JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224093

Anti-DHHC-5 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal DHHC-5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC5 aa 450-650.

View Alternative Names

KIAA1748, ZNF375, ZDHHC5, Palmitoyltransferase ZDHHC5, Zinc finger DHHC domain-containing protein 5, Zinc finger protein 375, DHHC-5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-DHHC-5 antibody (AB224093)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHHC-5 antibody (AB224093)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for DHHC-5 (green) using ab224093 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)

Paraffin-embedded human placenta tissue stained for DHHC-5 using ab224093 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)

Paraffin-embedded human esophagus tissue stained for DHHC-5 using ab224093 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-5 antibody (AB224093)

Paraffin-embedded human dunodenum tissue stained for DHHC-5 using ab224093 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZDHHC5 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.

Q9C0B5

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHHC-5 also known as zDHHC5 or DHHC5 is a palmitoyltransferase enzyme located primarily in the plasma membrane of cells. It plays an important mechanical role by catalyzing the addition of palmitate to proteins through thioester linkage which is important for regulating protein localization stability and function. DHHC-5 has a molecular weight of approximately 48 kDa. It expresses widely in various tissues with prominent expression in the brain heart and lungs indicating its significance in multiple physiological systems.
Biological function summary

DHHC-5 contributes to the modulation of signal transduction and cellular communication. This protein functions as part of a complex with its substrate proteins where it transfers lipid groups to them influencing their interaction with other molecules and membranes. By doing so DHHC-5 affects processes like synaptic plasticity cell adhesion and intracellular trafficking. Its role in these mechanisms suggests it is a versatile player in cellular network dynamics.

Pathways

DHHC-5 is integral to the palmitoylation pathway which regulates protein trafficking and signaling. Within this framework it influences critical biological pathways such as the Wnt signaling pathway impacting cell fate determination. Additionally DHHC-5 interacts with other palmitoyltransferases and proteins such as G-proteins contributing to their localization and function by palmitoylating them and therefore playing a role in various signaling networks.

DHHC-5 has been associated with neurological disorders such as schizophrenia and neurodegenerative diseases like Alzheimer's disease. Its altered function and expression can disrupt normal protein localization and signaling leading to pathophysiological states. In Alzheimer's disease DHHC-5 may affect proteins involved in amyloid precursor protein processing influencing the accumulation of beta-amyloid plaques. Understanding the connections between DHHC-5 and these disorders aids in comprehending its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, GSDMD, NLRP3, NOD1, NOD2, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, inflammation, fatty acid uptake, bacterial sensing or cardiac functions (PubMed : 21820437, PubMed : 29185452, PubMed : 31402609, PubMed : 31649195, PubMed : 34293401, PubMed : 38092000, PubMed : 38530158, PubMed : 38599239). Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) (PubMed : 26334723). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins (PubMed : 26334723). Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2 (PubMed : 26334723). Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2 (PubMed : 31402609). Plays a role in innate immune response by mediating the palmitoylation of NOD1 and NOD2 and their proper recruitment to the bacterial entry site and phagosomes (PubMed : 31649195, PubMed : 34293401). Participates also in fatty acid uptake by palmitoylating CD36 and thereby targeting it to the plasma membrane (PubMed : 32958780). Upon binding of fatty acids to CD36, gets phosphorylated by LYN leading to inactivation and subsequent CD36 caveolar endocytosis (PubMed : 32958780). Controls oligodendrocyte development by catalyzing STAT3 palmitoylation (By similarity). Acts as a regulator of inflammatory response by mediating palmitoylation of NLRP3 and GSDMD (PubMed : 38092000, PubMed : 38530158, PubMed : 38599239). Palmitoylates NLRP3 to promote inflammasome assembly and activation (PubMed : 38092000). Activates pyroptosis by catalyzing palmitoylation of gasdermin-D (GSDMD), thereby promoting membrane translocation and pore formation of GSDMD (PubMed : 38530158, PubMed : 38599239).
See full target information ZDHHC5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu5091 PubMed40203102

2025

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Felix Kraus,João P L Coelho,Muskaan Vasandani,Jiuchun Zhang,Benjamin M Adams,Joao A Paulo,J Wade Harper,Sichen Shao
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB200572

Anti-DHHC-5 antibody

Western blot - Anti-DHHC-5 antibody (AB200572)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB235369

Anti-ZDHHC4/DC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

  • 0
  • 0
Primary Antibodies

AB237688

Anti-DHHC-12 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DHHC-12 antibody (AB237688)

  • 0
  • 0
Primary Antibodies

AB121203

Anti-DHHC-15 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHHC-15 antibody (AB121203)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com