Rabbit Polyclonal DHPS/DHS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHPS aa 1-150.
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Publications
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- Nature communications 13:77962022Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8 T lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas C J Tan,Van Kelly,Xiaoyan Zou,David Wright,Tony Ly,Rose ZamoyskaPubMed 36528626
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DHPS aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P49366
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue (PubMed:30661771). This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
Alternative names
DS, DHPS, Deoxyhypusine synthase, DHS
Recommended products
Rabbit Polyclonal DHPS/DHS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHPS aa 1-150.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DHPS aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P49366
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The dihydropteroate synthase (DHPS) also known as dihydropteroate synthase (synonymously notated as 'DHPS/DHS') is an enzyme that catalyzes the condensation of para-aminobenzoic acid (pABA) with pterin pyrophosphate to form dihydropteroate an important intermediate in folate biosynthesis. It possesses a molecular mass of approximately 30 kDa. This enzyme is known to be expressed in a diverse range of prokaryotic organisms certain lower eukaryotes and plants playing an important role in the folate biosynthesis pathways essential for cellular processes.
- Biological function summary
Dihydropteroate synthase serves a fundamental role in the synthesis of folic acid a critical cofactor in the transfer of one-carbon units in cellular metabolism. DHPS functions within the larger multi-enzyme complex of folate synthesis. The enzyme's activity directly influences the production of tetrahydrofolate (THF) which is critical in nucleic acid and amino acid biosynthesis thereby impacting cell growth and replication in these organisms.
- Pathways
DHPS plays an essential role in the folate biosynthesis and subsequent nucleotide biosynthesis pathways. The DHPS enzyme's activity directly affects the folate cycle by producing dihydropteroate a precursor to tetrahydrofolate. Proteins such as dihydrofolate reductase (DHFR) are closely related within these biological pathways facilitating the eventual reduction of dihydrofolate to THF therefore enabling critical biosynthetic reactions necessary for cell viability.
- Associated diseases and disorders
DHPS activity is notably implicated in bacterial resistance to sulfonamide antibiotics which target and inhibit DHPS function to disrupt folate synthesis. This enzyme's presence and alteration are associated with infections caused by sulfonamide-resistant bacteria including Streptococcus pneumoniae and Plasmodium falciparum. In these instances the protein interacts or associates with enzymes like DHFR which can likewise mutate to confer resistance complicating treatment strategies and necessitating the development of novel therapeutic approaches to target resistant strains effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (ab224134)
Paraffin-embedded human colon tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (ab224134)
Paraffin-embedded human prostate tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (ab224134)
Paraffin-embedded human fallopian tube tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-DHPS/DHS antibody (ab224134)
All lanes: Western blot - Anti-DHPS/DHS antibody (ab224134) at 1/100 dilution
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: DHPS/DHS overexpressing HEK-393T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Developed using the ECL technique.
Predicted band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DHPS/DHS antibody (ab224134)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DHPS/DHS (green) using ab224134 at 4 μg/ml in ICC/IF.
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