JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220483

Anti-DHRS2 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal DHRS2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHRS2 aa 50-200.

View Alternative Names

SDR25C1, DHRS2, Dicarbonyl reductase HEP27, Protein D, Short chain dehydrogenase/reductase family 25C member 1, Protein SDR25C1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DHRS2 antibody (AB220483)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHRS2 antibody (AB220483)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized RT-4 cells labeling DHRS2 with ab220483 at 4 μg/ml (green).

Western blot - Anti-DHRS2 antibody (AB220483)
  • WB

Supplier Data

Western blot - Anti-DHRS2 antibody (AB220483)

All lanes:

Western blot - Anti-DHRS2 antibody (ab220483) at 1/250 dilution

All lanes:

RT-4 cell lysate

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DHRS2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q13268

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHRS2 also known as dehydrogenase/reductase SDR family member 2 is a 28-kilodalton protein involved in oxidoreductase activity. This protein catalyzes the conversion of carbonyl compounds to alcohols using NAD(P)H as a cofactor. DHRS2 is expressed in various tissues with significant levels in the liver and kidney which are key sites for metabolic processes. This wide expression points to its role in metabolic regulation across different physiological systems.
Biological function summary

DHRS2 functions in the cellular metabolism of retinoids and steroids impacting cellular differentiation and proliferation. It acts as part of a complex involved in the detoxification and biosynthesis of compounds in cells. The enzyme facilitates the regulation of intracellular signaling through its action on metabolite processing thereby influencing growth and metabolic homeostasis.

Pathways

DHRS2 plays a role in retinol metabolism and steroid biosynthesis. It interacts with proteins such as retinol dehydrogenases and alcohol dehydrogenases which are important in these pathways. This involvement ensures the regulation of retinoid availability which affects visual and developmental processes and the modulation of steroid hormones which are key for maintaining metabolic functions.

DHRS2 has implications in the development of metabolic disorders and certain cancers. Changes in the expression or activity of DHRS2 may disrupt normal metabolic homeostasis contributing to disorders such as diabetes. Furthermore in cancer DHRS2 associates with proteins like p53 where its altered activity can impact tumor suppression or progression. This suggests DHRS2 as a potential target for therapeutic interventions in these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

NADPH-dependent oxidoreductase which catalyzes the reduction of dicarbonyl compounds. Displays reductase activity in vitro with 3,4-hexanedione, 2,3-heptanedione and 1-phenyl-1,2-propanedione as substrates (PubMed : 16685466). May function as a dicarbonyl reductase in the enzymatic inactivation of reactive carbonyls involved in covalent modification of cellular components (PubMed : 16685466). Also displays a minor hydroxysteroid dehydrogenase activity toward bile acids such as ursodeoxycholic acid (UDCA) and isoursodeoxycholic acid (isoUDCA), which makes it unlikely to control hormone levels (PubMed : 16685466). Doesn't show any activity in vitro with retinoids and sugars as substrates (PubMed : 16685466). Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21 (PubMed : 20547751). Reduces proliferation, migration and invasion of cancer cells and well as the production of ROS in cancer (PubMed : 29106393).
See full target information DHRS2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 13:1336600 PubMed38282619

2024

Lysosomal trafficking regulator restricts intracellular growth of by inhibiting the expansion of -containing vacuole and upregulating expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Wan,Shan Zhang,Mingliang Zhao,Xuan OuYang,Yonghui Yu,Xiaolu Xiong,Ning Zhao,Jun Jiao

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2205395 PubMed36594618

2023

Combined Single-Cell and Spatial Transcriptomics Reveal the Metabolic Evolvement of Breast Cancer during Early Dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ming Liu,Jing-Yu Ge,Yu-Fei Chen,Tong Liu,Lie Chen,Cui-Cui Liu,Ding Ma,Yi-Yu Chen,Yu-Wen Cai,Ying-Ying Xu,Zhi-Ming Shao,Ke-Da Yu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB194801

Anti-DHRS2 antibody

Western blot - Anti-DHRS2 antibody (AB194801)

  • 0
  • 0

Complementary Products

Assay Kits

AB284009

Human KAP1 ELISA Kit

Sandwich ELISA - Human KAP1 ELISA Kit (AB284009)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB109185

Anti-MUC1 antibody [EPR1023]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (AB109185)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com