Rabbit Recombinant Monoclonal DHHC-18 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Western blot - Anti-DHHC-18 antibody [EPR9871] (AB154790), expandable thumbnail](https://content.abcam.com/products/images/dhhc-18-antibody-epr9871-ab154790--western-blot-img130782.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DHHC-18 antibody [EPR9871] (AB154790)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (AB154790), expandable thumbnail](https://content.abcam.com/products/images/dhhc-18-antibody-epr9871-ab154790--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (AB154790)")
Publications
Filter by
- Journal of cell science 136:2023Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanya Abazari,Angela R Wild,Tian Qiu,Bryan C Dickinson,Shernaz X BamjiPubMed 37039765
- Journal of experimental & clinical cancer research 38:252019DHHC protein family targets different subsets of glioma stem cells in specific niches.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueran Chen et. al.PubMed 30658672
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as CGAS, HRAS and LCK (PubMed:23034182, PubMed:27481942, PubMed:35438208). Acts as a negative regulator of the cGAS-STING pathway be mediating palmitoylation and inactivation of CGAS (PubMed:35438208). May also have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and therefore regulate G protein-coupled receptor signaling (PubMed:27481942).
Alternative names
Palmitoyltransferase ZDHHC18, DHHC domain-containing cysteine-rich protein 18, Zinc finger DHHC domain-containing protein 18, DHHC-18, ZDHHC18
Recommended products
Rabbit Recombinant Monoclonal DHHC-18 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9871
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DHHC-18 also known as ZDHHC18 functions as a palmitoyltransferase enzyme responsible for the addition of palmitate a type of lipid to target proteins. This process is known as palmitoylation and it influences the proteins' membrane association and function. DHHC-18 has a molecular weight of about 41 kDa. Expression of DHHC-18 occurs in various tissues including the brain and heart implicating its role in diverse cellular processes.
- Biological function summary
DHHC-18 contributes to protein trafficking and signaling by influencing the membrane dynamics. It does not act alone but as part of a large dynamic network of proteins regulating lipid modifications. This network helps in processes such as synaptic signaling and neuronal growth highlighting its importance in neurobiology. Through its catalytic activity DHHC-18 can modify substrates like Ras-related proteins affecting their functional states and interactions.
- Pathways
DHHC-18 interacts within the Ras signaling and synaptic transmission pathways. Through its involvement in Ras signaling DHHC-18 modulates the activity of Ras proteins which contributes to cell growth and differentiation. Furthermore the synaptic transmission pathway gets influenced by this palmitoylation enzyme affecting neurotransmitter release with interacting proteins like PSD-95 playing key roles in maintaining synaptic plasticity and stability.
- Associated diseases and disorders
DHHC-18 has implications in neurological conditions such as schizophrenia and autism spectrum disorders. Its direct connection involves the modulation of neuronal membrane proteins important for proper brain function. Abnormal palmitoylation due to DHHC-18 dysfunction can disrupt the balance of synaptic signaling leading to cognitive and behavioral anomalies. Dysregulation can also influence proteins like NLGN3 and CNTNAP2 which are pivotal in psychiatric and neurodevelopmental disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-DHHC-18 antibody [EPR9871] (ab154790), expandable thumbnail](https://content.abcam.com/products/images/dhhc-18-antibody-epr9871-ab154790--western-blot-img130782.jpg "Western blot - Anti-DHHC-18 antibody [EPR9871] (ab154790)")
Western blot - Anti-DHHC-18 antibody [EPR9871] (ab154790)
All lanes: Western blot - Anti-DHHC-18 antibody [EPR9871] (ab154790) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Fetal heart lysate at 10 µg
Lane 3: Human bladder lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (ab154790), expandable thumbnail](https://content.abcam.com/products/images/dhhc-18-antibody-epr9871-ab154790--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92416.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (ab154790)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (ab154790)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DHHC-18 with ab154790 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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