AB85687

Anti-DHX30 antibody

Name: Anti-DHX30 antibody (ab85687) | Abcam
Brand: Abcam Limited
SKU: AB85687

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(10 Publications)

Rabbit Polyclonal DHX30 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DHX30 aa 350-400.

View Alternative Names

DDX30, KIAA0890, DHX30, ATP-dependent RNA helicase DHX30, DEAH box protein 30

3 Images

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Immunoprecipitation - Anti-DHX30 antibody (AB85687)

Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.

All lanes:

Immunoprecipitation - Anti-DHX30 antibody (ab85687)

Predicted band size: 134 kDa

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Unknown

Western blot - Anti-DHX30 antibody (AB85687)

All lanes:

Western blot - Anti-DHX30 antibody (ab85687) at 0.04 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 134 kDa

Observed band size: 110 kDa,134 kDa,238 kDa,60 kDa

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CiteAb

Western blot - Anti-DHX30 antibody (AB85687)

DHX30 western blot using anti-DHX30 antibody ab85687. Publication image and figure legend from Murat, P., Marsico, G., et al., 2018, Genome Biol, PubMed 30591072.

ab85687 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab85687 please see the product overview.

Polysome and ribosome profiling defines the role of DHX36 and DHX9 on translation. a Polysome profiling of HeLa cytoplasmic extract coupled with mass spectrometry allowed estimating the enrichment of different helicases in monosome or polysome fractions. Duplicates were used to quantitatively estimate the presence of a given helicase in each fraction. A hierarchical cluster analysis reporting the distribution of human helicases within mono/polysomes is shown. b Immunoblots of a polysome profile probing for members of the DEAH-box/RHA helicase family (left blot) confirmed the presence of DHX36 and DHX9 in both light and heavy polysomes. On the right is reported the result of affinity purifications using indicated biotinylated nucleic acids probes showing the presence of DHX9, DHX36, and DHX57 in rG4/ribonucleoprotein complexes. Lysate : 30 μg of total protein, Neg : empty beads, mut G4 : mutated rG4-forming sequences, rG4 : rG4-forming sequences, SL : stem-loop forming sequence. c Immunoblots of siRNA-treated cells probing DHX36 and DHX9 show that siRNA depletion of both helicases is reproducible and selective. Rep : replicate. Ribosome profiling allowed assessing change in TE upon depletion of d DHX36 and e DHX9. Are reported the frequency distributions of the ratio of TE in DHX36 (top) and DHX9 (bottom) depleted cells over TE in control (n = 3 replicates). f Comparison of fold changes of TE upon DHX36 and DHX9 depletion. Q values were calculated by combining P values using Fisher's method. g Anticorrelation between fold changes of TE and fold changes of RPFdist upon depletion of DHX36 (red) or DHX9 (blue) for transcripts showing significant change in TE (Q value < 0.05)

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DHX30 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q7L2E3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX30 also known as DExH-Box Helicase 30 is a member of the DEAD-box helicase family and plays a role in RNA metabolism. This protein has a mass of approximately 132 kDa and is widely expressed across various tissues with notable abundance in the brain and testes. Its core mechanical function includes unwinding RNA structures which is critical for the assembly and remodeling of ribonucleoprotein complexes. By doing so DHX30 facilitates various steps in RNA processing including transcription splicing and translation initiation.

Biological function summary

Within cellular processes DHX30 contributes to mitochondrial function and protein synthesis by participating in RNA splicing and translation. It forms part of larger ribonucleoprotein complexes that regulate RNA dynamics. The protein interacts with other cellular components to coordinate the expression of genes involved in cellular growth and metabolism. Its presence in the mitochondrial matrix indicates a specific role in maintaining mitochondrial health and efficient energy production.

Pathways

The DHX30 protein influences cellular activities related to RNA-binding and translation regulation. It is deeply integrated within the translation initiation pathway working alongside RNA-binding proteins to regulate gene expression efficiently. Within this context DHX30 associates with proteins such as EIF4A which is involved in initiating translation of mRNA. Moreover DHX30 participates in maintaining proper functioning of the mitochondrial translation machinery ensuring the synthesis of essential mitochondrial proteins that power the cell's energy requirements.

DHX30 has links to intellectual disabilities and neurodegenerative disorders. Variants or deficiencies in DHX30's function can contribute to conditions such as neurodevelopmental disorders which could potentially connect with other proteins like FMRP involved in synaptic function and mental development. Additionally disruptions in DHX30 activity have been observed in certain types of cancers where aberrant RNA processing and gene expression stability are important factors. Understanding these associations may provide insights into the role of DHX30 in maintaining cellular integrity and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

RNA-dependent helicase (PubMed : 29100085). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (PubMed : 25683715, PubMed : 29100085). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (PubMed : 18063578). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).

See full target information DHX30

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2411298122 PubMed39752519

2025

Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA production.

Applications

Unspecified application

Species

Unspecified reactive species

Xihui Xu,Rozhin Penjweini,Lóránt Székvölgyi,Zsolt Karányi,Anne-Marie Heckel,Devikala Gurusamy,Dóra Varga,Shutong Yang,Alexandra L Brown,Wenqi Cui,Jinsung Park,Dénes Nagy,Maren C Podszun,Sarah Yang,Komudi Singh,Stephen P Ashcroft,Jeonghan Kim,Myung K Kim,Ivan Tarassov,Jun Zhu,Andrew Philp,Yaron Rotman,Jay R Knutson,Nina Entelis,Jay H Chung

The journal of headache and pain 25:28 PubMed38433184

2024

LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion.

Applications

Unspecified application

Species

Unspecified reactive species

YaJing Liu,Fei Liu,YiKe Li,YueLing Li,YuHeng Feng,JiaShuo Zhao,Cheng Zhou,ChunJie Li,JieFei Shen,YanYan Zhang

Journal of virology 96:e0112122 PubMed36000840

2022

Seneca Valley Virus Induces DHX30 Cleavage to Antagonize Its Antiviral Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wen,Zixuan Zheng,Haoyuan Wang,Qiongqiong Zhao,Mengge Yin,Huanchun Chen,Xiangmin Li,Ping Qian

PLoS genetics 17:e1009873 PubMed34748562

2021

The FASTK family proteins fine-tune mitochondrial RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Ohkubo,Lindsey Van Haute,Danielle L Rudler,Maike Stentenbach,Florian A Steiner,Oliver Rackham,Michal Minczuk,Aleksandra Filipovska,Jean-Claude Martinou

Cells 9: PubMed32708779

2020

Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha Vassileff,Laura J Vella,Harinda Rajapaksha,Mitch Shambrook,Amirmohammad Nasiri Kenari,Catriona McLean,Andrew F Hill,Lesley Cheng

Cell reports 30:4355-4369.e6 PubMed32234473

2020

Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Rizzotto,Sara Zaccara,Annalisa Rossi,Matthew D Galbraith,Zdenek Andrysik,Ahwan Pandey,Kelly D Sullivan,Alessandro Quattrone,Joaquín M Espinosa,Erik Dassi,Alberto Inga

Nucleic acids research 48:304-315 PubMed31754723

2019

A double-stranded RNA platform is required for the interaction between a host restriction factor and the NS1 protein of influenza A virus.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Chen,Li-Chung Ma,Shanshan Wang,Ryan L Woltz,Emily M Grasso,Gaetano T Montelione,Robert M Krug

Genome biology 19:229 PubMed30591072

2018

RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Murat,Giovanni Marsico,Barbara Herdy,Avazeh T Ghanbarian,Guillem Portella,Shankar Balasubramanian

Nucleic acids research 46:11592-11604 PubMed30256975

2018

Analysis of NRAS RNA G-quadruplex binding proteins reveals DDX3X as a novel interactor of cellular G-quadruplex containing transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Herdy,Clemens Mayer,Dhaval Varshney,Giovanni Marsico,Pierre Murat,Chris Taylor,Clive D'Santos,David Tannahill,Shankar Balasubramanian

EMBO reports 18:28-38 PubMed27974379

2016

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Antonicka,Karine Choquet,Zhen-Yuan Lin,Anne-Claude Gingras,Claudia L Kleinman,Eric A Shoubridge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHX30 antibody

Immunoprecipitation - Anti-DHX30 antibody (AB85687)

Western blot - Anti-DHX30 antibody (AB85687)

Western blot - Anti-DHX30 antibody (AB85687)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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