AB224134

Anti-DHPS/DHS antibody

Name: Anti-DHPS/DHS antibody (ab224134) | Abcam
Brand: Abcam Limited
SKU: AB224134
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DHPS/DHS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHPS aa 1-150.

View Alternative Names

DS, DHPS, Deoxyhypusine synthase, DHS

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Paraffin-embedded human colon tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Paraffin-embedded human prostate tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Paraffin-embedded human fallopian tube tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DHPS/DHS antibody (AB224134)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHPS/DHS antibody (AB224134)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DHPS/DHS (green) using ab224134 at 4 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-DHPS/DHS antibody (AB224134)

All lanes:

Western blot - Anti-DHPS/DHS antibody (ab224134) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

DHPS/DHS overexpressing HEK-393T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DHPS aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P49366

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dihydropteroate synthase (DHPS) also known as dihydropteroate synthase (synonymously notated as 'DHPS/DHS') is an enzyme that catalyzes the condensation of para-aminobenzoic acid (pABA) with pterin pyrophosphate to form dihydropteroate an important intermediate in folate biosynthesis. It possesses a molecular mass of approximately 30 kDa. This enzyme is known to be expressed in a diverse range of prokaryotic organisms certain lower eukaryotes and plants playing an important role in the folate biosynthesis pathways essential for cellular processes.

Biological function summary

Dihydropteroate synthase serves a fundamental role in the synthesis of folic acid a critical cofactor in the transfer of one-carbon units in cellular metabolism. DHPS functions within the larger multi-enzyme complex of folate synthesis. The enzyme's activity directly influences the production of tetrahydrofolate (THF) which is critical in nucleic acid and amino acid biosynthesis thereby impacting cell growth and replication in these organisms.

Pathways

DHPS plays an essential role in the folate biosynthesis and subsequent nucleotide biosynthesis pathways. The DHPS enzyme's activity directly affects the folate cycle by producing dihydropteroate a precursor to tetrahydrofolate. Proteins such as dihydrofolate reductase (DHFR) are closely related within these biological pathways facilitating the eventual reduction of dihydrofolate to THF therefore enabling critical biosynthetic reactions necessary for cell viability.

DHPS activity is notably implicated in bacterial resistance to sulfonamide antibiotics which target and inhibit DHPS function to disrupt folate synthesis. This enzyme's presence and alteration are associated with infections caused by sulfonamide-resistant bacteria including Streptococcus pneumoniae and Plasmodium falciparum. In these instances the protein interacts or associates with enzymes like DHFR which can likewise mutate to confer resistance complicating treatment strategies and necessitating the development of novel therapeutic approaches to target resistant strains effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue (PubMed : 30661771). This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.

See full target information DHPS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7796 PubMed36528626

2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8 T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas C J Tan,Van Kelly,Xiaoyan Zou,David Wright,Tony Ly,Rose Zamoyska

Oncogene 40:3136-3151 PubMed33824473

2021

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyi Lao,Xiaozhen Zhang,Tao Ma,Jian Xu,Hanshen Yang,Yi Duan,Honggang Ying,Xiaoyu Zhang,Chengxiang Guo,Junyu Qiu,Xueli Bai,Tingbo Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHPS/DHS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHPS/DHS antibody (AB224134)

Immunocytochemistry/ Immunofluorescence - Anti-DHPS/DHS antibody (AB224134)

Western blot - Anti-DHPS/DHS antibody (AB224134)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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