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AB324655

Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Desert hedgehog protein, DHH, HHG-3, Dhh

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Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)

Interpolated concentration of native Dhh was measured at different sample concentrations. Undiluted sample is as follows : TM4 cell extract (1 mg/mL) and mouse testis tissue extract (0.5 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)

Interpolated concentration of native Dhh was measured at different sample concentrations. Undiluted sample is as follows : mouse urine 50%. Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DHH antibody [EPR29306-39] - BSA and Azide free (Capture) (AB324655)

Sandwich ELISA using mouse Dhh immunogen protein standard curve with the capture antibody ab324655 at 2 µg/mL and detector antibody ab324656 at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29306-39

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The pair is specific to mouse DHH and does not cross-react with IHH or SHH.

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Reactivity data

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Product details

ab324655 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse DHH. The recommended pair for sandwich ELISA is:

Capture: ab324655, Mouse DHH Capture Antibody (unconjugated)

Detector: ab324656, Mouse DHH Detector Antibody (unconjugated)

The reference range value is 312.5-20000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Desert hedgehog protein. The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (DHH-N) (By similarity). Both activities occur in the endoplasmic reticulum (By similarity). Once cleaved, the C-product is degraded in the endoplasmic reticulum (By similarity). Functions in cell-cell mediated juxtacrine signaling (By similarity). Promotes endothelium integrity (PubMed : 33063110). Binds to PTCH1 receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes in endothelial cells (PubMed : 33063110). In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube (PubMed : 10482238).. Desert hedgehog protein N-product. The dually lipidated desert hedgehog protein N-product (DHH-Np) is essential for a variety of patterning events during development (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed : 9811851). Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules (PubMed : 11090455, PubMed : 8805249). Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism (PubMed : 32151560). May induce motor neurons in lateral neural tube and may have a polarizing activity. Prevents the desert hedgehog protein precursor binding to PTCH1 (By similarity). Binds PTCH2 (PubMed : 9811851).
See full target information Dhh
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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