Rabbit Polyclonal DHPS/DHS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DHPS aa 300 to C-terminus.
Images
Publications
Filter by
- Life science alliance 6:2023A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick Essletzbichler,Vitaly Sedlyarov,Fabian Frommelt,Didier Soulat,Leonhard X Heinz,Adrijana Stefanovic,Benedikt Neumayer,Giulio Superti-FurgaPubMed 36725334
- Cell 184:4186-4202.e202021Polyamine metabolism is a central determinant of helper T cell lineage fidelity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Puleston,Francesc Baixauli,David E Sanin,Joy Edwards-Hicks,Matteo Villa,Agnieszka M Kabat,Marcin M Kamiński,Michal Stanckzak,Hauke J Weiss,Katarzyna M Grzes,Klara Piletic,Cameron S Field,Mauro Corrado,Fabian Haessler,Chao Wang,Yaarub Musa,Lena Schimmelpfennig,Lea Flachsmann,Gerhard Mittler,Nir Yosef,Vijay K Kuchroo,Joerg M Buescher,Stefan Balabanov,Edward J Pearce,Douglas R Green,Erika L PearcePubMed 34216540
- Molecular cell 76:110-125.e92019Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanlin Zhang et. al.PubMed 31474573
- Cell metabolism 30:352-363.e82019Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Puleston et. al.PubMed 31130465
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DHPS aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49366
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue (PubMed:30661771). This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
Alternative names
DS, DHPS, Deoxyhypusine synthase, DHS
Recommended products
Rabbit Polyclonal DHPS/DHS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DHPS aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DHPS aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49366
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The dihydropteroate synthase (DHPS) also known as dihydropteroate synthase (synonymously notated as 'DHPS/DHS') is an enzyme that catalyzes the condensation of para-aminobenzoic acid (pABA) with pterin pyrophosphate to form dihydropteroate an important intermediate in folate biosynthesis. It possesses a molecular mass of approximately 30 kDa. This enzyme is known to be expressed in a diverse range of prokaryotic organisms certain lower eukaryotes and plants playing an important role in the folate biosynthesis pathways essential for cellular processes.
- Biological function summary
Dihydropteroate synthase serves a fundamental role in the synthesis of folic acid a critical cofactor in the transfer of one-carbon units in cellular metabolism. DHPS functions within the larger multi-enzyme complex of folate synthesis. The enzyme's activity directly influences the production of tetrahydrofolate (THF) which is critical in nucleic acid and amino acid biosynthesis thereby impacting cell growth and replication in these organisms.
- Pathways
DHPS plays an essential role in the folate biosynthesis and subsequent nucleotide biosynthesis pathways. The DHPS enzyme's activity directly affects the folate cycle by producing dihydropteroate a precursor to tetrahydrofolate. Proteins such as dihydrofolate reductase (DHFR) are closely related within these biological pathways facilitating the eventual reduction of dihydrofolate to THF therefore enabling critical biosynthetic reactions necessary for cell viability.
- Associated diseases and disorders
DHPS activity is notably implicated in bacterial resistance to sulfonamide antibiotics which target and inhibit DHPS function to disrupt folate synthesis. This enzyme's presence and alteration are associated with infections caused by sulfonamide-resistant bacteria including Streptococcus pneumoniae and Plasmodium falciparum. In these instances the protein interacts or associates with enzymes like DHFR which can likewise mutate to confer resistance complicating treatment strategies and necessitating the development of novel therapeutic approaches to target resistant strains effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunoprecipitation - Anti-DHPS/DHS antibody (ab190266)
Immunoprecipitation of Hela whole cell lysates (0.5-1 mg per IP reaction; 20% of IP loaded) labeling DHPS/DHS with ab190266 at 1 μg/ml by western blot. DHPS/DHS was immunoprecipitated using ab190266 (lane 1) or control IgG (lane 2).
All lanes: Immunoprecipitation - Anti-DHPS/DHS antibody (ab190266)
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3min
Western blot - Anti-DHPS/DHS antibody (ab190266)
All lanes: Western blot - Anti-DHPS/DHS antibody (ab190266) at 1 µg/mL
Lane 1: Hela whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK whole cell lysate at 50 µg
Lane 5: NIH-3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com