AB200572

Anti-DHHC-5 antibody

Name: Anti-DHHC-5 antibody (ab200572) | Abcam
Brand: Abcam Limited
SKU: AB200572
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal DHHC-5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZDHHC5 aa 500-550.

View Alternative Names

KIAA1748, ZNF375, ZDHHC5, Palmitoyltransferase ZDHHC5, Zinc finger DHHC domain-containing protein 5, Zinc finger protein 375, DHHC-5

3 Images

Supplier Data

Immunoprecipitation - Anti-DHHC-5 antibody (AB200572)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling DHHC-5 using ab200572 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated DHHC-5, ab200572 was used at 0.4 μg/ml.

All lanes:

Immunoprecipitation - Anti-DHHC-5 antibody (ab200572)

Predicted band size: 78 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-DHHC-5 antibody (AB200572)

All lanes:

Western blot - Anti-DHHC-5 antibody (ab200572) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 50 µg

Lane 3:

Jurkat whole cell lysates at 50 µg

Predicted band size: 78 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-DHHC-5 antibody (AB200572)

All lanes:

Western blot - Anti-DHHC-5 antibody (ab200572) at 0.1 µg/mL

Lane 1:

TCMK-1 whole cell lysate at 50 µg

Lane 2:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 78 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ZDHHC5 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q9C0B5

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHHC-5 also known as zDHHC5 or DHHC5 is a palmitoyltransferase enzyme located primarily in the plasma membrane of cells. It plays an important mechanical role by catalyzing the addition of palmitate to proteins through thioester linkage which is important for regulating protein localization stability and function. DHHC-5 has a molecular weight of approximately 48 kDa. It expresses widely in various tissues with prominent expression in the brain heart and lungs indicating its significance in multiple physiological systems.

Biological function summary

DHHC-5 contributes to the modulation of signal transduction and cellular communication. This protein functions as part of a complex with its substrate proteins where it transfers lipid groups to them influencing their interaction with other molecules and membranes. By doing so DHHC-5 affects processes like synaptic plasticity cell adhesion and intracellular trafficking. Its role in these mechanisms suggests it is a versatile player in cellular network dynamics.

Pathways

DHHC-5 is integral to the palmitoylation pathway which regulates protein trafficking and signaling. Within this framework it influences critical biological pathways such as the Wnt signaling pathway impacting cell fate determination. Additionally DHHC-5 interacts with other palmitoyltransferases and proteins such as G-proteins contributing to their localization and function by palmitoylating them and therefore playing a role in various signaling networks.

DHHC-5 has been associated with neurological disorders such as schizophrenia and neurodegenerative diseases like Alzheimer's disease. Its altered function and expression can disrupt normal protein localization and signaling leading to pathophysiological states. In Alzheimer's disease DHHC-5 may affect proteins involved in amyloid precursor protein processing influencing the accumulation of beta-amyloid plaques. Understanding the connections between DHHC-5 and these disorders aids in comprehending its potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, GSDMD, NLRP3, NOD1, NOD2, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, inflammation, fatty acid uptake, bacterial sensing or cardiac functions (PubMed : 21820437, PubMed : 29185452, PubMed : 31402609, PubMed : 31649195, PubMed : 34293401, PubMed : 38092000, PubMed : 38530158, PubMed : 38599239). Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) (PubMed : 26334723). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins (PubMed : 26334723). Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2 (PubMed : 26334723). Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2 (PubMed : 31402609). Plays a role in innate immune response by mediating the palmitoylation of NOD1 and NOD2 and their proper recruitment to the bacterial entry site and phagosomes (PubMed : 31649195, PubMed : 34293401). Participates also in fatty acid uptake by palmitoylating CD36 and thereby targeting it to the plasma membrane (PubMed : 32958780). Upon binding of fatty acids to CD36, gets phosphorylated by LYN leading to inactivation and subsequent CD36 caveolar endocytosis (PubMed : 32958780). Controls oligodendrocyte development by catalyzing STAT3 palmitoylation (By similarity). Acts as a regulator of inflammatory response by mediating palmitoylation of NLRP3 and GSDMD (PubMed : 38092000, PubMed : 38530158, PubMed : 38599239). Palmitoylates NLRP3 to promote inflammasome assembly and activation (PubMed : 38092000). Activates pyroptosis by catalyzing palmitoylation of gasdermin-D (GSDMD), thereby promoting membrane translocation and pore formation of GSDMD (PubMed : 38530158, PubMed : 38599239).

See full target information ZDHHC5

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:1026 PubMed36476988

2022

HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Ting Zeng,Tian-Hao Deng,Zhen Liu,Jia-Rong Zhan,Yuan-Zhen Ma,Yuan-Yuan Yan,Xiao Sun,Ying-Hui Zhu,Yan Li,Xin-Yuan Guan,Lei Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHHC-5 antibody

Immunoprecipitation - Anti-DHHC-5 antibody (AB200572)

Western blot - Anti-DHHC-5 antibody (AB200572)

Western blot - Anti-DHHC-5 antibody (AB200572)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise