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AB154790

Anti-DHHC-18 antibody [EPR9871]

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(2 Publications)

Rabbit Recombinant Monoclonal DHHC-18 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Palmitoyltransferase ZDHHC18, DHHC domain-containing cysteine-rich protein 18, Zinc finger DHHC domain-containing protein 18, DHHC-18, ZDHHC18

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (AB154790)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-18 antibody [EPR9871] (AB154790)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DHHC-18 with ab154790 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DHHC-18 antibody [EPR9871] (AB154790)
  • WB

Unknown

Western blot - Anti-DHHC-18 antibody [EPR9871] (AB154790)

All lanes:

Western blot - Anti-DHHC-18 antibody [EPR9871] (ab154790) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Lane 3:

Human bladder lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 42 kDa

false

  • Carrier free

    Anti-DHHC-18 antibody [EPR9871] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9871

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-15 antibody (AB121203)

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHHC-18 also known as ZDHHC18 functions as a palmitoyltransferase enzyme responsible for the addition of palmitate a type of lipid to target proteins. This process is known as palmitoylation and it influences the proteins' membrane association and function. DHHC-18 has a molecular weight of about 41 kDa. Expression of DHHC-18 occurs in various tissues including the brain and heart implicating its role in diverse cellular processes.
Biological function summary

DHHC-18 contributes to protein trafficking and signaling by influencing the membrane dynamics. It does not act alone but as part of a large dynamic network of proteins regulating lipid modifications. This network helps in processes such as synaptic signaling and neuronal growth highlighting its importance in neurobiology. Through its catalytic activity DHHC-18 can modify substrates like Ras-related proteins affecting their functional states and interactions.

Pathways

DHHC-18 interacts within the Ras signaling and synaptic transmission pathways. Through its involvement in Ras signaling DHHC-18 modulates the activity of Ras proteins which contributes to cell growth and differentiation. Furthermore the synaptic transmission pathway gets influenced by this palmitoylation enzyme affecting neurotransmitter release with interacting proteins like PSD-95 playing key roles in maintaining synaptic plasticity and stability.

DHHC-18 has implications in neurological conditions such as schizophrenia and autism spectrum disorders. Its direct connection involves the modulation of neuronal membrane proteins important for proper brain function. Abnormal palmitoylation due to DHHC-18 dysfunction can disrupt the balance of synaptic signaling leading to cognitive and behavioral anomalies. Dysregulation can also influence proteins like NLGN3 and CNTNAP2 which are pivotal in psychiatric and neurodevelopmental disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as CGAS, HRAS and LCK (PubMed : 23034182, PubMed : 27481942, PubMed : 35438208). Acts as a negative regulator of the cGAS-STING pathway be mediating palmitoylation and inactivation of CGAS (PubMed : 35438208). May also have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and therefore regulate G protein-coupled receptor signaling (PubMed : 27481942).
See full target information ZDHHC18
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37039765

2023

Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Danya Abazari,Angela R Wild,Tian Qiu,Bryan C Dickinson,Shernaz X Bamji

Journal of experimental & clinical cancer research 38:25 PubMed30658672

2019

DHHC protein family targets different subsets of glioma stem cells in specific niches.

Applications

Unspecified application

Species

Unspecified reactive species

Xueran Chen,Lei Hu,Haoran Yang,Huihui Ma,Kaiqin Ye,Chenggang Zhao,Zhiyang Zhao,Haiming Dai,Hongzhi Wang,Zhiyou Fang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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