody (AB103296)

Western blot - Anti-DHCR7 antibody (AB103296)

Western blot - Anti-DHCR7 antibody (AB103296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise