Rabbit Polyclonal DHRS4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DHRS4 aa 150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DHRS4 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTZ2
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Target data
Function
NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:18571493, PubMed:19056333). Reduces 3-ketosteroids and benzil into 3beta-hydroxysteroids and R-benzoin, respectively, in contrast to the stereoselectivity of non-primate DHRS4s which produce 3alpha-hydroxysteroids and S-benzoin (PubMed:19056333). Diplays low activity toward all-trans-retinal and no activity toward 9-cis-retinal as compared to non-primate mammals (PubMed:18571493, PubMed:19056333). In the reverse reaction, catalyze the NAD-dependent oxidation of 3beta-hydroxysteroids and alcohol, but with much lower efficiency (PubMed:18571493, PubMed:19056333). Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds (PubMed:18571493, PubMed:19056333). Isoform 7. No detected catalytic activity in vitro, possibly due to the lack of catalytic site. Isoform 8. NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones. Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds. Has a higher catalytic activity for xenobiotic alpha-dicarbonyl compounds, sucha as benzil, than isoform 1 and is involved in benzil detoxification.
Alternative names
SDR25C2, UNQ851/PRO1800, DHRS4, Dehydrogenase/reductase SDR family member 4, NADPH-dependent carbonyl reductase, NADPH-dependent retinol dehydrogenase/reductase, Peroxisomal short-chain alcohol dehydrogenase, SCAD-SRL, Short chain dehydrogenase/reductase family 25C member 2, Short-chain dehydrogenase/reductase family member 4, CR, NRDR, humNRDR, PSCD, Protein SDR25C2
Recommended products
Rabbit Polyclonal DHRS4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DHRS4 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DHRS4 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTZ2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DHRS4 also known as Dehydrogenase/Reductase SDR Family Member 4 plays an important role in metabolic processes. This enzyme with a mass of approximately 36 kDa primarily engages in oxidoreductive activities affecting the metabolism of steroids and retinoids. Its expression is particularly high in the liver but can also be found in lung and kidney tissues. The protein facilitates the conversion of aldehydes and ketones into their corresponding alcohols influencing metabolic pathways significantly.
- Biological function summary
The dehydrogenase/reductase family member 4 interacts with multiple substrates impacting cellular redox status. This protein is not known to be part of a larger complex but interacts dynamically with other molecules within the cell. It contributes notably to the synthesis and breakdown of retinoic acids which are essential for cellular differentiation and growth. By managing retinoic acid levels DHRS4 helps regulate gene expression and various cellular responses.
- Pathways
DHRS4 integrates into the retinoid metabolism and transport pathway as well as the steroid hormone biosynthesis pathway. It works alongside proteins such as retinol-binding protein 4 (RBP4) in retinoid metabolism influencing the availability and activity of retinoic acids. The interplay of DHRS4 with these pathways indicates its role in maintaining homeostasis of important biochemical processes in the body affecting energy metabolism and hormone regulation.
- Associated diseases and disorders
DHRS4 has been linked to metabolic disorders including hepatic diseases due to its role in lipid metabolism. Alterations in DHRS4 expression or function may contribute to fatty liver disease through disrupted retinoid processing. Additionally its involvement in steroid metabolism indicates possible connections to endocrine disorders occasionally interacting with cytochrome P450 enzymes which further impacts hormone synthesis and balance. The enzyme's importance in managing metabolic activities highlights its relevance in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS4 antibody (ab254702)
Paraffin-embedded human kidney tissue stained for DHRS4 using ab254702 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-DHRS4 antibody (ab254702)
Western blot analysis labeling DHRS4 with ab254702 at 0.04 ug/ml.
All lanes: Western blot - Anti-DHRS4 antibody (ab254702) at 0.04 µg/mL
Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Predicted band size: 30 kDa
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