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AB237688

Anti-DHHC-12 antibody

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(1 Publication)

Rabbit Polyclonal DHHC-12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC12 aa 100-200.

View Alternative Names

ZNF400, PSEC0008, ZDHHC12, Palmitoyltransferase ZDHHC12, DHHC domain-containing cysteine-rich protein 12, Zinc finger DHHC domain-containing protein 12, Zinc finger protein 400, DHHC-12

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-DHHC-12 antibody (AB237688)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHHC-12 antibody (AB237688)

MCF7 (Human breast adenocarcinoma cell line) cells stained for DHHC-12 (Green) using ab237688 at 1/100 dilution in iCC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-12 antibody (AB237688)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-12 antibody (AB237688)

Paraffin-embedded human thyroid gland tissue stained for DHHC-12 using ab237688 at 1/100 dilution in immunohistochemical analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-12 antibody (AB237688)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-12 antibody (AB237688)

Paraffin-embedded human kidney tissue stained for DHHC-12 using ab237688 at 1/100 dilution in immunohistochemical analysis

Western blot - Anti-DHHC-12 antibody (AB237688)
  • WB

Supplier Data

Western blot - Anti-DHHC-12 antibody (AB237688)

All lanes:

Western blot - Anti-DHHC-12 antibody (ab237688) at 1/500 dilution

All lanes:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZDHHC12 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q96GR4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DHHC-12 protein also known as zDHHC12 functions as a palmitoyl acyltransferase enzyme facilitating the addition of palmitate to target proteins. This post-translational modification of proteins affects their trafficking stability or function. DHHC-12 has a molecular mass of approximately 39 kDa. Expression of DHHC-12 occurs in various tissues with significant levels detectable in brain and some other organs. Its activity frequently involves dynamic regulation of protein interactions and membrane associations.
Biological function summary

DHHC-12 is key in ensuring regulation of protein localization and signaling pathways by mediating protein palmitoylation. As part of a large family of palmitoyl acyltransferases it functions based on substrate specificity displaying selective enzymatic activity towards a subset of protein targets. This selectivity allows DHHC-12 to influence diverse cellular processes including signal transduction and protein trafficking. While not directly part of a multiprotein complex DHHC-12 interacts with various proteins during its palmitoylation activities.

Pathways

DHHC-12 plays important roles in cellular signaling and membrane protein trafficking. It significantly contributes to the Ras signaling pathway which controls cell proliferation differentiation and survival. Through this pathway DHHC-12 interacts with Ras proteins modulating their membrane association and activity. DHHC-12 also influences the Wnt signaling pathway which affects cell fate and tissue homeostasis. It ensures proper membrane localization of Wnt pathway components maintaining the signaling cascade integrity.

DHHC-12 has implications in neurodegenerative conditions with particular relevance to Alzheimer's disease. Altered palmitoylation patterns mediated by DHHC-12 can affect amyloid precursor protein processing or tau protein function linking it to disease pathogenesis. Additionally aberrant DHHC-12 expression or activity correlates with certain types of cancers where it affects important oncogenic pathways. Through these disease associations DHHC-12 connects to proteins such as APP in Alzheimer's disease and oncogenes in cancer highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed : 36586411). Has a palmitoyltransferase activity toward gephyrin/GPHN, regulating its clustering at synapses and its function in gamma-aminobutyric acid receptor clustering (By similarity). Thereby, indirectly regulates GABAergic synaptic transmission (By similarity). Acts as an inhibitor of the NLRP3 inflammasome by mediating palmitoylation of NLRP3, thereby promoting NLRP3 degradation by the chaperone-mediated autophagy (CMA) process (PubMed : 36586411).
See full target information ZDHHC12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:4968-4980 PubMed36056909

2022

Risk stratification and prognosis prediction based on inflammation-related gene signature in lung squamous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhai,Si Chen,Fangfang Duan,Junye Wang,Zerui Zhao,Yaobin Lin,Bingyu Rao,Yizhi Wang,Lie Zheng,Hao Long
View all publications
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Product promise

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