AB226784

Anti-DHCR7 antibody - C-terminal

Name: Anti-DHCR7 antibody - C-terminal (ab226784) | Abcam
Brand: Abcam Limited
SKU: AB226784
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DHCR7 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHCR7.

View Alternative Names

D7SR, DHCR7, 7-dehydrocholesterol reductase, 7-DHC reductase, Delta7-sterol reductase, Sterol Delta(7)-reductase, Sterol reductase SR-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHCR7 antibody - C-terminal (AB226784)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHCR7 antibody - C-terminal (AB226784)

Paraffin-embedded mouse liver tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHCR7 antibody - C-terminal (AB226784)

Paraffin-embedded rat duodenum tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DHCR7 antibody - C-terminal (AB226784)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DHCR7 antibody - C-terminal (ab226784) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, boiled at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, boiled at 30 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, unboiled at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, unboiled at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DHCR7. The exact immunogen used to generate this antibody is proprietary information.

Q9UBM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

7-dehydrocholesterol reductase (DHCR7) is an enzyme that plays an important role in cholesterol biosynthesis. It catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol in the final step of the process. DHCR7 is also known by the name sterol Δ7-reductase. The enzyme has a molecular mass of approximately 54 kDa. Expression of DHCR7 is prominent in tissues that require active cholesterol synthesis such as the liver adrenal glands and brain.

Biological function summary

DHCR7 activity ensures the maintenance of cholesterol homeostasis in cells. The enzyme operates independently and does not form part of a larger complex focusing directly on the reduction of the double bond in the C7-8 position of 7-DHC to yield cholesterol. By regulating cholesterol levels DHCR7 supports membrane fluidity and integrity contributing to the proper function of cellular structures.

Pathways

DHCR7 is an important component of the cholesterol biosynthesis pathway which is part of the larger sterol metabolic pathway. Its function is linked with other enzymes such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) highlighting their roles in sequential steps that convert squalene into cholesterol. These interactions highlight the interdependence of DHCR7 with other sterol-processing proteins influencing both cholesterol production and regulation within the body.

DHCR7 mutations can cause conditions such as Smith-Lemli-Opitz syndrome a congenital disorder characterized by multiple physical and developmental abnormalities. This condition connects DHCR7 to defective cholesterol metabolism and accumulation of toxic 7-DHC levels. Additionally the involvement of DHCR7 in cholesterol homeostasis ties it with potential dyslipidemias where abnormal lipid levels may influence cardiovascular health. Changes in DHCR7 function can lead to disruptions in normal cholesterol synthesis impacting related proteins and enzymatic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core (PubMed : 25637936, PubMed : 38297129, PubMed : 38297130, PubMed : 9465114, PubMed : 9634533). Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol (By similarity). Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation (PubMed : 38297129, PubMed : 38297130).

See full target information DHCR7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109774 PubMed38711443

2024

Phospholipase PLA2G7 is complementary to GPX4 in mitigating punicic-acid-induced ferroptosis in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Vermonden,Manon Martin,Katarzyna Glowacka,Ineke Neefs,Josef Ecker,Marcus Höring,Gerhard Liebisch,Cathy Debier,Olivier Feron,Yvan Larondelle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHCR7 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHCR7 antibody - C-terminal (AB226784)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHCR7 antibody - C-terminal (AB226784)

Western blot - Anti-DHCR7 antibody - C-terminal (AB226784)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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