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AB176819

Anti-DHX16 antibody

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(1 Publication)

Rabbit Polyclonal DHX16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DHX16 aa 950 to C-terminus.

View Alternative Names

DBP2, DDX16, KIAA0577, PRP2, DHX16, Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, ATP-dependent RNA helicase #3, DEAH-box protein 16

2 Images
Immunoprecipitation - Anti-DHX16 antibody (AB176819)
  • IP

Supplier Data

Immunoprecipitation - Anti-DHX16 antibody (AB176819)

Detection of DHX16 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176819 at 3 μg/mg lysate for IP (Lane 1). For WB detection ab176819 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-DHX16 antibody (ab176819)

Predicted band size: 119 kDa

false

Western blot - Anti-DHX16 antibody (AB176819)
  • WB

Supplier Data

Western blot - Anti-DHX16 antibody (AB176819)

All lanes:

Western blot - Anti-DHX16 antibody (ab176819) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 119 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DHX16 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60231

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein DHX16 also known as DBP2 is an ATP-dependent RNA helicase belonging to the DEAH-box family. It has a molecular mass of around 130 kDa. RNA helicases like DHX16 unwind RNA structures aiding in RNA processing events. You can find DHX16 in the nucleus where it participates in the modification and maturation of pre-mRNAs.
Biological function summary

DHX16 plays a role in RNA splicing by participating in the spliceosome complex. This complex carries out the splicing of pre-mRNA introns an important step for producing mature mRNAs. By unwinding RNA structures DHX16 helps in the precise removal and rearrangement of introns and exons. The efficient function of DHX16 in the spliceosome ensures accurate genetic expression affecting various cellular functions.

Pathways

DHX16 influences the mRNA processing and splicing pathway possessing a significant relation to protein synthesis. The spliceosome where DHX16 functions acts in tandem with various other splicing factors such as SF3B1 and U2AF65 ensuring the controlled and regulated maturation of mRNA transcripts. This activity links DHX16 to gene expression pathways impacting cell development and cycle regulation.

DHX16 associates with certain genetic abnormalities and cancers. Mutations or misregulation of DHX16 can contribute to impaired RNA processing tying it to disorders such as myelodysplastic syndromes (MDS). In MDS abnormal splicing mechanisms induced by DHX16 anomalies can lead to ineffective hematopoiesis. Furthermore DHX16 might interact with proteins like SRSF2 in these conditions revealing a broader network of splicing factors involved in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for pre-mRNA splicing as a component of the spliceosome (PubMed : 20423332, PubMed : 20841358, PubMed : 25296192, PubMed : 29360106). Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA (PubMed : 35263596). Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses (PubMed : 35263596).
See full target information DHX16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 40:88 PubMed33648545

2021

Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Su,Shikai Zhu,Kai Chen,Hongji Yang,Mingwu Tian,Qiang Fu,Ganggang Shi,Shijian Feng,Dianyun Ren,Xin Jin,Chong Yang
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Product promise

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