AB174288

Anti-DHODH antibody [EPR11814]

RabMAb
Recombinant
20ul selling size
What is this?

Be the first to review this product! Submit a review

(11 Publications)

Rabbit Recombinant Monoclonal DHODH antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

DHOdehase, Dihydroorotate oxidase, DHODH

2 Images

Supplier Data

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

Note : ab174288 is diluted in 1% BSA.

All lanes:

Western blot - Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution

Lane 1:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Lane 5:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Predicted band size: 43 kDa

false

Lab

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

All lanes:

Western blot - Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Human heart lysates at 15 µg

Lane 3:

Mouse heart lysates at 15 µg

Lane 4:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11814

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dihydroorotate dehydrogenase (DHODH) also known as URA1 is an enzyme weighing approximately 43 kDa. It plays a critical role in the de novo pyrimidine biosynthesis pathway. DHODH is mainly expressed in the inner mitochondrial membrane where it catalyzes the conversion of dihydroorotate to orotate. This reaction involves ubiquinone as an electron acceptor making DHODH an important player in cellular respiration. Its functionality is essential for the synthesis of nucleotides particularly in rapidly proliferating cells.

Biological function summary

Dihydroorotate dehydrogenase is involved in establishing the pyrimidine pool necessary for DNA and RNA synthesis. It is a single-component enzyme but its activity supports the function of other enzymes in the pathway such as carbamoyl-phosphate synthetase. The proper regulation of DHODH is integral to cellular proliferation and immune responses. Cells that synthesize nucleotides at high rates such as immune cells and cancer cells heavily depend on its activity.

Pathways

Dihydroorotate dehydrogenase is an important player in the pyrimidine biosynthesis pathway and links to the mitochondrial electron transport chain. Its interaction with ubiquinone underlines its role in energy production connecting it to respiratory complexes. DHODH works alongside proteins such as orotate phosphoribosyltransferase which further transforms orotate to orotidine-5'-monophosphate in pyrimidine biosynthesis. These pathways are vital for maintaining the balance of nucleotide levels impacting processes like DNA replication.

Dihydroorotate dehydrogenase is relevant to autoimmune diseases like rheumatoid arthritis and cancers. Inhibitors such as leflunomide and brequinar target DHODH to disrupt the proliferation of rapidly dividing cells. Leflunomide which is metabolized to teriflunomide reduces immune cell turnover in rheumatoid arthritis illustrating DHODH's therapeutic importance. Similarly it is a target in cancer treatment where its inhibition can slow down tumor growth interfacing with other metabolic regulators active in malignancies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.

See full target information DHODH

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:697 PubMed41053036

2025

The deubiquitylase OTUB1 drives gemcitabine resistance in pancreatic cancer by enhancing pyrimidine metabolism through modulating DHODH mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Wenming Zhang,Rui Liu,Junwen Hu,Shuangyan Wan,Yeqin Zou,Tong Che,Jin Zhang,Leifeng Chen,Xiaogang Peng

Clinical and translational medicine 15:e70270 PubMed40088428

2025

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ouwen Li,Ke An,Han Wang,Xianbin Li,Yueqin Wang,Lan Huang,Yue Du,Nuo Qin,Jiasheng Dong,Jingyao Wei,Ranran Sun,Yong Shi,Yanjia Guo,Xiangyi Sun,Ying Yang,Yun-Gui Yang,Quancheng Kan,Xin Tian

Chemical biology & drug design 105:e70022 PubMed39757387

2025

Dexmedetomidine Inhibits Ferroptosis by Regulating the SRY-Box Transcription Factor 9/Divalent Metal Transporter-1 Axis to Alleviate Cerebral Ischemia/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Liu,Qionghua Liu,Xuekang Zhang,Gan Li

Cardiovascular diabetology 23:432 PubMed39633391

2024

PACS2/CPT1A/DHODH signaling promotes cardiomyocyte ferroptosis in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Xiang,Qi Lyu,Shuhua Chen,Jie Ouyang,Di Xiao,Quanjun Liu,HaiJiao Long,Xinru Zheng,Xiaoping Yang,Hongwei Lu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404753 PubMed39303219

2024

Mitochondrial Dysfunction-Evoked DHODH Acetylation is Involved in Renal Cell Ferroptosis during Cisplatin-Induced Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Nan Liang,Yue-Yue Guo,Xiao-Yi Zhang,Ya-Hui Ren,Yi-Zhang He,Zhi-Bing Liu,De-Xiang Xu,Shen Xu

BMC cancer 24:761 PubMed38918775

2024

Targeted inhibition of DHODH is synergistic with BCL2 blockade in HGBCL with concurrent MYC and BCL2 rearrangement.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Liu,Wenbin Mo,Miao Chen,Yi Qu,Pingping Wang,Ying Liang,Xiaojing Yan

Nature cell biology 26:811-824 PubMed38671262

2024

LPCAT1-mediated membrane phospholipid remodelling promotes ferroptosis evasion and tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwen Li,Yameng Hu,Haiqing Zheng,Man Li,Yuanji Liu,Rongni Feng,Xincheng Li,Shuxia Zhang,Miaoling Tang,Meisongzhu Yang,Ruyuan Yu,Yingru Xu,Xinyi Liao,Suwen Chen,Wanying Qian,Qiliang Zhang,Daolin Tang,Bo Li,Libing Song,Jun Li

American journal of cancer research 13:5236-5253 PubMed38058815

2023

Risk model-guided identification of MTDH expression as a marker for ferroptosis induction therapy in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Wang,Yuzhang Gao,Rui Miao,Lisha Li,Abiyasi Nanding,Yong Liu,Xin Zhang

Redox biology 62:102708 PubMed37116254

2023

17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Tian,Yuan Xie,Zaixin Guo,Penghui Feng,Yang You,Qi Yu

Journal of ovarian research 15:19 PubMed35105347

2022

High expression of TRIM24 predicts worse prognosis and promotes proliferation and metastasis of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Zhang,Hong Chen,Baijuan Ding,Wei Jiang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHODH antibody [EPR11814]

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Product promise