AB249260

Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DHRS7 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DHRS7A, RETSDR4, SDR34C1, CGI-86, UNQ285/PRO3448, DHRS7, Dehydrogenase/reductase SDR family member 7, Retinal short-chain dehydrogenase/reductase 4, Short chain dehydrogenase/reductase family 34C member 1, retSDR4, Protein SDR34C1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

This data was developed using ab156021, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling DHRS7 using ab156021 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

This data was developed using ab156021, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling DHRS7 using ab156021 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

This data was developed using ab156021, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DHRS7 antibody [EPR9339(B)] (<a href='/en-us/products/primary-antibodies/dhrs7-antibody-epr9339b-ab156021'>ab156021</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9339(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249260 is the carrier-free version of ab156021.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHRS7 short for dehydrogenase/reductase 7 is an enzyme involved in oxidation-reduction reactions. This protein has a molecular mass of approximately 37 kDa. As part of the short-chain dehydrogenase/reductase family which is notable for its role in metabolizing a wide range of substrates DHRS7 is expressed in several tissues including liver kidney and brain. It contributes to the conversion of various small molecules although the specific substrates it acts on remain less completely defined.

Biological function summary

DHRS7 influences cellular processes by participating in steroid and retinoid metabolism. It operates as an independent enzyme rather than as part of a larger complex. Its activity affects the balance of oxidized and reduced states of substrates impacting cell signaling and homeostasis. The enzyme modifies key signaling molecules potentially influencing the receptor activities that these molecules regulate therefore playing a role in cellular responses and adaptation.

Pathways

DHRS7 integrates into metabolic and signaling pathways through its function in oxidation-reduction reactions. It participates in the steroid metabolism pathway where it perhaps interacts with cytochrome P450 enzymes that mediate a variety of biosynthetic processes. DHRS7 may also engage in the retinoid metabolic pathway related through the mutual need for enzymes capable of handling lipid-soluble vitamins and hormones. Its interaction with other enzymes in these pathways helps maintain cellular equilibrium and contributes to broader metabolic networks.

DHRS7 interactions have been linked with metabolic syndrome and liver disease. Abnormal activities of DHRS7 have been observed in conditions involving disrupted metabolic states suggesting a link to liver disease through impaired processing of substrates in the liver. The altered function of this enzyme can also affect pathways involving other proteins like cytochrome P450 leading to ripple effects in metabolic disorders such as atypical steroid processing in metabolic syndrome indicating the importance of DHRS7 in maintaining normal cellular physiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including steroids, retinoids and xenobiotics (PubMed : 24246760, PubMed : 26466768, PubMed : 28457967, PubMed : 28687384). Catalyzes the reduction/inactivation of 5alpha-dihydrotestosterone to 3alpha-androstanediol, with a possible role in the modulation of androgen receptor function (PubMed : 28457967, PubMed : 28687384). Involved in the reduction of all-trans-retinal to all-trans-retinol (PubMed : 26466768). Converts cortisone to 20beta-dihydrocortisone in vitro, although the physiological relevance of this activity is questionable (PubMed : 28457967). Reduces exogenous compounds such as quinones (1,2-naphtoquinone, 9,10-phenantrenequinone and benzoquinone) and other xenobiotics (alpha-diketones) in vitro, suggesting a role in the biotransformation of xenobiotics with carbonyl group (PubMed : 24246760, PubMed : 26466768). A dehydrogenase activity has not been detected so far (PubMed : 24246760). May play a role as tumor suppressor (PubMed : 26311046).

See full target information DHRS7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

Western blot - Anti-DHRS7 antibody [EPR9339(B)] - BSA and Azide free (AB249260)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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