JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121203

Anti-DHHC-15 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal DHHC-15 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC15 aa 200 to C-terminus.

View Alternative Names

UNQ1969/PRO4501, ZDHHC15, Palmitoyltransferase ZDHHC15, Acyltransferase ZDHHC15, Zinc finger DHHC domain-containing protein 15, DHHC-15

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-15 antibody (AB121203)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-15 antibody (AB121203)

ab121203 at 1/5 dilution staining DHHC-15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-DHHC-15 antibody (AB121203)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DHHC-15 antibody (AB121203)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-DHHC-15 antibody (AB121203)
  • WB

Unknown

Western blot - Anti-DHHC-15 antibody (AB121203)

All lanes:

Western blot - Anti-DHHC-15 antibody (ab121203) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

A431 cell lysate

Lane 4:

Human Liver cell lysate

Lane 5:

Human tonsil cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZDHHC15 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96MV8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/250", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHHC-15 also known as ZDHHC15 is a member of the DHHC domain-containing protein family. This protein functions as a palmitoyltransferase catalyzing the addition of palmitate a 16-carbon fatty acid to specific cysteine residues in substrate proteins. It exhibits an approximate molecular mass of 37-40 kDa. Researchers have found DHHC-15 predominantly in the brain but it is not limited to this organ as it also expresses in other tissues like the heart and lungs. This suggests its diverse role in cellular activities across different tissues.
Biological function summary

DHHC family members contribute to the regulation of protein trafficking stability and function. They often function as subunits within larger protein complexes influencing signaling pathways by modifying protein-membrane associations. DHHC-15 precisely modifies proteins that are important for neurotransmission. By doing so DHHC-15 influences synaptic signaling and plasticity impacting how neurons communicate and adapt in the central nervous system. Its palmitoylation activity ensures proper localization and function of target proteins enhancing cellular communication.

Pathways

DHHC-15 influences protein palmitoylation pathways which are essential in post-translational modifications that affect protein targeting and function. The protein is intimately involved in the Ras signaling pathway which is essential for cell growth and differentiation. Proteins such as H-Ras and N-Ras undergo palmitoylation by DHHC-15 which in turn regulates their membrane association and signaling activity. These interactions highlight the importance of DHHC-15 in modulating key intracellular signaling routes and cellular responses.

Alterations in DHHC-15 have correlations with neurodegenerative diseases and intellectual disabilities. Modified DHHC-15 expression or function might contribute to the pathology of Alzheimer's disease by affecting the processing or localization of Alzheimer-related proteins. Additionally mutations or dysfunctions in DHHC-15 have associations with intellectual disability disorders possibly linked to its role in modifying proteins important for brain development and function. Its interactions with APP (amyloid precursor protein) further underline its significance in disease pathology providing a potential therapeutic target in these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed : 18817523, PubMed : 23034182). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (By similarity). Palmitoylates IGF2R and SORT1, promoting their partitioning to an endosomal membrane subdomain where they can interact with the retromer cargo-selective complex (PubMed : 18817523). Thereby, regulates retrograde transport from endosomes to the Golgi apparatus of these lysosomal sorting receptors and plays a role in trafficking of lysosomal proteins (PubMed : 18817523). In the nervous system, catalyzes the palmitoylation of DLG4/PSD95 and regulates its synaptic clustering and function in synaptogenesis (By similarity). Could be involved in the differentiation of dopaminergic neurons and the development of the diencephalon (By similarity). Could also catalyze the palmitoylation of GAP43 (By similarity). Could also palmitoylate DNAJC5 and regulate its localization to the Golgi membrane (By similarity). Could also palmitoylate FYN as shown in vitro (PubMed : 19956733). May palmitoylate CALHM3 subunit of gustatory voltage-gated ion channels and modulate channel gating and kinetics.
See full target information ZDHHC15
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37039765

2023

Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Danya Abazari,Angela R Wild,Tian Qiu,Bryan C Dickinson,Shernaz X Bamji

Journal of cell science 132: PubMed31189538

2019

Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.

Applications

Unspecified application

Species

Unspecified reactive species

Bhavin S Shah,Jordan J Shimell,Shernaz X Bamji
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB154790

Anti-DHHC-18 antibody [EPR9871]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (AB154790)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB235369

Anti-ZDHHC4/DC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com