Rabbit Polyclonal DHCR7 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHCR7 aa 1-300.
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- Physiological reports 9:e146712021RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Azar,Mark C Valentine,Julie Trausch-Azar,Lisa Rois,Moe Mahjoub,D Michael Nelson,Alan L SchwartzPubMed 33403800
- The Journal of cell biology 220:2020Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Findakly,Vikas Daggubati,Galo Garcia,Sydney A LaStella,Abrar Choudhury,Cecilia Tran,Amy Li,Pakteema Tong,Jason Q Garcia,Natasha Puri,Jeremy F Reiter,Libin Xu,David R RaleighPubMed 33284321
- Nature medicine 23:1055-10622017Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPingzhao Zhang et. al.PubMed 28805822
- Nature methods 13:770-62016Revealing disease-associated pathways by network integration of untargeted metabolomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeila Pirhaji et. al.PubMed 27479327
- PloS one 9:e944602014Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.
- The Journal of biological chemistry 289:4244-612013The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.Applications:WBReactive species:HumanLee G D Fryer,Bethan Jones,Emma J Duncan,Claire E Hutchison,Tozen Ozkan,Paul A Williams,Olivia Alder,Max Nieuwdorp,Anna K Townley,Arjen R Mensenkamp,David J Stephens,Geesje M Dallinga-Thie,Carol C ShouldersPubMed 24338480
- PloS one 8:e680112013The role of oxidative stress in nervous system aging.Applications:WBReactive species:Unspecified reactive speciesCatrina Sims-Robinson et. al.PubMed 23844146
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DHCR7 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBM7
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core (PubMed:25637936, PubMed:38297129, PubMed:38297130, PubMed:9465114, PubMed:9634533). Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol (By similarity). Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation (PubMed:38297129, PubMed:38297130).
Alternative names
D7SR, DHCR7, 7-dehydrocholesterol reductase, 7-DHC reductase, Delta7-sterol reductase, Sterol Delta(7)-reductase, Sterol reductase SR-2
Recommended products
Rabbit Polyclonal DHCR7 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHCR7 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DHCR7 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBM7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
7-dehydrocholesterol reductase (DHCR7) is an enzyme that plays an important role in cholesterol biosynthesis. It catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol in the final step of the process. DHCR7 is also known by the name sterol Δ7-reductase. The enzyme has a molecular mass of approximately 54 kDa. Expression of DHCR7 is prominent in tissues that require active cholesterol synthesis such as the liver adrenal glands and brain.
- Biological function summary
DHCR7 activity ensures the maintenance of cholesterol homeostasis in cells. The enzyme operates independently and does not form part of a larger complex focusing directly on the reduction of the double bond in the C7-8 position of 7-DHC to yield cholesterol. By regulating cholesterol levels DHCR7 supports membrane fluidity and integrity contributing to the proper function of cellular structures.
- Pathways
DHCR7 is an important component of the cholesterol biosynthesis pathway which is part of the larger sterol metabolic pathway. Its function is linked with other enzymes such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) highlighting their roles in sequential steps that convert squalene into cholesterol. These interactions highlight the interdependence of DHCR7 with other sterol-processing proteins influencing both cholesterol production and regulation within the body.
- Associated diseases and disorders
DHCR7 mutations can cause conditions such as Smith-Lemli-Opitz syndrome a congenital disorder characterized by multiple physical and developmental abnormalities. This condition connects DHCR7 to defective cholesterol metabolism and accumulation of toxic 7-DHC levels. Additionally the involvement of DHCR7 in cholesterol homeostasis ties it with potential dyslipidemias where abnormal lipid levels may influence cardiovascular health. Changes in DHCR7 function can lead to disruptions in normal cholesterol synthesis impacting related proteins and enzymatic pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-DHCR7 antibody (ab103296)
All lanes: Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution
All lanes: Rat brain tissue lysate at 50 µg
Predicted band size: 131 kDa, 44 kDa, 46 kDa, 51 kDa, 54 kDa, 56 kDa, 57 kDa, 60 kDa, 61 kDa, 65 kDa, 67 kDa, 70 kDa, 74 kDa
Observed band size: 58 kDa, 65 kDa, 74 kDa, 75 kDa
Western blot - Anti-DHCR7 antibody (ab103296)
All lanes: Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution
All lanes: Mouse testis tissue lysate at 50 µg
Predicted band size: 54 kDa
Western blot - Anti-DHCR7 antibody (ab103296)
All lanes: Western blot - Anti-DHCR7 antibody (ab103296) at 1/500 dilution
All lanes: HepG2 cell lysate at 50 µg
Predicted band size: 54 kDa
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