AB230392

Anti-DHTKD1 antibody

Name: Anti-DHTKD1 antibody (ab230392) | Abcam
Brand: Abcam Limited
SKU: AB230392
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DHTKD1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHTKD1 aa 1-300.

View Alternative Names

KIAA1630, DHTKD1, 2-oxoadipate dehydrogenase complex component E1, E1a, OADC-E1, OADH-E1, Alpha-ketoadipate dehydrogenase, Dehydrogenase E1 and transketolase domain-containing protein 1, Alpha-KADH-E1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

Paraffin-embedded human gastric cancer tissue stained for DHTKD1 using ab230392 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

Paraffin-embedded human brain tissue stained for DHTKD1 using ab230392 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DHTKD1 antibody (AB230392)

All lanes:

Western blot - Anti-DHTKD1 antibody (ab230392) at 1.82 µg/mL

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 103 kDa

Observed band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DHTKD1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q96HY7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHTKD1 also known as dehydrogenase E1 and transketolase domain containing 1 is a mitochondrial protein with a mass of approximately 100 kDa. Its role involves the catalysis of oxidative decarboxylation of 2-oxoadipate in the lysine and tryptophan degradation pathways making it important for mitochondrial energy metabolism. DHTKD1 expression occurs broadly in human tissues with higher levels in organs with high metabolic demands like muscle and liver.

Biological function summary

DHTKD1 operates as part of the 2-oxoadipate dehydrogenase complex which is multi-enzymatic. This complex works in tandem to convert 2-oxoadipate into glutaryl-CoA an essential process in the catabolic pathways of lysine hydroxylysine and tryptophan. By working alongside other enzymes like dihydrolipoamide dehydrogenase DHTKD1 supports the intricate network of energy production and biosynthesis in cells.

Pathways

DHTKD1 plays a pivotal role in the lysine and tryptophan catabolic pathways contributing significantly to the production of ketone bodies and energy. It is closely related to proteins such as OGDH (oxoglutarate dehydrogenase) and BCKAD (branched-chain alpha-keto acid dehydrogenase complex) which participate in various metabolic cycles essential for cellular respiration and regulation of amino acid levels.

DHTKD1 mutations have been linked to 2-aminoadipic and 2-oxoadipic aciduria a metabolic disorder characterized by the accumulation of 2-oxoadipate and L-2-hydroxyglutarate leading to developmental delay and motor dysfunction. It is also associated with Charcot-Marie-Tooth disease (CMT) where it may affect peripheral nerve function. Both conditions underline the protein's importance in maintaining metabolic and neurological health highlighting its interaction with other key proteins involved in mitochondrial dynamics and nervous system integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

2-oxoadipate dehydrogenase (E1a) component of the 2-oxoadipate dehydrogenase complex (OADHC) (PubMed : 29191460, PubMed : 29752936, PubMed : 32303640, PubMed : 32633484, PubMed : 32695416). Participates in the first step, rate limiting for the overall conversion of 2-oxoadipate (alpha-ketoadipate) to glutaryl-CoA and CO(2) catalyzed by the whole OADHC (PubMed : 29191460, PubMed : 32695416). Catalyzes the irreversible decarboxylation of 2-oxoadipate via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST) (Probable) (PubMed : 29752936, PubMed : 32303640, PubMed : 32633484). Can catalyze the decarboxylation of 2-oxoglutarate in vitro, but at a much lower rate than 2-oxoadipate (PubMed : 29191460, PubMed : 29752936, PubMed : 32633484, PubMed : 32695416). Responsible for the last step of L-lysine, L-hydroxylysine and L-tryptophan catabolism with the common product being 2-oxoadipate (Probable).

See full target information DHTKD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed32897880

2020

Role of defective calcium regulation in cardiorespiratory dysfunction in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Haikel Dridi,Xiaoping Liu,Qi Yuan,Steve Reiken,Mohamad Yehia,Leah Sittenfeld,Panagiota Apostolou,Julie Buron,Pierre Sicard,Stefan Matecki,Jérome Thireau,Clement Menuet,Alain Lacampagne,Andrew R Marks

Molecular cell 76:660-675.e9 PubMed31542297

2019

Glutarylation of Histone H4 Lysine 91 Regulates Chromatin Dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Xiucong Bao,Zheng Liu,Wei Zhang,Kornelia Gladysz,Yi Man Eva Fung,Gaofei Tian,Ying Xiong,Jason Wing Hon Wong,Karen Wing Yee Yuen,Xiang David Li

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHTKD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

Western blot - Anti-DHTKD1 antibody (AB230392)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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