AB70454

Anti-DHX15/prp43 antibody

Name: Anti-DHX15/prp43 antibody (ab70454) | Abcam
Brand: Abcam Limited
SKU: AB70454

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(10 Publications)

Rabbit Polyclonal DHX15/prp43 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DHX15.

View Alternative Names

DBP1, DDX15, DHX15, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46, DEAH box protein 15, Splicing factor Prp43, hPrp43

2 Images

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Immunoprecipitation - Anti-DHX15/prp43 antibody (AB70454)

1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.

All lanes:

Immunoprecipitation - Anti-DHX15/prp43 antibody (ab70454)

Predicted band size: 91 kDa

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Western blot - Anti-DHX15/prp43 antibody (AB70454)

All lanes:

Western blot - Anti-DHX15/prp43 antibody (ab70454) at 0.1 µg/mL

All lanes:

Whole cell lysate from 293T cells. at 60 µg

Predicted band size: 91 kDa

Observed band size: 97 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DHX15. The exact immunogen used to generate this antibody is proprietary information.

O43143

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70454 was affinity purified using an epitope specific to DHX15/prp43 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX15 also known as DEAH-Box Protein 15 or prp43 forms part of the DExH/D-box helicase family. This protein functions in the ATP-dependent unwinding of RNA and has a molecular mass of approximately 95 kDa. Expression of DHX15 is found in various tissues with high levels in heart and skeletal muscle. The protein is involved in the splicing of pre-mRNA and rRNA processing where its enzymatic activities ensure the proper modification and maturation of RNA molecules.

Biological function summary

The splicing complex involves DHX15 as an important component. This protein plays a role in the disassembly of spliceosomes leading to spliceosome recycling essential for efficient mRNA processing. DHX15 associates with other proteins within the spliceosome helping to coordinate the removal of introns from pre-mRNA. As part of the broader RNA processing machinery DHX15 ensures the accurate and efficient production of mature RNA necessary for proper gene expression and cellular function.

Pathways

DHX15 participates in the mRNA splicing and ribosome biogenesis pathways. It works alongside related proteins like SF3B1 within the spliceosome for mRNA splicing. Ribosome biogenesis involves DHX15 acting in concert with several factors to facilitate rRNA maturation impacting protein synthesis capacities of cells. These pathways which require precise RNA processing and protein assembly highlight DHX15's roles in cellular metabolism and regulatory mechanisms.

DHX15 associates with myelodysplastic syndrome and congenital heart defects. Mutations or dysregulation of DHX15 disrupt RNA processing contributing to these conditions. The protein SF3B1 interacts with DHX15 in myelodysplastic syndrome. In congenital heart defects disrupted DHX15 function correlates with improper heart and vascular formation during development. Understanding these associations points to the significance of DHX15 in maintaining RNA processing fidelity and its broader implications in human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA helicase involved in mRNA processing and antiviral innate immunity (PubMed : 19103666, PubMed : 19432882, PubMed : 24782566, PubMed : 24990078, PubMed : 32179686, PubMed : 34161762). Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA (PubMed : 19103666). In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (PubMed : 19103666). Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome (PubMed : 24782566, PubMed : 24990078, PubMed : 34161762). Acts as an RNA virus sensor : recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3) (PubMed : 24782566, PubMed : 24990078, PubMed : 34161762). Involved in intestinal antiviral innate immunity together with NLRP6 : recognizes and binds viral dsRNA and promotes activation of the NLRP6 inflammasome in intestinal epithelial cells to restrict infection by enteric viruses (PubMed : 34161762). The NLRP6 inflammasome acts by promoting maturation and secretion of IL18 in the extracellular milieu (PubMed : 34161762). Also involved in antibacterial innate immunity by promoting Wnt-induced antimicrobial protein expression in Paneth cells (By similarity).

See full target information DHX15

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108571 PubMed38161423

2024

A helicase-independent role of DHX15 promotes MYC stability and acute leukemia cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Qilong Li,Hao Guo,Jin Xu,Xinlu Li,Donghai Wang,Ying Guo,Guoliang Qing,Pieter Van Vlierberghe,Hudan Liu

Haematologica 108:2029-2043 PubMed36861414

2023

RNA helicase DHX15 exemplifies a unique dependency in acute leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Guo,Jin Xu,Peiqi Xing,Qilong Li,Donghai Wang,Chao Tang,Bruno Palhais,Juliette Roels,Jiaxu Liu,Sa Pan,Jinyan Huang,Zhaoqi Liu,Ping Zhu,Tom Taghon,Guoliang Qing,Pieter Van Vlierberghe,Hudan Liu

Cellular & molecular immunology 19:687-701 PubMed35322175

2022

The RNA helicase DHX15 is a critical regulator of natural killer-cell homeostasis and functions.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchuan Wang,Xiang Xiao,Yixuan Wang,Xiufeng Chu,Yaling Dou,Laurie J Minze,Rafik M Ghobrial,Zhiqiang Zhang,Xian C Li

RNA (New York, N.Y.) 28:583-595 PubMed35046126

2022

A model for DHX15 mediated disassembly of A-complex spliceosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Maul-Newby,Angela N Amorello,Turvi Sharma,John H Kim,Matthew S Modena,Beth E Prichard,Melissa S Jurica

Frontiers in medicine 7:591736 PubMed33644083

2021

DHX15 Inhibits Autophagy and the Proliferation of Hepatoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Zhao,Lixiong Ying,Rusha Wang,Jiping Yao,Liming Zhu,Min Zheng,Zhi Chen,Zhenggang Yang

Biochimica et biophysica acta. Gene regulatory mec 1863:194624 PubMed32798738

2020

The lysine demethylase KDM4A controls the cell-cycle expression of replicative canonical histone genes.

Applications

Unspecified application

Species

Unspecified reactive species

Capucine Van Rechem,Fei Ji,Sweta Mishra,Damayanti Chakraborty,Sedona E Murphy,Megan E Dillingham,Ruslan I Sadreyev,Johnathan R Whetstine

Journal of interferon & cytokine research : the of 39:331-346 PubMed31090472

2019

DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Sowmya Pattabhi,Megan L Knoll,Michael Gale,Yueh-Ming Loo

Life science alliance 1:e201800092 PubMed30079402

2018

DHX15 regulates CMTR1-dependent gene expression and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Inesta-Vaquera,Viduth K Chaugule,Alison Galloway,Laurel Chandler,Alejandro Rojas-Fernandez,Simone Weidlich,Mark Peggie,Victoria H Cowling

Nature 546:667-670 PubMed28636595

2017

Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Zhu,Siyuan Ding,Penghua Wang,Zheng Wei,Wen Pan,Noah W Palm,Yi Yang,Hua Yu,Hua-Bing Li,Geng Wang,Xuqiu Lei,Marcel R de Zoete,Jun Zhao,Yunjiang Zheng,Haiwei Chen,Yujiao Zhao,Kellie A Jurado,Ningguo Feng,Liang Shan,Yuval Kluger,Jun Lu,Clara Abraham,Erol Fikrig,Harry B Greenberg,Richard A Flavell

Nucleic acids research 42:7330-45 PubMed24823796

2014

The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis.

Applications

Species

Human

Yan-Ling Chen,Régine Capeyrou,Odile Humbert,Saïda Mouffok,Yasmine Al Kadri,Simon Lebaron,Anthony K Henras,Yves Henry

View all publications

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Product promise

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Anti-DHX15/prp43 antibody

Immunoprecipitation - Anti-DHX15/prp43 antibody (AB70454)

Western blot - Anti-DHX15/prp43 antibody (AB70454)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise