Rabbit Polyclonal DHX15/prp43 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DHX15.
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Publications
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- Haematologica 108:2029-20432023RNA helicase DHX15 exemplifies a unique dependency in acute leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Guo,Jin Xu,Peiqi Xing,Qilong Li,Donghai Wang,Chao Tang,Bruno Palhais,Juliette Roels,Jiaxu Liu,Sa Pan,Jinyan Huang,Zhaoqi Liu,Ping Zhu,Tom Taghon,Guoliang Qing,Pieter Van Vlierberghe,Hudan LiuPubMed 36861414
- Cellular & molecular immunology 19:687-7012022The RNA helicase DHX15 is a critical regulator of natural killer-cell homeostasis and functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangchuan Wang,Xiang Xiao,Yixuan Wang,Xiufeng Chu,Yaling Dou,Laurie J Minze,Rafik M Ghobrial,Zhiqiang Zhang,Xian C LiPubMed 35322175
- RNA (New York, N.Y.) 28:583-5952022A model for DHX15 mediated disassembly of A-complex spliceosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah M Maul-Newby,Angela N Amorello,Turvi Sharma,John H Kim,Matthew S Modena,Beth E Prichard,Melissa S JuricaPubMed 35046126
- Frontiers in medicine 7:5917362021DHX15 Inhibits Autophagy and the Proliferation of Hepatoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiaomiao Zhao,Lixiong Ying,Rusha Wang,Jiping Yao,Liming Zhu,Min Zheng,Zhi Chen,Zhenggang YangPubMed 33644083
- Journal of interferon & cytokine research : the of 39:331-3462019DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSowmya Pattabhi et. al.PubMed 31090472
- Life science alliance 1:e2018000922018DHX15 regulates CMTR1-dependent gene expression and cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Inesta-Vaquera,Viduth K Chaugule,Alison Galloway,Laurel Chandler,Alejandro Rojas-Fernandez,Simone Weidlich,Mark Peggie,Victoria H CowlingPubMed 30079402
- Nature 546:667-6702017Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Zhu et. al.PubMed 28636595
- Nucleic acids research 42:7330-452014The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DHX15. The exact immunogen used to generate this antibody is proprietary information. Database link O43143
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Platypus | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Turkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Pig, Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/15000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Pig, Rabbit | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
RNA helicase involved in mRNA processing and antiviral innate immunity (PubMed:19103666, PubMed:19432882, PubMed:24782566, PubMed:24990078, PubMed:32179686, PubMed:34161762). Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA (PubMed:19103666). In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (PubMed:19103666). Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome (PubMed:24782566, PubMed:24990078, PubMed:34161762). Acts as an RNA virus sensor: recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3) (PubMed:24782566, PubMed:24990078, PubMed:34161762). Involved in intestinal antiviral innate immunity together with NLRP6: recognizes and binds viral dsRNA and promotes activation of the NLRP6 inflammasome in intestinal epithelial cells to restrict infection by enteric viruses (PubMed:34161762). The NLRP6 inflammasome acts by promoting maturation and secretion of IL18 in the extracellular milieu (PubMed:34161762). Also involved in antibacterial innate immunity by promoting Wnt-induced antimicrobial protein expression in Paneth cells (By similarity).
Alternative names
DBP1, DDX15, DHX15, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46, DEAH box protein 15, Splicing factor Prp43, hPrp43
Recommended products
Rabbit Polyclonal DHX15/prp43 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human DHX15.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DHX15. The exact immunogen used to generate this antibody is proprietary information. Database link O43143
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab70454 was affinity purified using an epitope specific to DHX15/prp43 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DHX15 also known as DEAH-Box Protein 15 or prp43 forms part of the DExH/D-box helicase family. This protein functions in the ATP-dependent unwinding of RNA and has a molecular mass of approximately 95 kDa. Expression of DHX15 is found in various tissues with high levels in heart and skeletal muscle. The protein is involved in the splicing of pre-mRNA and rRNA processing where its enzymatic activities ensure the proper modification and maturation of RNA molecules.
- Biological function summary
The splicing complex involves DHX15 as an important component. This protein plays a role in the disassembly of spliceosomes leading to spliceosome recycling essential for efficient mRNA processing. DHX15 associates with other proteins within the spliceosome helping to coordinate the removal of introns from pre-mRNA. As part of the broader RNA processing machinery DHX15 ensures the accurate and efficient production of mature RNA necessary for proper gene expression and cellular function.
- Pathways
DHX15 participates in the mRNA splicing and ribosome biogenesis pathways. It works alongside related proteins like SF3B1 within the spliceosome for mRNA splicing. Ribosome biogenesis involves DHX15 acting in concert with several factors to facilitate rRNA maturation impacting protein synthesis capacities of cells. These pathways which require precise RNA processing and protein assembly highlight DHX15's roles in cellular metabolism and regulatory mechanisms.
- Associated diseases and disorders
DHX15 associates with myelodysplastic syndrome and congenital heart defects. Mutations or dysregulation of DHX15 disrupt RNA processing contributing to these conditions. The protein SF3B1 interacts with DHX15 in myelodysplastic syndrome. In congenital heart defects disrupted DHX15 function correlates with improper heart and vascular formation during development. Understanding these associations points to the significance of DHX15 in maintaining RNA processing fidelity and its broader implications in human health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-DHX15/prp43 antibody (ab70454)
All lanes: Western blot - Anti-DHX15/prp43 antibody (ab70454) at 0.1 µg/mL
All lanes: Whole cell lysate from 293T cells. at 60 µg
Predicted band size: 91 kDa
Observed band size: 97 kDa
Immunoprecipitation - Anti-DHX15/prp43 antibody (ab70454)
1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.
All lanes: Immunoprecipitation - Anti-DHX15/prp43 antibody (ab70454)
Predicted band size: 91 kDa
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