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AB220098

Anti-DHTKD1 antibody

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(1 Publication)

Rabbit Polyclonal DHTKD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHTKD1 aa 1-150.

View Alternative Names

KIAA1630, DHTKD1, 2-oxoadipate dehydrogenase complex component E1, E1a, OADC-E1, OADH-E1, Alpha-ketoadipate dehydrogenase, Dehydrogenase E1 and transketolase domain-containing protein 1, Alpha-KADH-E1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB220098)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB220098)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DHTKD1 with ab220098 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DHTKD1 antibody (AB220098)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHTKD1 antibody (AB220098)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling DHTKD1 with ab220098 at 4 μg/ml (green).

Western blot - Anti-DHTKD1 antibody (AB220098)
  • WB

Supplier Data

Western blot - Anti-DHTKD1 antibody (AB220098)

All lanes:

Western blot - Anti-DHTKD1 antibody (ab220098) at 1/100 dilution

All lanes:

RT-4 cell extract

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DHTKD1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q96HY7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHTKD1 also known as dehydrogenase E1 and transketolase domain containing 1 is a mitochondrial protein with a mass of approximately 100 kDa. Its role involves the catalysis of oxidative decarboxylation of 2-oxoadipate in the lysine and tryptophan degradation pathways making it important for mitochondrial energy metabolism. DHTKD1 expression occurs broadly in human tissues with higher levels in organs with high metabolic demands like muscle and liver.
Biological function summary

DHTKD1 operates as part of the 2-oxoadipate dehydrogenase complex which is multi-enzymatic. This complex works in tandem to convert 2-oxoadipate into glutaryl-CoA an essential process in the catabolic pathways of lysine hydroxylysine and tryptophan. By working alongside other enzymes like dihydrolipoamide dehydrogenase DHTKD1 supports the intricate network of energy production and biosynthesis in cells.

Pathways

DHTKD1 plays a pivotal role in the lysine and tryptophan catabolic pathways contributing significantly to the production of ketone bodies and energy. It is closely related to proteins such as OGDH (oxoglutarate dehydrogenase) and BCKAD (branched-chain alpha-keto acid dehydrogenase complex) which participate in various metabolic cycles essential for cellular respiration and regulation of amino acid levels.

DHTKD1 mutations have been linked to 2-aminoadipic and 2-oxoadipic aciduria a metabolic disorder characterized by the accumulation of 2-oxoadipate and L-2-hydroxyglutarate leading to developmental delay and motor dysfunction. It is also associated with Charcot-Marie-Tooth disease (CMT) where it may affect peripheral nerve function. Both conditions underline the protein's importance in maintaining metabolic and neurological health highlighting its interaction with other key proteins involved in mitochondrial dynamics and nervous system integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

2-oxoadipate dehydrogenase (E1a) component of the 2-oxoadipate dehydrogenase complex (OADHC) (PubMed : 29191460, PubMed : 29752936, PubMed : 32303640, PubMed : 32633484, PubMed : 32695416). Participates in the first step, rate limiting for the overall conversion of 2-oxoadipate (alpha-ketoadipate) to glutaryl-CoA and CO(2) catalyzed by the whole OADHC (PubMed : 29191460, PubMed : 32695416). Catalyzes the irreversible decarboxylation of 2-oxoadipate via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST) (Probable) (PubMed : 29752936, PubMed : 32303640, PubMed : 32633484). Can catalyze the decarboxylation of 2-oxoglutarate in vitro, but at a much lower rate than 2-oxoadipate (PubMed : 29191460, PubMed : 29752936, PubMed : 32633484, PubMed : 32695416). Responsible for the last step of L-lysine, L-hydroxylysine and L-tryptophan catabolism with the common product being 2-oxoadipate (Probable).
See full target information DHTKD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 17:23545-23567 PubMed37988765

2023

Single-Component Multilayered Self-Assembling Protein Nanoparticles Displaying Extracellular Domains of Matrix Protein 2 as a Pan-influenza A Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Keegan Braz Gomes,Yi-Nan Zhang,Yi-Zong Lee,Mor Eldad,Alexander Lim,Garrett Ward,Sarah Auclair,Linling He,Jiang Zhu
View all publications
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Product promise

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