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Rabbit Recombinant Monoclonal DHH antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453), expandable thumbnail
  • Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (AB270453), expandable thumbnail
  • Western blot - Anti-Dhh antibody [EPR23386-212] (AB270453), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Desert hedgehog protein. The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Functions in cell-cell mediated juxtacrine signaling (PubMed:24342078). Promotes endothelium integrity (PubMed:33063110). Binds to PTCH1 receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes in endothelial cells (PubMed:33063110). In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube (By similarity). Desert hedgehog protein N-product. The dually lipidated desert hedgehog protein N-product is essential for a variety of patterning events during development (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed:11472839, PubMed:33063110). Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules (By similarity). Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism (By similarity). May induce motor neurons in lateral neural tube and may have a polarizing activity (PubMed:11472839). Prevents the desert hedgehog protein precursor binding to PTCH1 (PubMed:33063110).

Alternative names

Desert hedgehog protein, DHH, HHG-3

Recommended products

Rabbit Recombinant Monoclonal DHH antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23386-212
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein 'Desert Hedgehog' (Dhh) also known as DHH weighs approximately 42 kDa and plays an important role in signaling pathways. Dhh functions mechanically by sending signals that control cell growth and differentiation during embryonic development. This protein expresses in specific tissues such as the gonads and is especially associated with Sertoli cells in the testes.

Biological function summary

Dhh contributes to the regulation of morphogenesis and tissue patterning. Dhh forms part of the Hedgehog signaling pathway a critical signaling complex needed for the communication between cells. This interaction is important for cellular processes such as growth survival and differentiation. The protein is critical in development and is significant for the proper formation of various organs and tissues.

Pathways

Its involvement with the Hedgehog signaling pathway is essential. This pathway impacts the development of several structures and organs by regulating gene expression. Other proteins involved in this pathway include Patched1 (PTCH1) and Smoothened (SMO). Pathway deregulation can lead to issues and is a significant area of study in several types of biological research.

Associated diseases and disorders

Dhh has links to conditions like gonadal dysgenesis and peripheral neuropathy. Abnormal function or expression of the Dhh protein can lead to developmental defects or contribute toward disease progression. The interaction with other proteins such as PTCH1 further highlights its involvement in the pathogenesis of these conditions. Understanding Dhh’s role offers potential insights into therapeutic interventions for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453), expandable thumbnail

    Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

    All lanes: Recombinant Human Desert Hedgehog/Dhh protein (aa 23-198) 20ng

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa

    Observed band size: 19 kDa

  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453), expandable thumbnail

    Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature. Band at 19kDa could be DHH N-terminal fragment (PMID: 31018998).

    The expression profile observed is consistent with what has been described in the literature(PMID:22132805).

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human colon tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 44 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Dhh with ab270453 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human testis (PMID: 22132805). The section was incubated with ab270453 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453), expandable thumbnail

    Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)

    Blocking buffer and concentration: 5% NFDM/TBST .

    Diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: colon (PMID: 22132805).

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.

    All lanes: Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysat at 40 µg

    Lane 2: Mouse colon tissue lysate at 40 µg

    Lane 3: Rat testis tissue lysate at 40 µg

    Lane 4: Rat colon tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 44 kDa

    Observed band size: 19 kDa

    Exposure time: 80s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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