AB190266

Anti-DHPS/DHS antibody

Name: Anti-DHPS/DHS antibody (ab190266) | Abcam
Brand: Abcam Limited
SKU: AB190266
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal DHPS/DHS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DHPS aa 300 to C-terminus.

View Alternative Names

DS, DHPS, Deoxyhypusine synthase, DHS

2 Images

Supplier Data

Immunoprecipitation - Anti-DHPS/DHS antibody (AB190266)

Immunoprecipitation of Hela whole cell lysates (0.5-1 mg per IP reaction; 20% of IP loaded) labeling DHPS/DHS with ab190266 at 1 μg/ml by western blot. DHPS/DHS was immunoprecipitated using ab190266 (lane 1) or control IgG (lane 2).

All lanes:

Immunoprecipitation - Anti-DHPS/DHS antibody (ab190266)

Predicted band size: 41 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-DHPS/DHS antibody (AB190266)

All lanes:

Western blot - Anti-DHPS/DHS antibody (ab190266) at 1 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK whole cell lysate at 50 µg

Lane 5:

NIH-3T3 whole cell lysate at 50 µg

Predicted band size: 41 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DHPS aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49366

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dihydropteroate synthase (DHPS) also known as dihydropteroate synthase (synonymously notated as 'DHPS/DHS') is an enzyme that catalyzes the condensation of para-aminobenzoic acid (pABA) with pterin pyrophosphate to form dihydropteroate an important intermediate in folate biosynthesis. It possesses a molecular mass of approximately 30 kDa. This enzyme is known to be expressed in a diverse range of prokaryotic organisms certain lower eukaryotes and plants playing an important role in the folate biosynthesis pathways essential for cellular processes.

Biological function summary

Dihydropteroate synthase serves a fundamental role in the synthesis of folic acid a critical cofactor in the transfer of one-carbon units in cellular metabolism. DHPS functions within the larger multi-enzyme complex of folate synthesis. The enzyme's activity directly influences the production of tetrahydrofolate (THF) which is critical in nucleic acid and amino acid biosynthesis thereby impacting cell growth and replication in these organisms.

Pathways

DHPS plays an essential role in the folate biosynthesis and subsequent nucleotide biosynthesis pathways. The DHPS enzyme's activity directly affects the folate cycle by producing dihydropteroate a precursor to tetrahydrofolate. Proteins such as dihydrofolate reductase (DHFR) are closely related within these biological pathways facilitating the eventual reduction of dihydrofolate to THF therefore enabling critical biosynthetic reactions necessary for cell viability.

DHPS activity is notably implicated in bacterial resistance to sulfonamide antibiotics which target and inhibit DHPS function to disrupt folate synthesis. This enzyme's presence and alteration are associated with infections caused by sulfonamide-resistant bacteria including Streptococcus pneumoniae and Plasmodium falciparum. In these instances the protein interacts or associates with enzymes like DHFR which can likewise mutate to confer resistance complicating treatment strategies and necessitating the development of novel therapeutic approaches to target resistant strains effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue (PubMed : 30661771). This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.

See full target information DHPS

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 633:895-904 PubMed39169180

2024

Short-term post-fast refeeding enhances intestinal stemness via polyamines.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Imada,Saleh Khawaled,Heaji Shin,Sven W Meckelmann,Charles A Whittaker,Renan Oliveira Corrêa,Chiara Alquati,Yixin Lu,Guodong Tie,Dikshant Pradhan,Gizem Calibasi-Kocal,Luiza Martins Nascentes Melo,Gabriele Allies,Jonas Rösler,Pia Wittenhofer,Jonathan Krystkiewicz,Oliver J Schmitz,Jatin Roper,Marco Aurelio Ramirez Vinolo,Luigi Ricciardiello,Evan C Lien,Matthew G Vander Heiden,Ramesh A Shivdasani,Chia-Wei Cheng,Alpaslan Tasdogan,Ömer H Yilmaz

Life science alliance 6: PubMed36725334

2023

A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Essletzbichler,Vitaly Sedlyarov,Fabian Frommelt,Didier Soulat,Leonhard X Heinz,Adrijana Stefanovic,Benedikt Neumayer,Giulio Superti-Furga

Cell 184:4186-4202.e20 PubMed34216540

2021

Polyamine metabolism is a central determinant of helper T cell lineage fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Puleston,Francesc Baixauli,David E Sanin,Joy Edwards-Hicks,Matteo Villa,Agnieszka M Kabat,Marcin M Kamiński,Michal Stanckzak,Hauke J Weiss,Katarzyna M Grzes,Klara Piletic,Cameron S Field,Mauro Corrado,Fabian Haessler,Chao Wang,Yaarub Musa,Lena Schimmelpfennig,Lea Flachsmann,Gerhard Mittler,Nir Yosef,Vijay K Kuchroo,Joerg M Buescher,Stefan Balabanov,Edward J Pearce,Douglas R Green,Erika L Pearce

Molecular cell 76:110-125.e9 PubMed31474573

2019

Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Hanlin Zhang,Ghada Alsaleh,Jack Feltham,Yizhe Sun,Gennaro Napolitano,Thomas Riffelmacher,Philip Charles,Lisa Frau,Philip Hublitz,Zhanru Yu,Shabaz Mohammed,Andrea Ballabio,Stefan Balabanov,Jane Mellor,Anna Katharina Simon

Cell metabolism 30:352-363.e8 PubMed31130465

2019

Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Puleston,Michael D Buck,Ramon I Klein Geltink,Ryan L Kyle,George Caputa,David O'Sullivan,Alanna M Cameron,Angela Castoldi,Yaarub Musa,Agnieszka M Kabat,Ying Zhang,Lea J Flachsmann,Cameron S Field,Annette E Patterson,Stefanie Scherer,Francesca Alfei,Francesc Baixauli,S Kyle Austin,Beth Kelly,Mai Matsushita,Jonathan D Curtis,Katarzyna M Grzes,Matteo Villa,Mauro Corrado,David E Sanin,Jing Qiu,Nora Pällman,Katelyn Paz,Maria Elena Maccari,Bruce R Blazar,Gerhard Mittler,Joerg M Buescher,Dietmar Zehn,Sabine Rospert,Edward J Pearce,Stefan Balabanov,Erika L Pearce

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHPS/DHS antibody

Immunoprecipitation - Anti-DHPS/DHS antibody (AB190266)

Western blot - Anti-DHPS/DHS antibody (AB190266)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise