Rabbit Polyclonal DHX30 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DHX30 aa 350-400.
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Publications
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- Journal of virology 96:e01121222022Seneca Valley Virus Induces DHX30 Cleavage to Antagonize Its Antiviral Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wen,Zixuan Zheng,Haoyuan Wang,Qiongqiong Zhao,Mengge Yin,Huanchun Chen,Xiangmin Li,Ping QianPubMed 36000840
- PLoS genetics 17:e10098732021The FASTK family proteins fine-tune mitochondrial RNA processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Ohkubo,Lindsey Van Haute,Danielle L Rudler,Maike Stentenbach,Florian A Steiner,Oliver Rackham,Michal Minczuk,Aleksandra Filipovska,Jean-Claude MartinouPubMed 34748562
- Cells 9:2020Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasha Vassileff et. al.PubMed 32708779
- Nucleic acids research 48:304-3152019A double-stranded RNA platform is required for the interaction between a host restriction factor and the NS1 protein of influenza A virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuifang Chen,Li-Chung Ma,Shanshan Wang,Ryan L Woltz,Emily M Grasso,Gaetano T Montelione,Robert M KrugPubMed 31754723
- Genome biology 19:2292018RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPierre Murat et. al.PubMed 30591072
- Nucleic acids research 46:11592-116042018Analysis of NRAS RNA G-quadruplex binding proteins reveals DDX3X as a novel interactor of cellular G-quadruplex containing transcripts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Herdy,Clemens Mayer,Dhaval Varshney,Giovanni Marsico,Pierre Murat,Chris Taylor,Clive D'Santos,David Tannahill,Shankar BalasubramanianPubMed 30256975
- EMBO reports 18:28-382016A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Antonicka,Karine Choquet,Zhen-Yuan Lin,Anne-Claude Gingras,Claudia L Kleinman,Eric A ShoubridgePubMed 27974379
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DHX30 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L2E3
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
RNA-dependent helicase (PubMed:29100085). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (PubMed:25683715, PubMed:29100085). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (PubMed:18063578). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).
Alternative names
DDX30, KIAA0890, DHX30, ATP-dependent RNA helicase DHX30, DEAH box protein 30
Recommended products
Rabbit Polyclonal DHX30 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DHX30 aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DHX30 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L2E3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DHX30 also known as DExH-Box Helicase 30 is a member of the DEAD-box helicase family and plays a role in RNA metabolism. This protein has a mass of approximately 132 kDa and is widely expressed across various tissues with notable abundance in the brain and testes. Its core mechanical function includes unwinding RNA structures which is critical for the assembly and remodeling of ribonucleoprotein complexes. By doing so DHX30 facilitates various steps in RNA processing including transcription splicing and translation initiation.
- Biological function summary
Within cellular processes DHX30 contributes to mitochondrial function and protein synthesis by participating in RNA splicing and translation. It forms part of larger ribonucleoprotein complexes that regulate RNA dynamics. The protein interacts with other cellular components to coordinate the expression of genes involved in cellular growth and metabolism. Its presence in the mitochondrial matrix indicates a specific role in maintaining mitochondrial health and efficient energy production.
- Pathways
The DHX30 protein influences cellular activities related to RNA-binding and translation regulation. It is deeply integrated within the translation initiation pathway working alongside RNA-binding proteins to regulate gene expression efficiently. Within this context DHX30 associates with proteins such as EIF4A which is involved in initiating translation of mRNA. Moreover DHX30 participates in maintaining proper functioning of the mitochondrial translation machinery ensuring the synthesis of essential mitochondrial proteins that power the cell's energy requirements.
- Associated diseases and disorders
DHX30 has links to intellectual disabilities and neurodegenerative disorders. Variants or deficiencies in DHX30's function can contribute to conditions such as neurodevelopmental disorders which could potentially connect with other proteins like FMRP involved in synaptic function and mental development. Additionally disruptions in DHX30 activity have been observed in certain types of cancers where aberrant RNA processing and gene expression stability are important factors. Understanding these associations may provide insights into the role of DHX30 in maintaining cellular integrity and its potential as a therapeutic target.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-DHX30 antibody (ab85687)
All lanes: Western blot - Anti-DHX30 antibody (ab85687) at 0.04 µg/mL
Lane 1: HeLa cell lysate at 50 µg
Lane 2: HeLa cell lysate at 15 µg
Lane 3: HeLa cell lysate at 5 µg
Lane 4: 293T cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 110 kDa, 134 kDa, 238 kDa, 60 kDa
Immunoprecipitation - Anti-DHX30 antibody (ab85687)
Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.
All lanes: Immunoprecipitation - Anti-DHX30 antibody (ab85687)
Predicted band size: 134 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-DHX30 antibody (ab85687)
DHX30 western blot using anti-DHX30 antibody ab85687. Publication image and figure legend from Murat, P., Marsico, G., et al., 2018, Genome Biol, PubMed 30591072.
ab85687 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab85687 please see the product overview.
Polysome and ribosome profiling defines the role of DHX36 and DHX9 on translation. a Polysome profiling of HeLa cytoplasmic extract coupled with mass spectrometry allowed estimating the enrichment of different helicases in monosome or polysome fractions. Duplicates were used to quantitatively estimate the presence of a given helicase in each fraction. A hierarchical cluster analysis reporting the distribution of human helicases within mono/polysomes is shown. b Immunoblots of a polysome profile probing for members of the DEAH-box/RHA helicase family (left blot) confirmed the presence of DHX36 and DHX9 in both light and heavy polysomes. On the right is reported the result of affinity purifications using indicated biotinylated nucleic acids probes showing the presence of DHX9, DHX36, and DHX57 in rG4/ribonucleoprotein complexes. Lysate: 30 μg of total protein, Neg: empty beads, mut G4: mutated rG4-forming sequences, rG4: rG4-forming sequences, SL: stem-loop forming sequence. c Immunoblots of siRNA-treated cells probing DHX36 and DHX9 show that siRNA depletion of both helicases is reproducible and selective. Rep: replicate. Ribosome profiling allowed assessing change in TE upon depletion of d DHX36 and e DHX9. Are reported the frequency distributions of the ratio of TE in DHX36 (top) and DHX9 (bottom) depleted cells over TE in control (n = 3 replicates). f Comparison of fold changes of TE upon DHX36 and DHX9 depletion. Q values were calculated by combining P values using Fisher’s method. g Anticorrelation between fold changes of TE and fold changes of RPFdist upon depletion of DHX36 (red) or DHX9 (blue) for transcripts showing significant change in TE (Q value < 0.05)
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