AB270453

Anti-Dhh antibody [EPR23386-212]

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DHH antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Desert hedgehog protein, DHH, HHG-3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (AB270453)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (AB270453)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Dhh with ab270453 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human testis (PMID : 22132805). The section was incubated with ab270453 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Lab

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

All lanes:

Recombinant Human Desert Hedgehog/Dhh protein (aa 23-198) 20ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 19 kDa

false

Lab

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature. Band at 19kDa could be DHH N-terminal fragment (PMID : 31018998).

The expression profile observed is consistent with what has been described in the literature(PMID : 22132805).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 19 kDa

false

Supplier Data

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Blocking buffer and concentration : 5% NFDM/TBST . Diluting buffer and concentration : 5% NFDM/TBST. Negative control : colon (PMID : 22132805). ab181602 was used as a loading control.

All lanes:

Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysat at 40 µg

Lane 2:

Mouse colon tissue lysate at 40 µg

Lane 3:

Rat testis tissue lysate at 40 µg

Lane 4:

Rat colon tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 19 kDa

false

Exposure time: 80s

Carrier free

Anti-Dhh antibody [EPR23386-212] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23386-212

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'Desert Hedgehog' (Dhh) also known as DHH weighs approximately 42 kDa and plays an important role in signaling pathways. Dhh functions mechanically by sending signals that control cell growth and differentiation during embryonic development. This protein expresses in specific tissues such as the gonads and is especially associated with Sertoli cells in the testes.

Biological function summary

Dhh contributes to the regulation of morphogenesis and tissue patterning. Dhh forms part of the Hedgehog signaling pathway a critical signaling complex needed for the communication between cells. This interaction is important for cellular processes such as growth survival and differentiation. The protein is critical in development and is significant for the proper formation of various organs and tissues.

Pathways

Its involvement with the Hedgehog signaling pathway is essential. This pathway impacts the development of several structures and organs by regulating gene expression. Other proteins involved in this pathway include Patched1 (PTCH1) and Smoothened (SMO). Pathway deregulation can lead to issues and is a significant area of study in several types of biological research.

Dhh has links to conditions like gonadal dysgenesis and peripheral neuropathy. Abnormal function or expression of the Dhh protein can lead to developmental defects or contribute toward disease progression. The interaction with other proteins such as PTCH1 further highlights its involvement in the pathogenesis of these conditions. Understanding Dhh’s role offers potential insights into therapeutic interventions for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Desert hedgehog protein. The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (DHH-N) (By similarity). Both activities occur in the endoplasmic reticulum (By similarity). Once cleaved, the C-product is degraded in the endoplasmic reticulum (By similarity). Functions in cell-cell mediated juxtacrine signaling (PubMed : 24342078). Promotes endothelium integrity (PubMed : 33063110). Binds to PTCH1 receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes in endothelial cells (PubMed : 33063110). In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube (By similarity).. Desert hedgehog protein N-product. The dually lipidated desert hedgehog protein N-product is essential for a variety of patterning events during development (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed : 11472839, PubMed : 33063110). Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules (By similarity). Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism (By similarity). May induce motor neurons in lateral neural tube and may have a polarizing activity (PubMed : 11472839). Prevents the desert hedgehog protein precursor binding to PTCH1 (PubMed : 33063110).

See full target information DHH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of immunopathology and pharmacology 37:3946320231208833 PubMed37864517

2023

PKNOX1 acts as a transcription factor of DHH and promotes the progression of stomach adenocarcinoma by regulating the Hedgehog signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Siwei Zhao,Liqing Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dhh antibody [EPR23386-212]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (AB270453)

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise