Rabbit Polyclonal DHHC-12 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC12 aa 100-200.
View Alternative Names
ZNF400, PSEC0008, ZDHHC12, Palmitoyltransferase ZDHHC12, DHHC domain-containing cysteine-rich protein 12, Zinc finger DHHC domain-containing protein 12, Zinc finger protein 400, DHHC-12
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-12 antibody (AB237688)
Immunohistochemical analysis of formalin fixed paraffin embedded human kidney tissue labelling DHHC-12 with ab237688 at a 1 : 50 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab237688 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB.
- WB
Supplier Data
Western blot - Anti-DHHC-12 antibody (AB237688)
All lanes:
Western blot - Anti-DHHC-12 antibody (ab237688) at 1/1000 dilution
Lane 1:
MCF7 whole cell lysate at 30 µg
Lane 2:
COLO205 whole cell lysate at 30 µg
Lane 3:
HEK293 whole cell lysate at 30 µg
Lane 4:
Jurkat whole cell lysate at 30 µg
Lane 5:
U251 whole cell lysate at 30 µg
Lane 6:
Mouse brain tissue lysate at 30 µg
Lane 7:
Rat brain tissue lysate at 30 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG at 1/40000 dilution
Observed band size: 37 kDa
false
Exposure time: 60s
Complementary Products
Reactivity data
Properties and storage information
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
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Aliquoting information
Storage information
Supplementary information
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Biological function summary
DHHC-12 is key in ensuring regulation of protein localization and signaling pathways by mediating protein palmitoylation. As part of a large family of palmitoyl acyltransferases it functions based on substrate specificity displaying selective enzymatic activity towards a subset of protein targets. This selectivity allows DHHC-12 to influence diverse cellular processes including signal transduction and protein trafficking. While not directly part of a multiprotein complex DHHC-12 interacts with various proteins during its palmitoylation activities.
Pathways
DHHC-12 plays important roles in cellular signaling and membrane protein trafficking. It significantly contributes to the Ras signaling pathway which controls cell proliferation differentiation and survival. Through this pathway DHHC-12 interacts with Ras proteins modulating their membrane association and activity. DHHC-12 also influences the Wnt signaling pathway which affects cell fate and tissue homeostasis. It ensures proper membrane localization of Wnt pathway components maintaining the signaling cascade integrity.
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Target data
Publications (1)
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Cancer medicine 12:4968-4980 PubMed36056909
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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