AB254591

Anti-DHX15/prp43 antibody

Name: Anti-DHX15/prp43 antibody (ab254591) | Abcam
Brand: Abcam Limited
SKU: AB254591
Rating: 5 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal DHX15/prp43 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHX15 aa 50-150.

View Alternative Names

DBP1, DDX15, DHX15, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46, DEAH box protein 15, Splicing factor Prp43, hPrp43

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-DHX15/prp43 antibody (AB254591)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DHX15/prp43 antibody (AB254591)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling DHX15/prp43 using ab254591 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Paraffin-embedded human rectum tissue stained for DHX15/prp43 using ab254591 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Paraffin-embedded human testis tissue stained for DHX15/prp43 using ab254591 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Paraffin-embedded human fallopian tube tissue stained for DHX15/prp43 using ab254591 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Paraffin-embedded human cerebellum tissue stained for DHX15/prp43 using ab254591 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DHX15/prp43 antibody (AB254591)

All lanes:

Western blot - Anti-DHX15/prp43 antibody (ab254591) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line0 cell lysate

Lane 2:

NBT II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 91 kDa

false

Supplier Data

Western blot - Anti-DHX15/prp43 antibody (AB254591)

All lanes:

Western blot - Anti-DHX15/prp43 antibody (ab254591) at 0.04 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DHX15 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

O43143

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX15 also known as DEAH-Box Protein 15 or prp43 forms part of the DExH/D-box helicase family. This protein functions in the ATP-dependent unwinding of RNA and has a molecular mass of approximately 95 kDa. Expression of DHX15 is found in various tissues with high levels in heart and skeletal muscle. The protein is involved in the splicing of pre-mRNA and rRNA processing where its enzymatic activities ensure the proper modification and maturation of RNA molecules.

Biological function summary

The splicing complex involves DHX15 as an important component. This protein plays a role in the disassembly of spliceosomes leading to spliceosome recycling essential for efficient mRNA processing. DHX15 associates with other proteins within the spliceosome helping to coordinate the removal of introns from pre-mRNA. As part of the broader RNA processing machinery DHX15 ensures the accurate and efficient production of mature RNA necessary for proper gene expression and cellular function.

Pathways

DHX15 participates in the mRNA splicing and ribosome biogenesis pathways. It works alongside related proteins like SF3B1 within the spliceosome for mRNA splicing. Ribosome biogenesis involves DHX15 acting in concert with several factors to facilitate rRNA maturation impacting protein synthesis capacities of cells. These pathways which require precise RNA processing and protein assembly highlight DHX15's roles in cellular metabolism and regulatory mechanisms.

DHX15 associates with myelodysplastic syndrome and congenital heart defects. Mutations or dysregulation of DHX15 disrupt RNA processing contributing to these conditions. The protein SF3B1 interacts with DHX15 in myelodysplastic syndrome. In congenital heart defects disrupted DHX15 function correlates with improper heart and vascular formation during development. Understanding these associations points to the significance of DHX15 in maintaining RNA processing fidelity and its broader implications in human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA helicase involved in mRNA processing and antiviral innate immunity (PubMed : 19103666, PubMed : 19432882, PubMed : 24782566, PubMed : 24990078, PubMed : 32179686, PubMed : 34161762). Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA (PubMed : 19103666). In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (PubMed : 19103666). Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome (PubMed : 24782566, PubMed : 24990078, PubMed : 34161762). Acts as an RNA virus sensor : recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3) (PubMed : 24782566, PubMed : 24990078, PubMed : 34161762). Involved in intestinal antiviral innate immunity together with NLRP6 : recognizes and binds viral dsRNA and promotes activation of the NLRP6 inflammasome in intestinal epithelial cells to restrict infection by enteric viruses (PubMed : 34161762). The NLRP6 inflammasome acts by promoting maturation and secretion of IL18 in the extracellular milieu (PubMed : 34161762). Also involved in antibacterial innate immunity by promoting Wnt-induced antimicrobial protein expression in Paneth cells (By similarity).

See full target information DHX15

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114405 PubMed38923463

2024

CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Radoslaw Lukoszek,Francisco Inesta-Vaquera,Natasha J M Brett,Shang Liang,Lydia A Hepburn,David J Hughes,Chiara Pirillo,Edward W Roberts,Victoria H Cowling

Cell reports 42:113223 PubMed37805921

2023

Splicing quality control mediated by DHX15 and its G-patch activator SUGP1.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Feng,Keegan Krick,Jennifer Chu,Christopher B Burge

Molecular cell 83:2258-2275.e11 PubMed37369199

2023

A functional link between lariat debranching enzyme and the intron-binding complex is defective in non-photosensitive trichothiodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany A Townley,Luke Buerer,Ning Tsao,Albino Bacolla,Fadhel Mansoori,Timur Rusanov,Nathanial Clark,Negar Goodarzi,Nicolas Schmidt,Sridhar Nonavinkere Srivatsan,Hua Sun,Reilly A Sample,Joshua R Brickner,Drew McDonald,Miaw-Sheue Tsai,Matthew J Walter,David F Wozniak,Alex S Holehouse,Vladimir Pena,John A Tainer,William G Fairbrother,Nima Mosammaparast

Nucleic acids research 50:2905-2922 PubMed35212377

2022

CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Liang,Joana C Silva,Olga Suska,Radoslaw Lukoszek,Rajaei Almohammed,Victoria H Cowling

Cancers 13: PubMed34771722

2021

VISTA Is a Diagnostic Biomarker and Immunotherapy Target of Aggressive Feline Mammary Carcinoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Gameiro,Catarina Nascimento,Jorge Correia,Fernando Ferreira

Cell reports 35:109205 PubMed34161762

2021

DHX15 is required to control RNA virus-induced intestinal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Junji Xing,Xiaojing Zhou,Mingli Fang,Evan Zhang,Laurie J Minze,Zhiqiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX15/prp43 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DHX15/prp43 antibody (AB254591)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX15/prp43 antibody (AB254591)

Western blot - Anti-DHX15/prp43 antibody (AB254591)

Western blot - Anti-DHX15/prp43 antibody (AB254591)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise